Comparison of a multiplexed MassARRAY system with real-time allele-specific PCR technology for genotyping of methicillin-resistant Staphylococcus aureus

Syrmis, Melanie W.; Moser, Ralf; Whiley, David M.; Vaska, Vikram L.; Coombs, Geoffrey W.; Nissen, Michael D.; Sloots, Theo P.; Nimmo, Graeme R.

doi:10.1111/j.1469-0691.2011.03521.x

Cited by 54 publications

(47 citation statements)

References 23 publications

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“…Next-generation genome sequencing will allow a rapid expansion of SNPs for S. Typhimurium typing, as for S. Typhi (17) and Escherichia coli O157:H7 (6) strains. In this study, we used real-time PCR for typing, but the SNPs can also be used for large-scale typing by the adoption of high-throughput platforms, such as the iPlex MassArray technology (45) or the GoldenGate bead array technology (16). Establishing true relationships based on SNPs would provide a foundation for future genomic studies of differences in the prevalences and virulences of different SNP lineages or phage types.…”

Section: Resultsmentioning

confidence: 99%

Genetic Relationships of Phage Types and Single Nucleotide Polymorphism Typing of Salmonella enterica Serovar Typhimurium

et al. 2012

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Salmonella enterica serovar Typhimurium is one of the leading causes of gastroenteritis in humans. Phage typing has been used for the epidemiological surveillance of S. Typhimurium for over 4 decades. However, knowledge of the evolutionary relationships between phage types is very limited. In this study, we used single nucleotide polymorphisms (SNPs) as molecular markers to determine the relationships between common S. Typhimurium phage types. Forty-four SNPs, including 24 identified in a previous study and 20 from 6 available whole-genome sequences, were used to analyze 215 S. Typhimurium isolates belonging to 45 phage types. Altogether, 215 isolates and 6 genome strains were differentiated into 33 SNP profiles and four distinctive phylogenetic clusters. Fourteen phage types, including DT9, one of the most common phage types in Australia, were differentiated into multiple SNP profiles. These SNP profiles were distributed into different phylogenetic clusters, indicating that they have arisen independently multiple times. This finding suggests that phage typing may not be useful for long-term epidemiological studies over long periods (years) and diverse localities (different countries or continents). SNP typing provided a discriminative power similar to that of phage typing. However, 12 SNP profiles contained more than one phage type, and more SNPs would be needed for further differentiation. SNP typing should be considered as a replacement for phage typing for the identification of S. Typhimurium strains. Salmonella enterica serovar Typhimurium is a broad-host-range pathogen that causes gastroenteritis in humans. The phage typing scheme described by Anderson et al. (3) has been used for the routine epidemiological surveillance of S. Typhimurium infections for many years. Phage types are determined by resistance or sensitivity to a set of 34 phages, and so far, more than 300 definitive phage types (DTs) are recognized (38). Phage typing has been found to be particularly useful for tracking the spatiotemporal distribution of important pathogenic forms. For example, multidrug-resistant DT104 has caused widespread infections in humans and animals in Europe and the United States since 1994 (12, 23), but it remains rare in Australia. In contrast, DT9, DT135, and DT170/108 are common in Australia (34), while DT9 is rare in Europe and the United States. However, knowledge of the evolutionary relationships between phage types is very limited, and the genetic basis for the variation in phage sensitivity remains largely unknown. The discriminatory power of phage typing is often inadequate to track community outbreaks, especially in settings when one particular phage type is dominant. In addition, phage types can change with a few genetic modifications, such as a gain or loss of mobile elements (2, 27, 50).Despite the extensive diversity of phage types in S. Typhimurium, population structure studies using multilocus enzyme electrophoresis (MLEE) showed that the majority of S. Typhimurium isolates are grouped together as a s...

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Section: Resultsmentioning

confidence: 99%

Genetic Relationships of Phage Types and Single Nucleotide Polymorphism Typing of Salmonella enterica Serovar Typhimurium

et al. 2012

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“…When the different systems and approaches are compared, limited differences in analytical performance are observed. Another MALDI-TOF MS system, focused on the analysis of nucleic acids, including PCR products and their derivatives, is offered by Sequenom (San Diego, CA) and uses the proprietary MassArray technology (27). The MassArray Analyzer systems combine molecular biology with MS.…”

Section: Bacterial Identification: Market Perspective and Manufacturersmentioning

confidence: 99%

Biomedical Mass Spectrometry in Today's and Tomorrow's Clinical Microbiology Laboratories

et al. 2012

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bClinical microbiology is a conservative laboratory exercise where base technologies introduced in the 19th century remained essentially unaltered. High-tech mass spectrometry (MS) has changed that. Within a few years following its adaptation to microbiological diagnostics, MS has been introduced, embraced, and broadly accepted by clinical microbiology laboratories throughout the world as an innovative tool for definitive bacterial species identification. Herein, we review the current state of the art with respect to this exciting new technology and discuss potential future applications.

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“…® system (Table 1) The MassARRAY iPLEX single-nucleotide polymorphism (SNP) typing platform uses and the MS technology coupled with single-base extension PCR to analyze amplicons of PCR for rapid and accurate molecular identification of microorganisms [63] . This system is commercialized by Sequenom TM (San Diego, CA, USA).…”

Section: Iplex Massarraymentioning

confidence: 99%

“…These two approaches provide a powerful tool in phylogenetic investigation, epidemiology (molecular typing) and surveillance of crossed transmission of bacteria [63,65,84] . An interesting example using the MassARRAY technology is the study performed by Syrmis et al [63] related with the genotyping of MRSA.…”

Section: Iplex Massarraymentioning

confidence: 99%

Mass spectrometry: a revolution in clinical microbiology?

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et al. 2012

Clinical Chemistry and Laboratory Medicine (CCLM)

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Recently, different bacteriological laboratory interventions that decrease reporting time have been developed. These promising new broad-based techniques have merit, based on their ability to identify rapidly many bacteria, organisms difficult to grow or newly emerging strains, as well as their capacity to track disease transmission. The benefit of rapid reporting of identification and/or resistance of bacteria can greatly impact patient outcomes, with an improvement in the use of antibiotics, in the reduction of the emergence of multidrug resistant bacteria and in mortality rates. Different techniques revolve around mass spectrometry (MS) technology: matrix-assisted laser desorption ionization time-offlight mass spectrometry (MALDI-TOF MS), PCR combined with electrospray ionization-mass spectrometry (PCR/ESI-MS), iPLEX MassArray system and other new evolutions combining different techniques. This report emphasizes the (r)evolution of these technologies in clinical microbiology.

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Comparison of a multiplexed MassARRAY system with real-time allele-specific PCR technology for genotyping of methicillin-resistant Staphylococcus aureus

Cited by 54 publications

References 23 publications

Genetic Relationships of Phage Types and Single Nucleotide Polymorphism Typing of Salmonella enterica Serovar Typhimurium

Genetic Relationships of Phage Types and Single Nucleotide Polymorphism Typing of Salmonella enterica Serovar Typhimurium

Biomedical Mass Spectrometry in Today's and Tomorrow's Clinical Microbiology Laboratories

Mass spectrometry: a revolution in clinical microbiology?

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