Comparison of a Multiplex Flow Cytometric Assay with Enzyme-Linked Immunosorbent Assay for Quantitation of Antibodies to Tetanus, Diphtheria, and
            <i>Haemophilus influenzae</i>
            Type b

Pickering, Jerry W.; Martins, Thomas B.; Schroder, M. C.; Hill, Harry R.

doi:10.1128/cdli.9.4.872-876.2002

Cited by 95 publications

(110 citation statements)

References 12 publications

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“…Optimally, a zero determination by one method should be recapitulated by another method's determination, provided that the sensitivity and specificity of the two methods are comparable. For Luminex methods and ELISA, this has been addressed previously (Biagini et al, 2004;Earley et al, 2002;Kellar et al, 2001;Pickering et al, 2002b;Prabhakar et al, 2002;Vignali, 2000). Very slight decreases in Pearson's correlation coefficients were seen with the above constraint; however, in no case did this result in a decrease below the threshold for a significant correlation.…”

Section: Resultsmentioning

confidence: 90%

Validation and comparison of luminex multiplex cytokine analysis kits with ELISA: Determinations of a panel of nine cytokines in clinical sample culture supernatants

duPont¹,

Wang

Wadhwa

et al. 2005

Journal of Reproductive Immunology

269

181

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Problem: Analyses of the expression pattern of multiple cytokines are frequently required for characterization of the status of the immune system as it pertains to Th type bias and intrinsic levels of inflammation. Classically, analysis of cytokine expression patterns has been performed by enzymelinked immunosorbent assays (ELISA) for each separate analyte. A new technology, Luminex MAP ® , facilitates the simultaneous evaluation of multiple immune mediators with advantages of higher throughput, smaller sample volume, and lower cost. Validation of this technology has been limited to small sample sets, limited use of clinical study specimens, and use of non-commercial reagents. * Corresponding author. Methods: Ninety-six specimens from women over the course of their respective pregnancies were evaluated for cytokine concentrations using commercially available ELISA kits and commercially available Luminex MAP ® kits according to the manufacturers' directions. Correlations between data sets were evaluated using Pearson's correlation coefficient (r).Results: Excellent correlations were demonstrated for IL-1␤, IL-4, IL-5, IL-6, IL-10, IFN ␥, and TNF ␣, in contrast to IL-12 p70 and IL-13. Conclusions: Luminex multiplex technology has distinct advantages and is a valid alternative method to ELISA for the evaluation of the majority of cytokines tested and for the characterization of immune system status.

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Section: Resultsmentioning

confidence: 90%

Validation and comparison of luminex multiplex cytokine analysis kits with ELISA: Determinations of a panel of nine cytokines in clinical sample culture supernatants

duPont¹,

Wang

Wadhwa

et al. 2005

Journal of Reproductive Immunology

269

181

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“…The MBA is at least as sensitive as ELISA, and as shown in this study, it was sufficiently sensitive to provide valuable sero-epidemiological data, including the pronounced agedependent downward shift in responses to the Giardia VSP antigens. [36][37][38][39][40][41] …”

Section: Estimated Mean Immunoglobulin G (Igg) Responses Tomentioning

confidence: 99%

Longitudinal Evaluation of Enteric Protozoa in Haitian Children by Stool Exam and Multiplex Serologic Assay

Moss

Priest

Hamlin³

et al. 2014

The American Journal of Tropical Medicine and Hygiene

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Abstract. Haitian children were monitored longitudinally in a filariasis study. Included were stool samples examined for Giardia intestinalis and Entamoeba histolytica cysts, and serum specimens analyzed for immunoglobulin G (IgG) responses to eight recombinant antigens from G. intestinalis (variant-specific surface protein [VSP1-VSP5]), E. histolytica (lectin adhesion molecule [LecA]), and Cryptosporidium parvum (17-and 27-kDa) using a multiplex bead assay. The IgG responses to VSP antigens peaked at 2 years of age and then diminished and were significantly lower (P 0.002) in children 4.5 years than in children 4.5 years. The IgG responses to Cryptosporidium tended to increase with age. The IgG responses to LecA and VSP antigens and the prevalence of stools positive for cysts were significantly higher (P 0.037 and P 0.035, respectively) in the rainy season than in the dry season. The multiplex bead assay provides a powerful tool for analyzing serologic responses to multiple pathogens.

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“…Antibody concentrations (IgG) against the Hib capsular polysaccharide (polyribosyl-ribitol phosphate (PRP)) were quantified using a fluorescent bead assay as described previously [9]. Briefly, PRP was conjugated to carboxylated microspheres (Luminex Corporation; Texas, United States) following bead activation (via a two-step carbodiimide reaction).…”

Section: Serum Samplesmentioning

confidence: 99%

Haemophilus influenzae serotype B (Hib) seroprevalence in England and Wales in 2009

Ladhani¹,

Ramsay²,

Flood³

et al. 2012

Eurosurveillance

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A national seroprevalence study was performed to determine the prevalence of Haemophilus influenzae type b (Hib) antibodies in England and Wales in 2009, when Hib disease incidence was the lowest ever recorded. A total of 2,693 anonymised residual sera from routine diagnostic testing submitted by participating National Health Service hospital laboratories were tested for Hib anti-polyribosyl-ribitol phosphate (PRP) IgG antibodies using a fluorescent bead assay. Median anti-PRP IgG concentrations were highest in toddlers aged 1-4 years (2.65 µg/ml), followed by children aged 5-9 years (1.95 µg/ml). Antibody concentrations were significantly lower after this age, but were still significantly higher among 10-19 year-olds (0.54 µg/ml) compared with adults aged >20 years (0.16 µg/ ml; p<0.0001). Half of the adults (51%) did not have Hib antibody concentrations ≥0.15 µg/ml, the level considered to confer short-term protection. Thus, the current excellent Hib control appears to be the result of high anti-PRP antibody concentrations in children aged up to 10 years, achieved through the various childhood vaccination campaigns offering booster immunisation. The lack of seroprotection in adults emphasises the importance of maintaining control of the disease and, most probably carriage, in children, therefore raising the question as to whether long-term routine boosting of either pre-school children or adolescents may be required.

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Comparison of a Multiplex Flow Cytometric Assay with Enzyme-Linked Immunosorbent Assay for Quantitation of Antibodies to Tetanus, Diphtheria, and Haemophilus influenzae Type b

Abstract: We developed a multiplexed indirect immunofluorescence assay for antibodies to

Cited by 95 publications

References 12 publications

Validation and comparison of luminex multiplex cytokine analysis kits with ELISA: Determinations of a panel of nine cytokines in clinical sample culture supernatants

Validation and comparison of luminex multiplex cytokine analysis kits with ELISA: Determinations of a panel of nine cytokines in clinical sample culture supernatants

Longitudinal Evaluation of Enteric Protozoa in Haitian Children by Stool Exam and Multiplex Serologic Assay

Haemophilus influenzae serotype B (Hib) seroprevalence in England and Wales in 2009

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