2006
DOI: 10.1111/j.1365-2672.2006.02889.x
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Comparison of a monoclonal antibody-based capture/enrichment sandwich enzyme linked immunosorbent assay with immunomagnetic bead separation for the detection of attachment effacement Escherichia coli O26 strains from cattle faeces

Abstract: Aims:  A monoclonal antibody (Mab 2F3)‐based sandwich enzyme linked immunosorbent assay (sELISA) format for the detection of Escherichia coli O26 that improves the sensitivity of the assay by combining enrichment with the capture stage has been developed. Culture of the enriched contents of wells before completion of the sELISA was compared with immunomagnetic bead separation (IMS) as a means of specific isolation of the target organism. Methods and Results:  Bovine faecal samples, c. 10% in buffered peptone w… Show more

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Cited by 3 publications
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“…ELISA detecting the presence of vtx 1 and vtx 2 have been used to detect VTEC (Paton and Paton 2003), and more recently, monoclonal antibodies against O26 surface antigens have been used in an ELISA format for the specific detection of E. coli O26 (Neely et al. 2004) and compared favourably with IMS (Finlay et al. 2006).…”
Section: Detection Of Escherichia Coli O26mentioning
confidence: 99%
“…ELISA detecting the presence of vtx 1 and vtx 2 have been used to detect VTEC (Paton and Paton 2003), and more recently, monoclonal antibodies against O26 surface antigens have been used in an ELISA format for the specific detection of E. coli O26 (Neely et al. 2004) and compared favourably with IMS (Finlay et al. 2006).…”
Section: Detection Of Escherichia Coli O26mentioning
confidence: 99%