Comparison of a Kinetic-Based Enzyme-Linked Immunosorbent Assay (KELISA) and Virus-Neutralization Test for Infectious Bursal Disease Virus. I. Quantitation of Antibody in White Leghorn Hens

Solano, W; Jj, Giambrone; Vs, Panangala

doi:10.2307/1590657

Cited by 13 publications

(4 citation statements)

References 16 publications

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“…The findings of this study demonstrated that a high level of mAb during primary immunization interfered with the take of IBDV vaccines. Similar finding was observed previously by Solano et al [15]. In their study, when high mAb (ELISA titer 16,384 on day 1) bearing chickens were vaccinated at 1 or 15 days of age with an intermediate live IBDV vaccine (Bursine-2), no detectable immediate primary antibody response was observed in chickens.…”

Section: Resultssupporting

confidence: 91%

Comparison of the Immunogenicity and Pathogenicity of a Genetically Engineered Infectious Bursal Disease Virus Vaccine and Two Commercial Live Vaccines in Chickens with Maternal Antibodies

Chowdhury,

Paul,

Badhy

et al. 2018

AJRAVS

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This work was carried out in collaboration between all authors. Author EUC designed the study, collected the samples, performed the statistical analysis, wrote the protocol and wrote the first draft of the manuscript. Author MRI supervised and managed the analyses of the study. Authors MRP and SCB assisted in sample collection, laboratory analyses, literature search, and writing report. All authors read and approved the final manuscript.

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Section: Resultssupporting

confidence: 91%

Comparison of the Immunogenicity and Pathogenicity of a Genetically Engineered Infectious Bursal Disease Virus Vaccine and Two Commercial Live Vaccines in Chickens with Maternal Antibodies

Chowdhury,

Paul,

Badhy

et al. 2018

AJRAVS

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“…Sera collected from the chickens immediately prier to, and 21 days after, challenge were tested for IBDV antibodies by an ELISA [31] using the PBG 98 IBDV strain grown in Vero cells as the antigen and a rabbit anti-chicken IgG phosphatase conjugate (Zymed) diluted 1:4000 to detect bound antibody. Sera were also tested for FPV antibodies by ELISA [23].…”

Section: Serologymentioning

confidence: 99%

A recombinant fowlpox virus that expresses the VP2 antigen of infectious bursal disease virus induces protection against mortality caused by the virus

Bayliss

Peters

Cook

et al. 1991

Archives of Virology

101

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The coding sequences of VP2 from a virulent strain, 52/70, of infectious bursal disease virus (IBDV) were excised from a cDNA clone and inserted into a fowlpox plasmid insertion vector. The resulting plasmid, pIBD 1, was used to construct a recombinant fowlpox virus, fpIBD 1, which expressed VP 2 as a beta-galactosidase fusion protein. Chickens vaccinated with fpIBD 1 at 1 and 14 days of age, were challenged at 28 days with either IBDV strain 52/70 or the highly virulent strain CS 89. These chickens were protected against mortality, but not against damage to the bursa of Fabricius. The protection achieved by the use of fpIBD 1 shows that VP 2 is a host protective antigen.

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“…The enzyme-linked immunosorbent assay (ELISA) is commonly used as a rapid and sensitive test for detection of IBDV antigen or antibody against IBDV (Marquardt et al, 1980;Howie andThorson, 1981 andSolano et al, 1985). ELISA is now being used for sero-profiling of chicken flocks and examination of the efficiency of vaccines (Solano et al, 1986). Studies of the molecular epidemiology of IBDV are important, and the DAS-ELISA could be an alternative technique for screening a large number of samples before testing (Tham et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Determination of Sensitivity and Specificity of in-House Sandwich Elisa for the Detection of Infectious Bursal Disease Viruses

Ali

Rahman

et al. 2012

Bangl. J. Vet. Med.

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The study was designed for the development of an In-House sandwich ELISA as a suitable serological method for the rapid detection of infectious bursal disease virus (IBDV). The test was also designed to compare and evaluate its sensitivity and specificity with other traditional methods used for the detection of IBDV from field outbreak cases prevalent among the poultry population of Bangladesh. To develop the In-House sandwich ELISA, hyper-immune serum was raised against live IBDV vaccine in rabbit which was used to coat each of the 96-well flat bottomed polystyrene microtitre plate whereas, hyper-immune sera raised in chickens against IBDV used as secondary antibody. The newly developed In-House sandwich ELISA was standardized by dispensing different dilutions (10-1 up to 10-4) of rabbit serum. Among them, the 10-2 dilution of serum showed most suitable reading for the detection of IBD virus and used to coat the plate to evaluate its sensitivity and specificity. Sensitivity test was done by different dilutions (10-0 to 10-4) of reference IBD virus. The virus dilution, 10-3 was the highest dilution having lowest capacity to bind with coated antibody of the ELISA plate which indicated that IBD viruses was absent in the dilutions of above 10-3. The cut-off value of negative control samples was determined as 0.937 which indicated titer of tested samples >0.937 was positive and <0.937 was negative. Specificity test was performed using different known viruses (IBDV and NDV) using different dilutions (10-1 up to 10-4). Only the IBDV showed positive result which indicated high specificity of newly developed ELISA plate. A total of 26 samples (feces, cloacal swab, spleen and bursa) from control group, experimental and natural IBDV outbreaks were used as field viral antigen for the evaluation of sensitivity and specificity of the newly developed In-House sandwich ELISA. In case of experimental infection, 5 (62.5%) of 8 feces sample but none of cloacal swab were positive for IBDV whereas, all bursa and spleen samples were positive by both InHouse sandwich ELISA and AGIDT. In case of natural outbreak cases, 6 of 6 bursal samples and 4 of 6 spleen samples were positive by In-House sandwich ELISA whereas, AGIDT detected all bursal and 3 spleen samples. No virus was detected from the samples of control group. The result showed 92.85% specificity of the developed sandwich ELISA for detection of IBDV with AGIDT which indicated that the developed ELISA is a sensitive, specific, cost effective and reliable tool for the detection of IBDV antigen from a large number of field samples.DOI = http://dx.doi.org/10.3329/bjvm.v8i2.11185 Bangl. J. Vet. Med. (2010). 8 (2) : 97-106

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Comparison of a Kinetic-Based Enzyme-Linked Immunosorbent Assay (KELISA) and Virus-Neutralization Test for Infectious Bursal Disease Virus. I. Quantitation of Antibody in White Leghorn Hens

Cited by 13 publications

References 16 publications

Comparison of the Immunogenicity and Pathogenicity of a Genetically Engineered Infectious Bursal Disease Virus Vaccine and Two Commercial Live Vaccines in Chickens with Maternal Antibodies

Comparison of the Immunogenicity and Pathogenicity of a Genetically Engineered Infectious Bursal Disease Virus Vaccine and Two Commercial Live Vaccines in Chickens with Maternal Antibodies

A recombinant fowlpox virus that expresses the VP2 antigen of infectious bursal disease virus induces protection against mortality caused by the virus

Determination of Sensitivity and Specificity of in-House Sandwich Elisa for the Detection of Infectious Bursal Disease Viruses

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