Comparison of a Homogeneous Assay With a Precipitation Method for the Measurement of HDL Cholesterol in Diabetic Patients

Jensen, Tonny; Truong, Quan; Frandsen, M.; Dinesen, Bo; Stender, Steen

doi:10.2337/diacare.25.11.1914

Cited by 12 publications

(5 citation statements)

References 14 publications

(8 reference statements)

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“…In previous studies, conventional precipitation technique was used for measuring HDL-C. It has been demonstrated that the precipitation method for HDL-C correlated well with the ultracentrifugation method but that the precipitation method exhibited a negative bias for HDL-C (Jensen et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of the metabolic syndrome among Turkish adults

Oğuz²,

et al. 2006

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Objective: To determine prevalence of the metabolic syndrome (MS) in a sample representing Turkish population using United States Adult Treatment Panel-3 guidelines. Design: The study included random samples from both urban and rural populations in the seven geographical regions of Turkey. The population for this analysis were 2108 men (1372 in urban and 736 in rural areas) and 2151 women (1423 in urban and 728 in rural areas) with a mean age of 40.9714.9 years (range 20-90). Results: The prevalence of the MS diagnosed using the Adult Treatment Panel III criteria was 33.9% (1442 of 4259) and differed significantly in men (28%) and women (39.6%). The prevalence of syndrome increased with age in men, from 10.7% in subjects aged 20-29 years to 49% in those aged over 70 years. The prevalence increased with age in women, from 9.6% in subjects aged 20-29 years to 74.6% in those aged 60-69 years, and decreased to 68.6% in those over 70 years of age. The prevalence of the syndrome was similar in urban (33.8%) and rural (33.9%) population. We found 26.8, 26.4, 19.3, 10.9 and 3.6% of the population had at least 1, 2, 3, 4 or 5 components, respectively. We found 57.2, 32.3 and 10.6% of the subjects with MS had 3, 4 and 5 components, respectively.

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Section: Discussionmentioning

confidence: 99%

Prevalence of the metabolic syndrome among Turkish adults

Oğuz²,

et al. 2006

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“…Plasma cholesterol levels were measured with a cholesterol assay kit from BioVision Inc (Mountain View, CA). HDL-cholesterol level was determined by phosphotungstic acid and magnesium chloride precipitation method46.…”

Section: Methodsmentioning

confidence: 99%

Bitter melon extract attenuating hepatic steatosis may be mediated by FGF21 and AMPK/Sirt1 signaling in mice

Zhang

Ebersole

et al. 2013

Sci Rep

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We sought to evaluate the effects of Momordica charantia (bitter melon, BM) extract on insulin sensitivity, NAFLD, hepatic FGF21 and AMPK signaling in mice fed a high-fat diet. Male C57/B6 mice were randomly divided into HFD and HFD supplementation with BM for 12 week. Body weight, plasma glucose, FGF21 and insulin levels, hepatic FGF21 and AMPK signaling proteins were measured. The results showed that plasma FGF21 and insulin concentrations were significantly decreased and hepatic FGF21 content was significantly down-regulated, while FGF receptors 1, 3 and 4 (FGFR1, FGFR3 and FGFR4) were greatly up-regulated in BM group compared to the HFD group (P < 0.05 and P < 0.01). BM also significantly increased hepatic AMPK p, AMPK α1 AMPK α2 and Sirt1 content compared to the HFD mice. We, for the first time, demonstrated that BM extract attenuated hepatic steatosis in mice by enhancing hepatic FGF21 and AMPK/Sirt1 signaling.

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“…Plasma glucose (glucose CII‐test), cholesterol (cholesterol C‐test), triglyceride (triglyceride C‐test) and phospholipid (phospholipid C‐test) were determined using an assay kit (Wako Pure Chemical Industries, Osaka, Japan). High‐density lipoprotein cholesterol was measured by phosphotungstic acid and magnesium chloride precipitation [30]. The precipitating reagent consisted of 0.55 m m phosphotungstic acid and 25 m m magnesium chloride.…”

Section: Methodsmentioning

confidence: 99%

Fatty acids increase the circulating levels of oxidative stress factors in mice with diet‐induced obesity via redox changes of albumin

et al. 2007

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Metabolic syndrome is characterized by a constellation of multiple risk factors, such as dyslipidemia, hyperglycemia, hypertension, and abdominal obesity. Several studies have focused on the role of oxidative stress in metabolic syndrome [1][2][3][4][5][6]. For example, oxidative stress markers, such as thiobarbituric acid reactive substances (TBARS), an index of lipid peroxidation, 8-hydroxy-2¢-deoxyguanosine, a biomarker of oxidative DNA damage, and oxidative modification of low-density lipoprotein, increased in the plasma of a rat model of metabolic syndrome [6]. Plasma concentrations of free fatty acids are also increased in metabolic syndrome, and might be involved in the pathogenesis of skeletal muscle insulin resistance [7,8]. Increased fatty acids resulted in cellular damage via the induction of oxidative stress [9,10]. In skeletal muscle cells, palmitate-induced mitochondrial DNA damage and cytotoxicity were caused by the overproduction of peroxynitrite [9]. Zhang et al. demonstrated that fatty acids enhanced monocyte adhesion to endothelial cells in vitro and that the process was mediated through the increased generation of reactive oxygen species and the enhanced expression of the integrin CD11b [10]. Plasma concentrations of free fatty acids are increased in metabolic syndrome, and the increased fatty acids may cause cellular damage via the induction of oxidative stress. The present study was designed to determine whether the increase in fatty acids can modify the free sulfhydryl group in position 34 of albumin (Cys34) and enhance the redox-cycling activity of the copper-albumin complex in high-fat diet-induced obese mice. The mice were fed with commercial normal diet or high-fat diet and water ad libitum for 3 months. The high-fat diet-fed mice developed obesity, hyperlipemia, and hyperglycemia. The plasma fatty acid ⁄ albumin ratio also significantly increased in high-fat diet-fed mice. The increased fatty acid ⁄ albumin ratio was associated with conformational changes in albumin and the oxidation of sulfhydryl groups. Moreover, an ascorbic acid radical, an index of redox-cycling activity of the copper-albumin complex, was detected only in the plasma from obese mice, whereas the plasma concentrations of ascorbic acid were not altered. Plasma thiobarbituric acid reactive substances were significantly increased in the high-fat diet group. These results indicate that the increased plasma fatty acids in the high-fat diet group resulted in the activated redox cycling of the copper-albumin complex and excessive lipid peroxidation.

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Comparison of a Homogeneous Assay With a Precipitation Method for the Measurement of HDL Cholesterol in Diabetic Patients

Cited by 12 publications

References 14 publications

Prevalence of the metabolic syndrome among Turkish adults

Prevalence of the metabolic syndrome among Turkish adults

Bitter melon extract attenuating hepatic steatosis may be mediated by FGF21 and AMPK/Sirt1 signaling in mice

Fatty acids increase the circulating levels of oxidative stress factors in mice with diet‐induced obesity via redox changes of albumin

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