Comparison of a compatible and an incompatible pepper-tobamovirus interaction by biochemical and non-invasive techniques: Chlorophyll a fluorescence, isothermal calorimetry and FT-Raman spectroscopy

Rys, Magdalena; Juhász, Csilla; Surówka, Ewa; Janeczko, Anna; Saja, Diana; Tóbiás, István; Skoczowski, Andrzej; Barna, B.; Gullner, Gábor

doi:10.1016/j.plaphy.2014.08.013

Cited by 45 publications

(35 citation statements)

References 39 publications

(56 reference statements)

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“…Following ObPV inoculation the first visible symptoms (hypersensitive necrotic lesions) appeared 72 hpi, in accordance with earlier studies (Rys et al 2014). Pretreatment with 100 nM 24-epi-BR significantly alleviated the visible symptoms of ObPV inoculation (Fig.…”

Section: Symptoms Of Virus Inoculationssupporting

confidence: 91%

“…Analysis of chlorophyll (Chl) fluorescence induction curves (slow and fast Chl a fluorescence kinetic) is a very popular method to estimate the effectiveness of photosystems (Papageorgiou et al 2007). This method has been already used during studies devoted to virus-plant interactions (Baron et al 1995, Gonçalves et al 2005, Rys et al 2014. Methods allowing the measurement of leaf reflectance are less popular but also useful for the study of virus-plant interactions, for example for an early detection of viral infection in tomato (Krezhova et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, by using noninvasive methods to characterize the efficiency of photosynthesis, we may detect metabolic disorders before the appearance of visible symptoms of virus infection, which could be of practical importance. (Tóbiás et al 1989, Rys et al 2014. Both viral inoculations were made with cellit as an abrasive.…”

Section: Introductionmentioning

confidence: 99%

“…(5) Plants were sprayed with 100 nM 24-epi-BR two days before PMMoV inoculation. Virus inoculations were carried out on the fifth and sixth true leaves as described earlier (Rys et al 2014).…”

Section: Introductionmentioning

confidence: 99%

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Comparative studies of compatible and incompatible pepper-Tobamovirus interactions and the evaluation of effects of 24-epibrassinolide

Janeczko¹,

Dziurka²,

Gullner³

et al. 2018

Photosynt.

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The aim of study was to gain a deeper knowledge about local and systemic changes in photosynthetic processes and sugar production of pepper infected by Obuda pepper virus (ObPV) and Pepper mild mottle virus (PMMoV). PSII efficiency, reflectance, and gas exchange were measured 48 and/or 72 h after inoculation (hpi). Sugar accumulation was checked 72 hpi and 20 d after inoculation (as a systemic response). Inoculation of leaves with ObPV led to appearance of hypersensitive necrotic lesions (incompatible interaction), while PMMoV caused no visible symptoms (compatible interaction). ObPV (but not PMMoV) lowered F v /F m (from 0.827 to 0.148 at 72 hpi). Net photosynthesis decreased in ObPV-infected leaves. In ObPV-inoculated leaves, the accumulation of glucose, fructose, and glucose-6-phosphate was accompanied with lowered sucrose, maltoheptose, nystose, and trehalose contents. PMMoV inoculation increased the contents of glucose, maltose, and raffinose in the inoculated leaves, while glucose-6-phosphate accummulated in upper leaves.Additional key words: brassinosteroids; CO2 assimilation; systemic virus response; water band index; xanthophyll cycle.

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Section: Symptoms Of Virus Inoculationssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparative studies of compatible and incompatible pepper-Tobamovirus interactions and the evaluation of effects of 24-epibrassinolide

Janeczko¹,

Dziurka²,

Gullner³

et al. 2018

Photosynt.

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“…Rys et al [43] compared a compatible (PMMoV) with an incompatible [Obuda pepper virus (ObPV)] peppertobamovirus interaction by Chl-FI and other non-invasive techniques. After the appearance of necrotic lesions, due to inoculation with ObPV, strong inhibition of photochemical energy conversion was observed in these areas; however, leaf tissues adjacent to these inactive areas showed elevated Φ PSII and F V /F M values.…”

Section: Chlorophyll Fluorescence Imaging To Monitor Plants Infected mentioning

confidence: 99%

Picturing pathogen infection in plants

Barón

Pineda

Pérez-Bueno

2016

Zeitschrift Für Naturforschung C

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Several imaging techniques have provided valuable tools to evaluate the impact of biotic stress on host plants. The use of these techniques enables the study of plant-pathogen interactions by analysing the spatial and temporal heterogeneity of foliar metabolism during pathogenesis. In this work we review the use of imaging techniques based on chlorophyll fluorescence, multicolour fluorescence and thermography for the study of virus, bacteria and fungi-infected plants. These studies have revealed the impact of pathogen challenge on photosynthetic performance, secondary metabolism, as well as leaf transpiration as a promising tool for field and greenhouse management of diseases. Images of standard chlorophyll fluorescence (Chl-F) parameters obtained during Chl-F induction kinetics related to photochemical processes and those involved in energy dissipation, could be good stress indicators to monitor pathogenesis. Changes on UV-induced blue (F440) and green fluorescence (F520) measured by multicolour fluorescence imaging in pathogen-challenged plants seem to be related with the up-regulation of the plant secondary metabolism and with an increase in phenolic compounds involved in plant defence, such as scopoletin, chlorogenic or ferulic acids. Thermal imaging visualizes the leaf transpiration map during pathogenesis and emphasizes the key role of stomata on innate plant immunity. Using several imaging techniques in parallel could allow obtaining disease signatures for a specific pathogen. These techniques have also turned out to be very useful for presymptomatic pathogen detection, and powerful non-destructive tools for precision agriculture. Their applicability at lab-scale, in the field by remote sensing, and in high-throughput plant phenotyping, makes them particularly useful. Thermal sensors are widely used in crop fields to detect early changes in leaf transpiration induced by both air-borne and soil-borne pathogens. The limitations of measuring photosynthesis by Chl-F at the canopy level are being solved, while the use of multispectral fluorescence imaging is very challenging due to the type of light excitation that is used.Keywords: biotic stress; chlorophyll fluorescence imaging; multicolor fluorescence imaging; plant pathogens; thermography. Dedication: This work is dedicated to the memory of Professor Peter Böger. He will always bring to my heart (M. B.) the best memories of my postdoc at Lake Constance.

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Raman spectroscopy‐based diagnostics of water deficit and salinity stresses in two accessions of peanut

Morey

Farber

McCutchen³

et al. 2021

Plant Direct

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Water deficit and salinity are two major abiotic stresses that have tremendous effect on crop yield worldwide. Timely identification of these stresses can help limit associated yield loss. Confirmatory detection and identification of water deficit stress can also enable proper irrigation management. Traditionally, unmanned aerial vehicle (UAV)‐based imaging and satellite‐based imaging, together with visual field observation, are used for diagnostics of such stresses. However, these approaches can only detect salinity and water deficit stress at the symptomatic stage. Raman spectroscopy (RS) is a noninvasive and nondestructive technique that can identify and detect plant biotic and abiotic stress. In this study, we investigated accuracy of Raman‐based diagnostics of water deficit and salinity stresses on two greenhouse‐grown peanut accessions: tolerant and susceptible to water deficit. Plants were grown for 76 days prior to application of the water deficit and salinity stresses. Water deficit treatments received no irrigation for 5 days, and salinity treatments received 1.0 L of 240‐mM salt water per day for the duration of 5‐day sampling. Every day after the stress was imposed, plant leaves were collected and immediately analyzed by a hand‐held Raman spectrometer. RS and chemometrics could identify control and stressed (either water deficit or salinity) susceptible plants with 95% and 80% accuracy just 1 day after treatment. Water deficit and salinity stressed plants could be differentiated from each other with 87% and 86% accuracy, respectively. In the tolerant accessions at the same timepoint, the identification accuracies were 66%, 65%, 67%, and 69% for control, combined stresses, water deficit, and salinity stresses, respectively. The high selectivity and specificity for presymptomatic identification of abiotic stresses in the susceptible line provide evidence for the potential of Raman‐based surveillance in commercial‐scale agriculture and digital farming.

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Comparison of a compatible and an incompatible pepper-tobamovirus interaction by biochemical and non-invasive techniques: Chlorophyll a fluorescence, isothermal calorimetry and FT-Raman spectroscopy

Cited by 45 publications

References 39 publications

Comparative studies of compatible and incompatible pepper-Tobamovirus interactions and the evaluation of effects of 24-epibrassinolide

Comparative studies of compatible and incompatible pepper-Tobamovirus interactions and the evaluation of effects of 24-epibrassinolide

Picturing pathogen infection in plants

Raman spectroscopy‐based diagnostics of water deficit and salinity stresses in two accessions of peanut

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