Comparison of a commercial ELISA with an indirect fluorescent antibody test to detect antibodies to<i>Lawsonia intracellularis</i>in experimentally challenged pigs

Collins, A. M.; Gonsalves, Jocelyn R.; Fell, Shayne A.; Barchia, I.

doi:10.1111/j.1751-0813.2011.00865.x

Cited by 7 publications

(5 citation statements)

References 8 publications

(8 reference statements)

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“…Specificity and sensitivity of the test employed in this study were according to the previous report [13]. …”

Section: Resultsmentioning

confidence: 99%

Seroprevalence of porcine proliferative enteropathy among wild boars in the Republic of Korea

Yeh

2014

BMC Vet Res

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BackgroundThe importance of the wild boar as a reservoir of Lawsonia intracellularis was assessed by investigating the seroprevalence of this pathogen among wild boars in the Republic of Korea. The extent of exposure to L. intracellularis among wild boars (Sus scrofa coreanus) was monitored by a country-wide serological survey using an immunoperoxidase monolayer assay.ResultsIn this study, antibodies to L. intracellularis were observed in 165 of 716 clinically healthy wild boars tested. The overall apparent prevalence calculated directly from the sample and the true prevalence calculated based on the accuracy of the test method were 23.0% (95% confidence interval: 20.0-26.3%) and 25.6% (95% confidence interval: 23.9-27.2%), respectively. Serologically positive animals were found in all the tested provinces.ConclusionsOur results confirm that L. intracellularis is present in the wild boar population worldwide, even in Far East Asia. Despite the high seroprevalence shown in wild boars, further studies are warranted to evaluate their potential as a reservoir species because seroprevalence does not prove ongoing infection nor shedding of the bacteria in amounts sufficient to infect other animals. It should also be determined whether the wild boar, like the domestic pig, is a natural host of L. intracellularis.

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“…Specificity and sensitivity of the test employed in this study were according to the previous report [13]. …”

Section: Resultsmentioning

confidence: 99%

Seroprevalence of porcine proliferative enteropathy among wild boars in the Republic of Korea

Yeh

2014

BMC Vet Res

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“…Serum samples from all pigs were tested for antibodies against L. intracellularis (SVANOVIR L.intracellularis/Ileitis-Ab, Boehringer Ingelheim Svanova, Sweden, successor of the bioScreen Ileitis Antibody ELISA with sensitivity (Se) ranging from 72 to 96.5% and specificity (Sp) from 83 to 100%; [ 17 – 19 ]), M. hyopneumoniae ( Mycoplasma hyopneumoniae ELISA, OXOID Ltd., UK; formely DAKO ELISA; with Sp = 100% and Se ranging from 49% to 100% according to experimental trials [ 20 – 23 ]) and PCV2 (SERELISA® PCV2 Ab Mono Blocking, Synbiotics Europe, France, Se = 86% and Sp = 85% [ 24 ]). A serum sample was considered positive for L. intracellularis antibodies if the percentage inhibition was ≥30% [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

Factors associated with the growing-finishing performances of swine herds: an exploratory study on serological and herd level indicators

et al. 2018

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BackgroundGrowing and finishing performances of pigs strongly influence farm efficiency and profitability. The performances of the pigs rely on the herd health status and also on several non-infectious factors. Many recommendations for the improvement of the technical performances of a herd are based on the results of studies assessing the effect of one or a limited number of infections or environmental factors. Few studies investigated jointly the influence of both type of factors on swine herd performances. This work aimed at identifying infectious and non-infectious factors associated with the growing and finishing performances of 41 French swine herds.ResultsTwo groups of herds were identified using a clustering analysis: a cluster of 24 herds with the highest technical performance values (mean average daily gain = 781.1 g/day +/− 26.3; mean feed conversion ratio = 2.5 kg/kg +/− 0.1; mean mortality rate = 4.1% +/− 0.9; and mean carcass slaughter weight = 121.2 kg +/− 5.2) and a cluster of 17 herds with the lowest performance values (mean average daily gain =715.8 g/day +/− 26.5; mean feed conversion ratio = 2.6 kg/kg +/− 0.1; mean mortality rate = 6.8% +/− 2.0; and mean carcass slaughter weight = 117.7 kg +/− 3.6). Multiple correspondence analysis was used to identify factors associated with the level of technical performance. Infection with the porcine reproductive and respiratory syndrome virus and the porcine circovirus type 2 were infectious factors associated with the cluster having the lowest performance values. This cluster also featured farrow-to-finish type herds, a short interval between successive batches of pigs (≤3 weeks) and mixing of pigs from different batches in the growing or/and finishing steps. Inconsistency between nursery and fattening building management was another factor associated with the low-performance cluster. The odds of a herd showing low growing-finishing performance was significantly increased when infected by PRRS virus in the growing-finishing steps (OR = 8.8, 95% confidence interval [95% CI]: 1.8–41.7) and belonging to a farrow-to-finish type herd (OR = 5.1, 95% CI = 1.1–23.8).ConclusionsHerd management and viral infections significantly influenced the performance levels of the swine herds included in this study.Electronic supplementary materialThe online version of this article (10.1186/s40813-018-0082-9) contains supplementary material, which is available to authorized users.

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“…Several ELISAs have been developed for the detection of L. intracellularis antibodies in serum. 16 , 44 , 54 , 61 Most are competitive ELISAs, the major advantage of which is their ability to selectively detect the antibody recognized by the plated antigen, even in crude or impure samples. To date, only one commercial kit is available (Svanovir L. intracellularis / Ileitis -Ab; Table 1 ).…”

Section: Detection Of Antibodies Against L Intracellularismentioning

confidence: 99%

Review of methods for the detection of Lawsonia intracellularis infection in pigs

et al. 2021

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Lawsonia intracellularis is an obligate intracellular bacterium associated with enteric disease in pigs. Clinical signs include weight loss, diarrhea, and, in some cases, sudden death. The hallmark lesion is the thickening of the intestinal mucosa caused by increased epithelial cell replication, known as proliferative enteropathy. The immune response to L. intracellularis is not well defined, and detection of the infection, especially in the early stages, is still a significant challenge. We review here the main approaches used to identify this important but poorly understood pathogen. Detection of L. intracellularis infection as the cause of clinical disease is confounded by the high prevalence of the pathogen in many countries and that several other pathogens can produce similar clinical signs. A single L. intracellularis–specific ELISA and several amplification assays are available commercially to aid detection and surveillance, although histopathology remains the primary way to reach a conclusive diagnosis. There are major gaps in our understanding of L. intracellularis pathogenesis, especially how the host responds to infection and the factors that drive infection toward different clinical outcomes. Knowledge of pathogenesis will increase the predictive value of antemortem tests to guide appropriate interventions, including identification and treatment of subclinically affected pigs in the early stages of disease, given that this important manifestation reduces pig productivity and contributes to the economic burden of L. intracellularis worldwide.

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Comparison of a commercial ELISA with an indirect fluorescent antibody test to detect antibodies toLawsonia intracellularisin experimentally challenged pigs

Abstract: ELISA can be successfully used to monitor L. intracellularis infection in pigs.

Cited by 7 publications

References 8 publications

Seroprevalence of porcine proliferative enteropathy among wild boars in the Republic of Korea

Seroprevalence of porcine proliferative enteropathy among wild boars in the Republic of Korea

Factors associated with the growing-finishing performances of swine herds: an exploratory study on serological and herd level indicators

Review of methods for the detection of Lawsonia intracellularis infection in pigs

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