Comparison of a Colorimetric and a High Performance Liquid Chromatography Method for the Determination of Fructan in Pasture Grasses for Horses

Longland, A. C.; Harris, Patricia A.

doi:10.1016/j.jevs.2009.04.080

Cited by 2 publications

(1 citation statement)

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“…High NSC (HC; Italian Rye, Lolium multiflorum , 17.1% NSC), low NSC (LC; Blue Grama, Boutelous gracilis , 4.4% NSC) and medium NSC (MC; mixed grass, MN 10.7% NSC; KY 10.6% NSC) hays were identified by estimating NSC (WSC plus starch) and fructan (WSC–ethanol‐soluble carbohydrate) (Equi‐Analytical Laboratories, Ithaca, NY, USA; Table 1). This method has been previously used to provide a rough estimate of fructan content for fresh ryegrass, but proved to be less accurate when compared with high‐performance liquid chromatography (HPLC) analysis for ryegrass hay (Longland and Harris, 2009a). Fructan and starch contents were subsequently assayed by enzymatic technique (MegaZyme; NP Analytical Laboratories, St Louis, MO, USA), which may underestimate fructan content in timothy hay (Longland and Harris, 2009b).…”

Section: Methodsmentioning

confidence: 99%

Glycaemic and insulinaemic responses to feeding hay with different non‐structural carbohydrate content in control and polysaccharide storage myopathy‐affected horses

Borgia

Valberg

McCue

et al. 2010

Animal Physiology Nutrition

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The aim of this study was to determine whether the glycaemic/insulinaemic responses to hay with non-structural carbohydrate (NSC, soluble carbohydrate) of 17% (HC), 10% (MC) or 4% (LC) differs in control horses and whether these responses differ between control and horses with polysaccharide storage myopathy (PSSM). Five clinically normal control horses and seven PSSM horses, all unfit and of Quarter Horse breeding (age 9.4 ± 3.4 years, body condition score range: 4.5-6). A crossover design compared the HC and LC hay, with horses randomly assigned to hay type for 5 days, and all horses fed the MC hay during washout, after which the diets were switched. Horses were fed 1.5% BW (as fed) divided into 2 feeding per day, no grain. On morning of the fifth day of each block (seventh day for washout), horses were given 0.5% BW in hay, blood was drawn before and every 30 min for 5 h after feeding, and the rate of intake was measured. Whole blood glucose and plasma insulin were measured. The intake rate was significantly higher for HC. In control horses, the insulin area under the curve (6891.7 ± 3524.2 HC vs. 1185.4 ± 530.2 LC) was significantly higher than LC. Polysaccharide storage myopathy horses had significantly higher glycaemic and insulinaemic responses to HC vs. LC, however; the magnitude of insulin response was lower and glucose response higher in PSSM vs. control horses. Results suggest that insulin responses can differ significantly with the NSC content of hay. Feeding hay with 17% NSC produces elevations in insulin that could be detrimental for PSSM horses.

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Section: Methodsmentioning

confidence: 99%