Comparison of 5-Fluorouracil-related Gene Expression Levels Between Adenocarcinomas and Squamous Cell Carcinomas of the Lung

Ishihama, Hiromi; Chida, Masayuki; Araki, Osamu; Karube, Yoko; Seki, Norio; Tamura, Motohiko; Umezu, Hideo; Honma, Koichi; Masawa, Nobuhide; Miyoshi, Shinichiro

doi:10.1093/jjco/hyn121

Cited by 25 publications

(17 citation statements)

References 5 publications

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“…Anti-metabolic drugs, such as pemetrexed, gemcitabine, and 5-fluorouracil and its derivatives, are widely used as cancer chemotherapy agents, and their effects include inhibition of TS, as well as incorporation of its metabolites into RNA and DNA. Lower TS activity is thought to be correlated with greater sensitivity to anti-metabolic drugs [8, 9]. The present findings showed that gene expression levels of TS mRNA in PPC were significantly higher than in AC, whereas those of DPD and TP mRNA in PPC were similar to those in SCC, in contrast to AC.…”

Section: Discussionsupporting

confidence: 41%

“…Those from patients who received preoperative induction therapy including chemotherapy or chemoradiotherapy were excluded. To compare with other types of lung cancer, 40 stage-matched adenocarcinoma (AC) and 35 squamous cell carcinoma (SCC) cases in our database [8] were also examined. Dokkyo Medical University Hospital Ethics Committee approved this retrospective study (#24035) and waived the need for patient consent for analysis of the results.…”

Section: Methodsmentioning

confidence: 99%

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Tumor-related gene expression levels in pulmonary pleomorphic carcinoma

Oyaizu

Matsumura

Kobayashi

et al. 2015

J Cardiothorac Surg

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BackgroundPulmonary pleomorphic carcinoma (PPC) is a rare type of non-small-cell lung cancer that belongs to the family of sarcomatoid carcinomas and is associated with poor prognosis. We investigated the expressions of tumor-related genes in resected PPC specimens.MethodsSpecimens resected from patients with PPC from July 2006 through April 2012 were investigated. Tumor segments were collected from the specimens by micro-dissection to extract mRNA, then RT-PCR was performed according to Dannenberg’s tumor profile method for semi-quantitation of tumor-related gene mRNA. To compare with other types of lung cancer, data from stage-matched adenocarcinoma (AC) and squamous cell carcinoma (SCC) cases in our database were also examined.ResultsThe gene expression levels of thymidylate synthase were significantly higher in PPC and SCC as compared to the AC specimens (p < 0.001). The levels of dihydropyrimidine dehydrogenase and thymidine phosphorylase mRNA in PPC showed a similar tendency to those in SCC, in contrast to AC. Furthermore, the expression level of excision repair cross-complementation group 1 mRNA in PPC specimens was similar to that reported in NSCLC, while the level of vascular endothelial growth factor (VEGF) expression was higher as compared to that reported for colorectal cancer.ConclusionsAlthough gene expression of tumor cannot be directly correlated to its sensitivity for anti-cancer drugs, it is likely that PPC tumors are not sensitive to anti-metabolic drugs. Anti-VEGF therapy may be a candidate for PPC, while cisplatin also remains a viable option.

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Section: Discussionsupporting

confidence: 41%

Section: Methodsmentioning

confidence: 99%

Tumor-related gene expression levels in pulmonary pleomorphic carcinoma

Oyaizu

Matsumura

Kobayashi

et al. 2015

J Cardiothorac Surg

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show abstract

“…The expression of TS and OPRT also has been described to significantly lower in adenocarcinoma than squamous cell carcinoma of the lung. Several investigators have documented the relationship between TS expression and 5-FUbased chemotherapy in squamous cell carcinoma [23]. In patients with oral squamous cell carcinoma, the response to chemotherapy was higher in the group with low TS activity compared with high TS activity [24].…”

Section: Discussionmentioning

confidence: 99%

Expression of thymidylate synthase, orotate phosphoribosyltransferase and dihydropyrimidine dehydrogenase in thymic epithelial tumors

et al. 2011

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“…Further investigations will therefore be necessary to examine the method of detecting TS expression [25,26], validation, the meaning of histological differences [27], and efficacy of adjuvant chemotherapy [28,29] in a prospective trial.…”

Section: Discussionmentioning

confidence: 99%