[a] 1IntroductionProstate cancer accounts for 10 %o fa ll deaths from cancer [1,2] and the prostate specific antigen (PSA) is the best serumm arkerc urrently available for the preoperative diagnosis and screening for this cancer. PSA in serum circulates under two forms (free PSA(fPSA) [3] and complexed,b eing the predominanto ne the complex with a 1-antichymotripsin (PSA-ACT) [4].I np rostate cancer diagnosis,t he sum of both forms noted total PSA (tPSA) is used to determine somec ut off.Avalue of tPSA above 10.0 ng/mL is regardeda sp ositive and indicates high probability of prostate cancer;b elow 4.0 ng/mL the result is considered negativea nd indicatesl ow probability of prostate cancer;b etween 4.0 ng/mL and 10 ng/mLt he result is in the so-called "grey zone" [5].T he quantitative detection of PSA has been carried out using optical methods including fluorescent labeling [6,7],s urface enhanced Raman scattering (SERS) [8],s urface Plasmon resonance (SPR) [9],o relectrochemicalm ethods [10][11][12].Point-of-care testing (POCT)i sn ecessary and thus traditional techniques utilizing highly expensive, immovable analytical equipment such as gas Chromatography-Mass Spectrometers are not feasible for sensing outside the laboratories [13-17] Screen-printing is aw ell establish technique for the reliable fabrication of robust biosensors, due to its low cost and applicability to mass production. Screen-printed electrodes (SPEs) in particular can combine ease of use and portability with simple,i nexpensive screen-printedt echniques and nowadays it can be found different designsoft his device [18][19][20][21][22].Despite manye fforts have been made in developing sensors for PSA detection, many challenges still need to be addressed such as their miniaturization, simplification of the fabrication steps and cost reduction.Magnetic beads (MBs)are used in bioassay with the capability to increase surface area to improve sensitivity [23].M ore important, MBs are easy to manipulate with the help of magnet and make possible the biological reaction events could be performed awayf rom the electrode surface [24],e ffectively reducing the complexity and time required for sensing application. Their use minimizes matrix effect due to washing and separation procedures and, since the beads are in suspension, allows faster assay kineticst ob ea chieved [25].M oreoverM Bs can be easily functionalized with differentl inkageg roups uch as streptavidin [26][27][28],t osyl groups [29][30][31][32], amino groups [33][34][35],a ntibodies or proteins [36][37][38][39].T he only problem represented by the use of the MBsi st hat the recognition elementisnot in direct contact with the surface electrode. To avoid this and to achieve greater sensitivity,i th as been proposed the use of magnetized electrode surface through either the inclusion in the electrode material of magneticp articles [40] or through the placement of small magnets below the surface of the working electrode [41,42].T his allows concentrating the MBs on the electrode surface for t...