Comparison of 2 molecular assays and a serologic test in diagnosing Mycoplasma pneumoniae infection in paediatrics patients

Blanco, Silvia; Fuenzalida, Loreto; Bas, Albert; Prat, Cristina; Ramírez, Aída; Matas, Lurdes; Corrêa-Oliveira, Rodrigo; Ausina, V.

doi:10.1016/j.diagmicrobio.2011.08.022

Cited by 6 publications

(5 citation statements)

References 15 publications

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“…With regard to the sensitivity obtained for in-house mPCR and commercial kits using the quadrupling of titers in paired serology as the gold standard, this was lower than previous studies [ 15 , 21 ], in which the sensitivity ranged from 66.7 to 97.3 %. Even sensitivities and specificities of up to 100 % have been described when the gold standard used was a PCR monoplex assay and the study population was comprised solely of positive individuals confirmed by this technique [ 22 ] (53).…”

Section: Discussioncontrasting

confidence: 59%

“…However, the possibility of contamination and the difficulties of interpreting positive cases as disease or colonization are the main limitations. Although several commercial kits for the detection of M. pneumoniae, C. pneumoniae , and L. pneumophila are now available [ 10 , 13 – 15 ], limited information is available in the literature regarding the validation process of such tests. The existing studies have limited information about the clinical condition of the study population in which the tests were validated, the samples used, and the molecular targets; some studies compared only the commercial kit with another in-house or commercial molecular test, without using any other accepted reference tests (culture or paired serology).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of serological methods with PCR-based methods for the diagnosis of community-acquired pneumonia caused by atypical bacteria

Herrera

Aguilar

Rueda

et al. 2016

J Negat Results BioMed

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BackgroundThe diagnosis of community-acquired pneumonia (CAP) caused by Legionella pneumophila, Mycoplasma pneumoniae, and Chlamydophila pneumoniae is traditionally based on cultures and serology, which have special requirements, are time-consuming, and offer delayed results that limit their clinical usefulness of these techniques. We sought to develop a multiplex PCR (mPCR) method to diagnosis these bacterial infections in CAP patients and to compare the diagnostic yields obtained from mPCR of nasopharyngeal aspirates (NPAs), nasopharyngeal swabs (NPSs), and induced sputum (IS) with those obtained with specifc PCR commercial kits, paired serology, and urinary antigen.ResultsA total of 225 persons were included. Of these, 10 patients showed serological evidence of L. pneumophila infection, 30 of M. pneumoniae, and 18 of C. pneumoniae; 20 individuals showed no CAP. The sensitivities were mPCR-NPS = 23.1 %, mPCR-IS = 57.1 %, Seeplex®-IS = 52.4 %, and Speed-oligo®-NPA/NPS = 11.1 %, and the specificities were mPCR-NPS = 97.1 %, mPCR-IS = 77.8 %, Seeplex®-IS = 92.6 %, and Speed-oligo®-NPA/NPS = 96.1 %. The concordance between tests was poor (kappa <0.4), except for the concordance between mPCR and the commercial kit in IS (0.67). In individuals with no evidence of CAP, positive reactions were observed in paired serology and in all PCRs.ConclusionsAll PCRs had good specificity but low sensitivity in nasopharyngeal samples. The sensitivity of mPCR and Seeplex® in IS was approximately 60 %; thus, better diagnostic techniques for these three bacteria are required.Electronic supplementary materialThe online version of this article (doi:10.1186/s12952-016-0047-y) contains supplementary material, which is available to authorized users.

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Section: Discussioncontrasting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Comparison of serological methods with PCR-based methods for the diagnosis of community-acquired pneumonia caused by atypical bacteria

Herrera

Aguilar

Rueda

et al. 2016

J Negat Results BioMed

View full text Add to dashboard Cite

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“…In another series, the prevalence of M. pneumoniae ranged from 1% to 38% 4,7,22,26,28–30 . The differences in some large series may depend on the geographic variations 4–26 . M. pneumoniae was associated with more days of fever and prescription of ambulatory beta‐lactams.…”

Section: Discussionmentioning

confidence: 87%

“…pneumoniae, several reports show a high correlation between detection by means of PCR and serology. 4,9,[18][19][20] For viruses, there are no reports showing such a correlation, and other ways to infer causality are currently unavailable for most clinical practitioners.…”

Section: Discussionmentioning

confidence: 99%

“…The use of sensitive PCR assays for testing NPA specimens revealed a high prevalence of CAP caused by respiratory viruses and M. pneumoniae . Although the accuracy of upper respiratory tract samples to infer causality has been questioned, in the case of M. pneumoniae , several reports show a high correlation between detection by means of PCR and serology 4,9,18–20 . For viruses, there are no reports showing such a correlation, and other ways to infer causality are currently unavailable for most clinical practitioners.…”

Section: Discussionmentioning

confidence: 99%

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Viruses and Mycoplasma pneumoniae are the main etiological agents of community‐acquired pneumonia in hospitalized pediatric patients in Spain

Otheo

Rodríguez

Moraleda

et al. 2021

Pediatric Pulmonology

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Mycoplasma pneumoniae in Children With and Without Community-acquired Pneumonia. What do PCR and Serology Say?

Copete

Vera

Herrera

et al. 2020

Pediatric Infectious Disease Journal

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Background: IgM titers of Mycoplasma pneumoniae can remain high for months or years, and specific DNA can be detected in asymptomatic people. Methods: We compared the performance of serology and PCR in children with and without community-acquired pneumonia (CAP) for the diagnosis of M. pneumoniae. Results: In children with CAP, a positive test by M. pneumoniae (PCR and/or paired serology or both) were found in 13.9%. Of these, 10.3% were positive by multiplex PCR (Seeplex-Seegen), and 6.7% exhibited quadrupled titers (22 for IgG, 6 for IgM and 5 for both). Both tests were positive in 2.8% of cases. In the group without CAP, 3.3% were positive by PCR. Thirty-two percent of children with CAP and 38.3% of healthy children had IgM titers >11 in the acute phase. Conclusions: The detection of IgM is not useful for diagnosing acute M. pneumoniae infection, and a positive PCR result can be due to colonization and not infection. New and better diagnostic techniques are required.

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Comparison of 2 molecular assays and a serologic test in diagnosing Mycoplasma pneumoniae infection in paediatrics patients

Cited by 6 publications

References 15 publications

Comparison of serological methods with PCR-based methods for the diagnosis of community-acquired pneumonia caused by atypical bacteria

Comparison of serological methods with PCR-based methods for the diagnosis of community-acquired pneumonia caused by atypical bacteria

Viruses and Mycoplasma pneumoniae are the main etiological agents of community‐acquired pneumonia in hospitalized pediatric patients in Spain

Mycoplasma pneumoniae in Children With and Without Community-acquired Pneumonia. What do PCR and Serology Say?

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