Comparison dose effects of vitamin C and zinc administration to inhibit klebsiella pneumoniae biofilms formation

Abstract

Background: Klebsiella pneumoniae is a Gram-negative bacterium that form biofilm and causes various infections. Biofilms have important role in resistance to antibiotics. Alternative agents that can inhibit biofilms formation with minimal side effects is required. This study show effects of vitamin C and zinc to inhibit Klebsiella pneumoniae biofilms formation. Method: This experiment used clinically stored Klebsiella pneumoniae isolates using controls. Isolates other than control are exposed with vitamin C and zinc oral preparations with various doses invitro then Optical Density and percentage reduction in biofilm is calculated and compared. Results: Vitamin C and zinc had invitro-inhibiting effect on biofilms formation of Klebsiella pneumoniae at all doses. The differences in decrease of Optical Density biofilm from the doses used after statistical testing was significant (P < 0.05). The smallest Optical Density and the largest percentage of Optical Density reduction of biofilms is found in vitamin C 1000 mg and zinc 50 mg. Conclusion: There are differences in inhibition of biofilm formation Klebsiella pneumoniae in the administration of vitamin C and zinc, with higher dose of vitamin C and zinc, the inhibition of biofilms formation is greater.