Comparison between Two-Layer Density Gradient and Three-Layer Density Gradient Technique for Sperm Preparation at Aster Fertility Clinic, Dr. Hasan Sadikin General Hospital

Astarto, Nanang Winarto; Tjahyadi, Dian; Jatnikasari, Sintya

doi:10.15850/ijihs.v2n1.286

Cited by 2 publications

(3 citation statements)

References 9 publications

(11 reference statements)

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“…The pellet is then re-suspended with gradient media again in DGC technique with and without TAD 600mg Glutathione, so spermatozoa migrate through density of media and then from pellet to upper media, the data of this study showed increase concentration rate of sperm was in DGC with TAD 600mg Glutathione technique. These results were in contrast with other studies using DGC techniques compared with other techniques (12).…”

Section: Discussioncontrasting

confidence: 99%

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Untitled

2018

IJEIR

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Background: One of the major concerns among the male reproductive health is the inability to achieve parenthood even after regular unprotected intercourse. The efficiency of the sperm selection techniques is expressed as the concentration of spermatozoa with normal motility. Discontinuous density gradient centrifugation (DGC) method widely used over the last decade due to effectiveness and reproducibility in recovering most motile sperm, which separates sperm based on their motility, size, and density differential. Glutathione act as an amino acid donor during spermatogenesis. If the levels of glutathione are too low during spermatogenesis, the number of mature and morphologically normal spermatozoa generated will be decreased. Objectives:The aim of this study was to assess the beneficial role of TAD 600mg Glutathione combined with DGC technique for activation of human sperm of asthenozoospermic, oligozoospermic and normozoospermic subjects compared with the DGC technique alone. Patients, Materials and Methods:Sixty males were involved in this study, divided into three groups, (twenty asthenozoospermic, Twenty oligozoospermic and twenty normozoospermic subjects)during their attendance to the Infertility Clinic at High Institute for Infertility Diagnosis and Assisted Reproductive Technologies; Al-Nahrain University. Semen samples were obtained, and seminal fluid analysis was assessed. Each semen sample was divided into three parts. The first part prepared as in vitro sperm characterization before activation, the second part using density gradient centrifugation technique, while the last part prepared using density gradient centrifugation combined with TAD 600mg Glutathione. Results: After in vitro sperm activation for asthenozoospermic, oligozoospermic and normozoospermic samples a significant increase was observed in the sperm function parameters including sperm concentration, motility and morphologically normal sperm percentage as compared to pre-activation using combined techniques (density gradient centrifugation and TAD 600mg Glutathione) as compared to DGC technique alone. Conclusions:TAD 600mg Glutathione combined to the DGC technique was found to give higher significant results on sperm function parameters (sperm concentration, motility and morphology) when using a low quality of semen samples such as decreased sperm motility as compared with DGC technique alone.

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Section: Discussioncontrasting

confidence: 99%

“…Density gradient centrifugation technique used for the separation of sperms has been golden technique In a lot of ART's laboratories because of its performance that are easily and quickly resulting in highly quality of sperm motility (8).…”

Section: -Discontinuous Density Gradient Centrifugation (Dgc) Technimentioning

confidence: 99%

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2018

IJEIR

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“…The importance of sperm preparation techniques in ART's, is to obtain the highest sperm recovery and in vitro treatment of spermatozoa to improve their function like, motility and improve or maintain the functional capacity for successful fertilization through supplementation of protective media (15) .…”

Section: Discussion:-mentioning

confidence: 99%

The New Beneficial Application of Caffeine as a Promoting Factor of Sperm Motility Combined With Density Gradient Centrifugation Technique for Best Yield for Asthenozoospermic and Oligozoospermic Men.

Hamza¹,

Selman²,

Mossa.³

2017

IJAR

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Background: Seminal fluid of males is varied in quality, not only between males but also within a single sample. A verity of in vitro sperm preparation techniques were developed to separate normal and motile spermatozoa from other constituents of sample to provide successful assisted reproductive techniques. Objectives:The goal of this study was to compare the effects of the four in vitro sperm activation techniques on sperm function parameters specially sperm motility ofasthenozoospermicand oligozoospermicsemen samples. Subjects, Materials and Methods:Thirty-five asthenozoospermic and fifteen oligozoospermic men were participated in this study during their attendance to the Infertility Clinic at High Institute for Infertility Diagnosis and Assisted Reproductive Technologies; Al-Nahrain University. Collection of semen samples and seminal fluid analysis was done according to WHO (1999) and WHO (2010). Each semen sample was divided into four parts. The first one prepared as in vitro sperm activation using the direct swim-up technique, the second using indirect swim-up technique,the third using density gradient centrifugation technique, while the last part prepared using density gradient centrifugation and Caffeine(combined techniques). Results: A significant increase was observed in the sperm function parameters including sperm motility and morphologically normal sperm for asthenozoospermic and oligozoospermic samples when using density gradient centrifugation and Caffeine as compared to density gradient centrifugation alone.Also, there was a significant increase was observed in the same sperm function parameters when using density gradient centrifugation as compared to direct swim-up technique and indirect swim-up technique. Conclusion: In this study, the outcome of sperm function parameters using combined density gradient technique with Caffeine were higher than other techniques, especially when using a low quality of semen samples such as decreased sperm motility, therefore it was fit in cases ofasthenozoospermia and oligozoospermia which are taken as examples of infertile group in this study.

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Comparison between Two-Layer Density Gradient and Three-Layer Density Gradient Technique for Sperm Preparation at Aster Fertility Clinic, Dr. Hasan Sadikin General Hospital

Cited by 2 publications

References 9 publications

Untitled

Untitled

The New Beneficial Application of Caffeine as a Promoting Factor of Sperm Motility Combined With Density Gradient Centrifugation Technique for Best Yield for Asthenozoospermic and Oligozoospermic Men.

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