Comparison between two kinds of cigarette smoke condensates (CSCs) of the cytogenotoxicity and protein expression in a human B-cell lymphoblastoid cell line using CCK-8 assay, comet assay and protein microarray

Cyto-genotoxic effects of smoke from commercial filter and non-filter cigarettes on human bronchial and pulmonary cells

“…Some recent studies have used the comet assay to assess the cyto-genotoxicity of cigarette-smoke condensate (CSC) from commercial cigarettes with different tar yields [41][42][43] on human peripheral lymphocytes and human B-lymphoblastoid cells (cell line HMy2.CIR) after 4 h of exposure. In particular, Lou et al [43] evaluated the cyto-genotoxicity of 12 different CSCs by use of five in vitro assays (CCK-8 and Neutral Red viability assays, cell apoptosis assay, comet assay and micronucleus assay), and found that cyto-genotoxicity of the different brands of cigarettes varied greatly and demonstrated the high sensitivity of the comet assay in assessing the genotoxicity induced by CSC.…”

“…In particular, Lou et al [43] evaluated the cyto-genotoxicity of 12 different CSCs by use of five in vitro assays (CCK-8 and Neutral Red viability assays, cell apoptosis assay, comet assay and micronucleus assay), and found that cyto-genotoxicity of the different brands of cigarettes varied greatly and demonstrated the high sensitivity of the comet assay in assessing the genotoxicity induced by CSC.…”

The p-ERK–p-c-Jun–cyclinD1 pathway is involved in proliferation of smooth muscle cells after exposure to cigarette smoke extract

“…As a control, corresponding concentrations of PBS were added to cells. Cells were then cultivated in 5% CO 2 , at 37°C in an incubator for 48 h. The medium was then removed and replaced with fresh medium containing 10% Cell Counting Kit-8 (CCK-8) reagent (Dojindo, Kumamoto, Japan) [12], and the cells cultivated in 5% CO 2 , at 37°C in an incubator for 2 h; in addition, blank wells were set up that contained medium with 10% CCK-8 only. Absorbance values were measured at 450 nm.…”

Enhanced Anti-Tumor (Anti-Proliferation) Activity of Recombinant Human Interleukin-29 (IL-29) Mutants Using Site-Directed Mutagenesis Method

“…Inhibitions of tumor cells growth acted by hIL-29 or hIL-29 mutants were determined by CCK-8 assay [13]. Absorbance value was measured with a microplate reader at a test wavelength of 450 nm against a reference wavelength of 630 nm.…”