This study developed an intelligent control system to improve the climate management under greenhouse. The proposed regulation structure relies on an adaptive neuro-fuzzy inference system (ANFIS) inverse model. At first, the controller was trained with practical database using Matlab tool. Afterwards, it was simulated through a greenhouse model. Finally, it has been validated in real time based on an automated experimental greenhouse. Simulation studies and experimental results proved that the adopted control strategy is able to yield good regulation despite of the greenhouse system complexity and the continuous changes of environmental conditions. Moreover, employing our strategy reduced settling time compared to PID and fuzzy based controllers which are commonly utilized in the control engineering field.