Comparison between transcriptomic responses to short-term stress exposures of a common Holarctic and endemic Lake Baikal amphipods

Drozdova, Polina; Rivarola-Duarte, Lorena; Bedulina, Daria; Axenov-Gribanov, Denis V.; Schreiber, S; Gurkov, Anton; Shatilina, Z. M.; Vereshchagina, Kseniya; Lubyaga, Yulia; Otto, Christian; Jühling, Frank; Busch, Wibke; Jakob, Lena; Lucassen, Magnus; Sartoris, Franz-Josef; Hackermüller, Jörg; Hoffmann, Steve; Pörtner, Hans‐Otto; Luckenbach, Till; Timofeyev, Maxim A.; Stadler, Peter F.

doi:10.1186/s12864-019-6024-3

Cited by 19 publications

(18 citation statements)

References 44 publications

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“…Thus, in comparison to Eulimnogammarus species, the transcriptome response in G. lacustris was less pronounced. Since the metabolism of G. lacustris appears to be less well adapted to this temperature than that of the Eulimnogammarus species (Axenov-Gribanov et al, 2016) the weaker transcriptome response in this species was seen as a result of a more decreased metabolism at 6 • C (Drozdova et al, 2019b). In the present study, no relation of the degree of gene responses to the species-specific adaptation to the experimental temperature became evident.…”

Section: Gene Responsescontrasting

confidence: 60%

“…In a recent study on the whole transcriptome response of amphipods to chemical stressors, which was similarly set up as this study, it was concluded that the experimental temperature of 6 • C perturbed the transcriptome response in G. lacustris (Drozdova et al, 2019b). Thus, in comparison to Eulimnogammarus species, the transcriptome response in G. lacustris was less pronounced.…”

Section: Gene Responsesmentioning

confidence: 52%

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Changes of cellular stress response relatedhsp70andabcb1transcript and Hsp70 protein levels in Siberian freshwater amphipods upon exposure to cadmium chloride in the lethal concentration range

Протопопова

Павличенко

Luckenbach

2020

PeerJ

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The induction of cellular stress response systems, heat shock protein hsp70/Hsp70 and multixenobiotic transporter abcb1, by cadmium chloride (CdCl2) was explored in amphipod species with different stress adaptation strategies from the Lake Baikal area. Based on the lethal concentrations (LC) of CdCl2, the sensitivities of the different species to CdCl2 were ranked (24 hr LC50 in mg/L CdCl2 (mean/95% confidence interval)): Gammarus lacustris (1.7/1.3–2.4) < Eulimnogammarus cyaneus (2.9/2.1–4.0) < Eulimnogammarus verrucosus (5.7/3.8–8.7) < Eulimnogammarus vittatus (18.1/12.4–26.6). Conjugated dienes, indicating lipid peroxidation, were significantly increased after 24 hr exposures to 5 mg/L CdCl2 only in the more CdCl2-sensitive species G. lacustris and E. cyaneus. Upon treatment with 0.54 to 5.8 mg/L CdCl2 for 1, 6 and 24 hrs, hsp70 transcript levels were generally more increased after the longer exposure times and in the more CdCl2-sensitive species. Relating the CdCl2 exposure concentrations to LCx values revealed that across the species the increases of hsp70 transcript levels were comparatively low (up to 2.6-fold) at CdCl2 concentrations ≤LC50. Relative hsp70 transcript levels were maximally increased in E. cyaneus by 5 mg/L CdCl2 ($\hat {=}$LC70) at 24 hrs (9.1-fold increase above the respective control). When G. lacustris was exposed to 5 mg/L CdCl2 ($\hat {=}$LC90) for 24 hrs, the increase in hsp70 was in comparison to E. cyaneus considerably less pronounced (3.0-fold increase in hsp70 levels relative to control). Upon exposure of amphipods to 5 mg/L CdCl2, increases in Hsp70 protein levels compared to untreated controls were highest in E. cyaneus at 1 and 6 hrs (5 mg/L CdCl2 $\hat {=}$ LC70) and in E. verrucosus at 24 hrs (5 mg/L CdCl2 $\hat {=}$ LC45). Thus, when the fold increases in Hsp70 protein levels in the different amphipod species were related to the respective species-specific LCx values a similar bell-shaped trend as for hsp70 transcript levels was seen across the species. Transcript levels of abcb1 in CdCl2exposed individuals of the different amphipod species varied up to 4.7-fold in relation to the respective controls. In contrast to hsp70/Hsp70, abcb1 transcripts in CdCl2 exposed individuals of the different amphipod species did not indicate similar levels of induction of abcb1 at equal LCx levels across the species. Induction of hsp70 and abcb1 genes and Hsp70 proteins by CdCl2 in the lethal concentration range shows that these cellular responses are rather insensitive to CdCl2 stress in the examined amphipod species. Furthermore, the increase of expression of these cellular defense systems at such high stress levels suggests that induction of these genes is not related to the maintenance of normal metabolism but to mitigation of the effects of severe toxic stress.

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Section: Gene Responsescontrasting

confidence: 60%

Section: Gene Responsesmentioning

confidence: 52%

Changes of cellular stress response relatedhsp70andabcb1transcript and Hsp70 protein levels in Siberian freshwater amphipods upon exposure to cadmium chloride in the lethal concentration range

Протопопова

Павличенко

Luckenbach

2020

PeerJ

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“…Another possibility could be that the crustacyanin analog proteins were restricted to hemolymph. To check for that, we compared the expression levels in 25 transcriptomic samples of E. cyaneus in control conditions published earlier (Drozdova et al, 2019). We found that the crustacyanin analogs had much higher expression levels that were at least two or three orders of magnitude higher than those of the crustacyanin-like transcripts (Fig.…”

Section: Phylogenetic Analysis Suggests That the Diversity Of Coloratmentioning

confidence: 86%

“…The blastx command from the ncbi-blast+ package (Camacho et al, 2009) v2.2.28+ was used to search for the sequences of H. gammarus crustacyanins A2 (P80007; Keen et al, 1991a) and C1 (P80029; Keen et al, 1991b) in the published transcriptome assemblies of the studied species (GEPS01 and GEPV01 from (Naumenko et al, 2017); GHHW01 from (Drozdova et al, 2019); NCBI IDs of the assemblies are shown here and elsewhere), as well as in the reassembled data. Trinity (Grabherr et al, 2011) v2.8.5 was used to reassemble the E. vittatus transcriptome from the published raw sequencing reads (Naumenko et al, 2017); SRA NCBI: SRR3467061).…”

Section: Data Analysis and Bioinformatic Methodsmentioning

confidence: 99%

“…In addition, we wanted to know if the studied species possess proteins more similar to decapod crustacyanins than the ones identified with mass spectrometry. To predict putative crustacyanin sequences, we looked for sequences similar to H. gammarus crustacyanins A and C in the published E. cyaneus assembly (GHHW01; Drozdova et al, 2019) and E. vittatus assembly (GEPV01; Naumenko et al, 2017). Then the protein sequences were predicted and re-classified against the non-redundant NCBI protein database.…”

Section: Phylogenetic Analysis Suggests That the Diversity Of Coloratmentioning

confidence: 99%

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The level of putative carotenoid-binding proteins determines the body color in two species of endemic Lake Baikal amphipods

Drozdova

Saranchina

Morgunova

et al. 2020

PeerJ

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Color is an essential clue for intra- and interspecies communication, playing a role in selection and speciation. Coloration can be based on nanostructures and pigments; carotenoids and carotenoproteins are among the most widespread pigments in animals. Over 350 species and subspecies of amphipods (Crustacea: Amphipoda) endemic to Lake Baikal exhibit an impressive variability of colors and coloration patterns, including intraspecific color morphs. However, the mechanisms forming this diversity are underexplored, as while the carotenoid composition of several transparent, green, and red species was investigated, there have been no reports on the corresponding carotenoid-binding proteins. In this work, we analyze the coloration of two brightly colored Baikal amphipods characterized by intraspecific color variability, Eulimnogammarus cyaneus and E. vittatus. We showed that the color of either species is defined by the level of putative carotenoid-binding proteins similar to the pheromone/odorant-binding protein family, as the concentration of these putative crustacyanin analogs was higher in blue or teal-colored animals than in the orange- or yellow-colored ones. At the same time, the color did not depend on the total carotenoid content, as it was similar between animals of contrasting color morphs. By exploring the diversity of these sequences within a larger phylogeny of invertebrate crustacyanins, we show that amphipods lack orthologs of the well-studied crustacyanins A and C, even though they possess some crustacyanin-like sequences. The analysis of expression levels in E. cyaneus showed that the transcripts encoding crustacyanin analogs had much higher expression than the crustacyanin-like sequences, suggesting that the former indeed contribute to the color of these brightly colored animals. The crustacyanin analogs seem to act in a similar way to the well-studied crustacyanins in body color formation, but the details of their action are still to be revealed.

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Human impact and ecosystemic health at Lake Baikal

Brown

Gerber²,

Bedulina

et al. 2021

WIREs Water

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Lake Baikal in eastern Siberia is the deepest and (by volume) largest lake on Earth. Among the most ancient lakes, it is home to thousands of endemic species. Lake Baikal is well‐known for its water purity, especially its dominant deep‐water layers, which consist of pristine, drinking‐quality water and is inhabited by unique endemics with enormous biodiversity. But some coastal sites of this giant lake (mainly localized in isolated shallow bays and littoral zones close to settlements and industrial sites) are suffering from an environmental crisis that threatens the water's quality and the ecosystem it supports. As part of the global ecosystem, Lake Baikal is already facing significant impacts from global climate change, which, in turn, increase the risks and consequences of local anthropogenic effects. Although the lake is protected by federal law, human activities are nevertheless having a growing negative impact on its ecosystem. Political, economic, and social factors result in cascading natural consequences in water quality, coastal sustainability, pollution sinks, water level management, forest maintenance, fire control and biodiversity. While Lake Baikal is a UNESCO World Heritage Site, known and valued around the world, it remains vulnerable to worsening threats that must be addressed if humanity is to preserve this unique ecosystem and its endemic species into the future. This article is categorized under: Water and Life > Stresses and Pressures on Ecosystems Water and Life > Conservation, Management, and Awareness Science of Water > Water Quality Engineering Water > Planning Water

show abstract

Comparison between transcriptomic responses to short-term stress exposures of a common Holarctic and endemic Lake Baikal amphipods

Cited by 19 publications

References 44 publications

Changes of cellular stress response relatedhsp70andabcb1transcript and Hsp70 protein levels in Siberian freshwater amphipods upon exposure to cadmium chloride in the lethal concentration range

Changes of cellular stress response relatedhsp70andabcb1transcript and Hsp70 protein levels in Siberian freshwater amphipods upon exposure to cadmium chloride in the lethal concentration range

The level of putative carotenoid-binding proteins determines the body color in two species of endemic Lake Baikal amphipods

Human impact and ecosystemic health at Lake Baikal

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