Abstract:Traditional microbiological diagnostic techniques such as baciloscopy and culture were compared with polymerase chain reaction (PCR) for isolation of Mycobacterium spp from human patient secretions. PCR was standardized to diagnose tuberculosis. Three pairs of primers were used: one for Mycobacterium spp detection, one for the Mycobacterium tuberculosis complex, and one specific for Mycobacterium bovis. Two hundred clinical samples (195 of sputum and 5 of urine) were sent to the Mycobacterium Laboratory of Ado… Show more
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.