Comparison between three molecular methods for detection of blood melanoma tyrosinase mRNA. Correlation with melanoma stages and S100B, LDH, NSE biochemical markers

Santonocito, Concetta; Concolino, Paola; Lavieri, Maria Michela; Ameglio, Franco; Gentileschi, Stefano; Capizzi, Rodolfo; Rocchetti, Sandro; Amerio, Pierluigi; Castagnola, Massimo; Zuppi, Cecilia; Capoluongo, Ettore

doi:10.1016/j.cccn.2005.05.031

Cited by 23 publications

(16 citation statements)

References 24 publications

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“…Some studies have shown that a change in CTCs can occur with treatment and could act as an indirect marker of therapy effectiveness (6,8,24). Reynolds et al at New York University have shown that treatment with a melanoma vaccine can decrease the percentage of CTC-positive patients (12).…”

Section: Discussionmentioning

confidence: 99%

Circulating Tumor Cells in Melanoma: A Review of the Literature and Description of a Novel Technique

Steen

Nemunaitis²,

Fisher³

et al. 2008

Baylor University Medical Center Proceedings

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Melanoma is a prevalent and deadly disease with limited therapeutic options. Current prognostic factors are unable to adequately guide treatment. Circulating tumor cells are a disease-specific factor that can be used as a prognostic variable to guide therapy. Most research to date has focused on identification of circulating tumor cells using various methods, including polymerase chain reaction. These techniques, however, have poor sensitivity and variable specificity and predictive significance. A recently developed technology to identify circulating tumor cells is the CellSearch system. This system uses immunomagnetic cell labeling and digital microscopy. This technology may provide an alternative method to identify circulating tumor cells in patients with advanced-stage melanoma and function as a prognostic factor. We review the literature on circulating tumor cells in melanoma and present data collected at our institution using the CellSearch system in nine patients with stage III or IV melanoma.

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Section: Discussionmentioning

confidence: 99%

Circulating Tumor Cells in Melanoma: A Review of the Literature and Description of a Novel Technique

Steen

Nemunaitis²,

Fisher³

et al. 2008

Baylor University Medical Center Proceedings

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“…Reverse transcriptase polymerase chain reaction (RT‐PCR) of RNA from circulating melanoma cells has been trialled for detection of hematogenous spread since 1991 (Santonocito et al., 2005; Smith et al., 1991). Although the sensitivity of this technique has been well demonstrated, quality control for diagnostic use remains a problem because of the potential for both false positive and false negative results (Ghossein and Bhattacharya, 2000).…”

Section: Melanoma Markersmentioning

confidence: 99%

Molecular markers of circulating melanoma cells

Medic¹,

Pearce²,

Heenan³

et al. 2006

Pigment Cell Research

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SummaryOf all skin cancers, cutaneous malignant melanoma (CMM) is the most aggressive and the life expectancy of patients with lymphatic or systemic metastases is dramatically reduced. Understandably therefore, scientists and clinicians have focused on improving diagnostic and prognostic techniques. Of these, perhaps the most promising are multimarker real-time RT-PCR and microarray for detection of circulating CMM cells in peripheral blood. While the optimal set of markers is still to be identified that can accurately assess disease severity and progression at all clinical stages of the disease, recent progress has been dramatic. Here we provide an exhaustive review of recent studies in which a variety of markers are assessed. Moreover, the efficacy of the markers relative to clinical stage is discussed in light of experimental findings. From these studies, it is apparent that researchers are now much closer to defining a set of markers of circulating cells that can be utilized in routine diagnostic tests.

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“…All patients were females who underwent vulvectomy for vulvar carcinoma or melanoma with an inguinal defect caused by lymphadenectomy. 6,16 Inguinal defect was in 20 cases a wide subcutaneous defect following extended groin dissection with 3 of them including also an inguinal skin resection because of proximity of pathologic nodes, and in three cases was caused by the removal of a big lymphocele, developed after groin dissection. 17 Age, height, weight, and body mass index (BMI) of patients were recorded.…”

Section: Methodsmentioning

confidence: 99%

Radioanatomical Study of the Pedicle of the Superficial Circumflex Iliac Perforator Flap

Gentileschi

Servillo

Bonis

et al. 2019

J reconstr Microsurg

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Background The superficial circumflex iliac perforator (SCIP) flap has many ideal features, such as fast dissection, possibility to harvest thin, pliable, wide skin island, and concealed donor site scar. In spite of these features, its use was limited because of the wide anatomical variation of the pedicle, which is relatively shorter and has a smaller caliber than other more popular perforator flaps. Several names were given to the branches and perforators in the literature, thus adding confusion to the understanding of its anatomy. Methods We performed a surgical and a radioanatomical study of the SCIP pedicles analyzing high-resolution contrast-enhanced computed tomography (CT) scan of 95 groins, with particular attention to the deep branch (DB) of the superficial circumflex iliac artery (SCIA). Twenty-three of these patients were also studied by detecting the surgical anatomy during SCIP flap harvest. We employed a system of coordinates based on the line between pubic tubercle (PT) and anterior superior iliac spine (ASIS) to describe the position of the perforator of the DB. Results We found a 100% correlation between surgical and radiological findings. The length of the DB from the origin to the point in which its perforator pierced the sartorius fascia ranged from 1.6 to 6.5 cm, mean = 3.62 ± 0.92 cm. The distance between the origin of the DB and the inguinal ligament ranged from 1.1 to 7.5 cm, mean = 2.8 ± 1 cm. The perforator of the DB could be found in 91% of the cases within a box of 4 cm × 3 cm drawn caudally to the line joining the PT with the ASIS. This vessel can show a vertical or horizontal course in the subcutaneous layer. Conclusion Our findings confirm other previous studies and add new information about the position and the course of the perforator of the DB of the SCIA. Important features of the SCIP pedicles can be investigated by the color Doppler ultrasound and CT scan.

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Comparison between three molecular methods for detection of blood melanoma tyrosinase mRNA. Correlation with melanoma stages and S100B, LDH, NSE biochemical markers

Cited by 23 publications

References 24 publications

Circulating Tumor Cells in Melanoma: A Review of the Literature and Description of a Novel Technique

Circulating Tumor Cells in Melanoma: A Review of the Literature and Description of a Novel Technique

Molecular markers of circulating melanoma cells

Radioanatomical Study of the Pedicle of the Superficial Circumflex Iliac Perforator Flap

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