Comparison between thermal hydrolysis and enzymatic proteolysis processes for the preparation of tilapia skin collagen hydrolysates

Wang, Wei; Li, Zhonglei; Liu, Junzhong; Wang, Yuejun; Liu, Shanhong; Sun, Ming

doi:10.17221/49/2012-cjfs

Cited by 23 publications

(17 citation statements)

References 14 publications

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“…The scavenging effects of TCP on DPPH free radicals were measured as described in Wang et al (2013). Briefly, 2 mL of collagen peptide samples (6~18 mg mL À1 ) was added to 2 mL of 0.1 mM DPPH in ethanol (95%).…”

Section: Dpph and Abts Free Radicals-scavenging Activitiesmentioning

confidence: 99%

Antioxidant and hypoglycaemic effects of tilapia skin collagen peptide in mice

Zhang

Chen

Jiang³

et al. 2016

Int J of Food Sci Tech

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Summary In this study, tilapia collagen peptide (TCP) was prepared by alcalase hydrolysis of tilapia skin, and the antioxidant and hypoglycaemic effects of TCP were investigated. The results showed that TCP possessed DPPH and ABTS+ free radicals‐scavenging activities. In diabetic mice in which diabetes was induced by injection of alloxan (50 mg kg−1 bw), high‐dose TCP (1.7 g kg−1 bw) and the drug metformin (1.0 g kg−1 bw) were found to reduce 31.8% and 30.3% of blood glucose levels in 25 days, respectively. Moreover, in diabetic mice receiving high‐dose TCP, antioxidant enzymes SOD and CAT were increased by 23% and 59.2%, respectively, and MDA was decreased by 39.1%. Comparing the treated high‐dose TCP group with the metformin group, there were similar SOD (61.5 U mg−1 vs. 60.2 U mg−1) and MDA (1.4 nmol mg−1 vs. 1.3 nmol mg−1), but more (~7%) CAT (359.8 U g−1 vs. 336.1 U g−1). Together, the present data, for the first time, demonstrated that TCP possessed hypoglycaemic effects in mice.

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Section: Dpph and Abts Free Radicals-scavenging Activitiesmentioning

confidence: 99%

Antioxidant and hypoglycaemic effects of tilapia skin collagen peptide in mice

Zhang

Chen

Jiang³

et al. 2016

Int J of Food Sci Tech

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“…From our study, the three kinds of CPs have fairly high protein contents reaching up to more than 85%, and the highest of them, SMG, reaches up to 95.625%. The marine hydrolysates reported previously contained total protein content at a range of 70% to 98%, at which the hydrolysate could apply as essential sources of protein (Lassoued et al, ; Pei et al, ; Wang et al, ; Xu et al, ). Furthermore, our extractive procedure is efficient, and the CPs we obtained possess high purity on peptides, as well as efficient human ingestion due to a compact molecule weight for intestine absorption, which is beneath 800 Da by MALDI‐TOF‐MS.…”

Section: Discussionmentioning

confidence: 99%

Bone‐strengthening effects and safety of compound peptides from skin of Chiloscyllium plagiosum and Mustelus griseus

Wang

et al. 2020

Food Science & Nutrition

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Fish processing produces a lot of by‐products highly containing large amount of proteins which mainly consist of collagen, implying great potential value for application as nutraceutical ingredients. In present study, two kinds of sharks, Chiloscyllium plagiosum and Mustelus griseus, were used as raw material to gain three kinds of “compound peptides” (CPs) by enzymolysis, FCP (CPs from the flesh of C. plagiosum), SCP (CPs from the skin of C. plagiosum), and SMG (CPs from the skin of M. griseus). According to a series of constituent analysis, the molecule weights of FCP, SCP, and SMG were under 800 Da; amino acids composition analysis of FCP, SCP, and SMG showed that there were high glycine, proline, and hydroxyproline and low cysteine contents in SCP and SMG, which is the characteristic of collagen peptides; their total protein contents were 87.500%, 91.875%, and 95.625%, respectively; and heavy metal contents of CPs were all beneath national standards. After three kinds of CPs were administrated intragastrically to C57BL/6 mice at a total dosage of 15 g/kg, bone‐strengthening effects of SCP and SMG were manifested by osteoblasts activity promotion, bone mineral density (BMD) increase, and marrow adipocyte number decrease, yet nonsignificant effects were shown in FCP group. No index showed toxicity of SCP and SMG in subacute toxicology trial, indicating their safety as functional foods. Herein, industrial application foundation of the skins from these two sharks was explored but more efforts should subsequently be implemented for further exploitation.

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“…Kolagen dari ikan air tawar yang telah diteliti antara lain kulit ikan nila hitam (Sahubawa dan Putra 2011), ikan mas (Motowidlo et al 2008), dan nila (Wang et al 2013). Hasil ekstraksi kolagen kulit ikan patin menggunakan metode asam dan pepsin menunjukkan bahwa kolagen kulit ikan patin memiliki stabilitas thermal yang lebih baik dibanding ikan lain (Singh et al 2011).…”

Section: Pendahuluanunclassified

Effectiveness of Alkali and Acid to Produce Collagen from Fish Skin of Striped Catifish

2017

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Cara sitasi: Devi HLNA, Suptijah P, Nurilmala M. 2017. Efektifitas alkali dan asam terhadap mutu kolagen dari kulit ikan patin. Jurnal Pengolahan Hasil Perikanan Indonesia. 20(2): 255-265. AbstrakKulit ikan merupakan salah satu sumber bahan baku alternatif yang mengandung protein kolagen dalam jumlah cukup besar untuk mengisolasi kolagen. Kolagen dalam kulit ikan umumnya diekstraksi dengan metode asam, alkali, dan enzimatis. Penelitian ini bertujuan untuk mengkaji efektifitas NaOH dan asam asetat dalam proses ekstraksi kolagen dari kulit ikan patin (Pangasius sp.). Perlakuan yang digunakan pada tahap praperlakuan adalah pemberian NaOH 0,05; 0,10; 0,15 and 0,20 M dengan waktu perendaman 2, 4, 6, 8 dan 10 jam dengan pergantian larutan NaOH yang baru setiap 2 jam. Perlakuan pada tahap ekstraksi adalah pemberian asam asetat 0,05; 0,10; 0,15 dan 0,20 M dengan waktu perendaman kulit ikan patin 1, 2, dan 3 jam. Rancangan percobaan yang digunakan untuk proses praperlakuan adalah split splot, sedangkan untuk proses hidrolisis adalah rancangan acak lengkap faktorial. Hasil penelitian menunjukkan bahwa praperlakuan dengan NaOH konsentrasi 0,05 M selama 4 jam memberikan pengaruh nyata terhadap konsentrasi protein terlarut (p<0,05). Konsentrasi asam asetat 0,15 M selama 1 jam memberikan pengaruh nyata terhadap derajat pengembangan kulit ikan pada proses hidrolisis. Rendemen yang dihasilkan sebesar 17,272%, kadar protein sebesar 86%, dan viskositas 12 cP. Hasil ekstrak kolagen dari kulit ikan patin teridentifikasi sebagai kolagen tipe I melalui analisis gugus fungsi dan elektroforesis. Kolagen dari kulit ikan patin memiliki struktur rantai α1 dan α2 dengan berat molekul rantai α yaitu 94 dan 98 kDa serta rantai β yaitu 204 kDa.Kata kunci: asam asetat, natrium hidroksida, kolagen, Pangasius sp. Effectiveness of Alkali and Acid to Produce Collagen from Fish Skin of Striped CatifishAbstract Fish skin is one of the alternative sources contained high protein to isolate collagen. Fish skin generally extracted by the method of acid, alkali and enzymes. The study aim to determine the effectiveness of NaOH and acetic acid on catfish (Pangasius sp.) skin extraction process. The concentrations of alkaline pretreatment were 0,05; 0,1; 0,15 and 0,2 M with the soaking time of 2, 4, 6, 8 and 10 h by NaOH replacement in every 2 h. The concentrations of acetic acid for hydrolisis process were 0.05; 0.1; 0.15 and 0.2 M with the soaking time of 1, 2, and 3 h. The experimental design used for pretreatment process is split splot, while for the hydrolysis process is factorial completely randomized design. The results showed that pretreatment with a concentration of 0.05 M NaOH for 4 h has a significant effect for eliminating non-collagen protein (p<0.05). The acetic acid concentration of 0.15 M for 1 h also has a significant effect on fish skin swelling. The yield of striped catfish collagen was 17.272%, the protein content was 86%, and the viscosity was 12 cP. Fish skin extract was identified as type I collagen by functional groups and electropho...

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Comparison between thermal hydrolysis and enzymatic proteolysis processes for the preparation of tilapia skin collagen hydrolysates

Cited by 23 publications

References 14 publications

Antioxidant and hypoglycaemic effects of tilapia skin collagen peptide in mice

Antioxidant and hypoglycaemic effects of tilapia skin collagen peptide in mice

Bone‐strengthening effects and safety of compound peptides from skin of Chiloscyllium plagiosum and Mustelus griseus

Effectiveness of Alkali and Acid to Produce Collagen from Fish Skin of Striped Catifish

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