Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses

Ferreira, Cíntia; Palhares, Maristela Silveira; Melo, Ubiratan Pereira de; Chiarini‐Garcia, Hélio; Maranhão, Renata de Pino Albuquerque; Gheller, Valentim Arabicano; Leme, Fabíola Oliveira Paes

doi:10.29374/2527-2179.bjvm004521

Cited by 3 publications

(3 citation statements)

References 13 publications

(21 reference statements)

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“…The animals underwent two flank laparotomies to simulate surgical stress and obtain intestinal samples used in a parallel study: one at the beginning and the other at the end of the first phase in accordance with a previously described protocol ( Ferreira et al, 2022 ). Each laparotomy lasted an average of 60 min.…”

Section: Methodsmentioning

confidence: 99%

Effects of total parenteral nutrition associated with glutamine, enteral fluid therapy with or without glutamine, and fluid therapy on the acid base and electrolyte balance of horses starved after exploratory laparotomy

Melo¹,

Palhares²,

Ferreira³

et al. 2022

Braz. J. Vet. Med.

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This study aimed to evaluate the effects of the total parenteral nutrition associated with glutamine, enteral fluid therapy with or without glutamine, and fluid therapy on the acid–base and electrolyte balance of horses starved after exploratory laparotomy. Sixteen healthy male and female adult horses of mixed breed, aged between 4 and 14 years, and having a mean body weight of 248.40 ± 2.28 kg and a body score index of 3–4 (scale of 1–5) were divided into four groups with four animals per group. After an adaptation period of 30 days, they were randomly divided into four experimental groups: enteral fluid therapy, enteral fluid therapy associated with glutamine, total parenteral nutrition associated with glutamine, and parenteral fluid therapy. The experiment was further divided into two phases: Phase 1 and Phase 2. In Phase 1, an exploratory laparotomy was performed, treatments were administered to the groups and the horses received no food or water other than those given to their respective groups. In Phase 2, the animals were re-fed. Each phase had a total duration of 144 h. Venous blood samples were collected every 24 h throughout the experimental period for blood gas and electrolyte analyses. The following parameters were evaluated: pH, partial pressure of carbon dioxide, total carbon dioxide, bicarbonate, base shift, anion gap, sodium, potassium, chloride, total calcium and magnesium. Completely randomized designs with a 4 × 7 factorial scheme (groups × harvest time) in Phase 1 and a 4 × 6 factorial scheme (groups × harvest time) in Phase 2 were used with four replications. All values were considered significant when p≤0.05 (95% probability). Blood pH, bicarbonate concentration, and base shift in the PARGL group decreased, indicating metabolic acidosis. Changes in the acid–base and electrolyte balance were more intense in the PARGL group than in the other groups. These results demonstrated the need to monitor blood gas and electrolyte balance in horses with food restriction under nutritional support or prolonged fluid therapy so that such changes are promptly corrected.

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Section: Methodsmentioning

confidence: 99%

Effects of total parenteral nutrition associated with glutamine, enteral fluid therapy with or without glutamine, and fluid therapy on the acid base and electrolyte balance of horses starved after exploratory laparotomy

Melo¹,

Palhares²,

Ferreira³

et al. 2022

Braz. J. Vet. Med.

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“…Após preparação do campo operatório foi realizada incisão vertical da pele de aproximadamente 15 centímetros de comprimento no flanco, no ponto central equidistante do rebordo costal, processos transversos das vértebras lombares e a tuberosidade coxal. A seguir, no mesmo sentido da incisão cutânea, foi realizada a incisão do músculo oblíquo abdominal externo, e em sequência, a divulsão romba dos músculos oblíquo abdominal interno e transverso abdominal e por fim, a incisão do peritônio, conforme descrito por Ferreira et al (2022). Por palpação interna, foi realizada a identificação do cólon e do intestino delgado para a realização de biópsias.…”

Section: Metodologiaunclassified

“…Biópsias da mucosa retal são de fácil coleta via colonoscopia, e o procedimento apresenta poucas complicações (Melo et al, 2014). Entretanto, biópsias intestinais (intestino delgado e/ou grosso) de espessura total fornecem análise mais detalhada e pode ser direcionada com base na aparência da serosa por meio das abordagens cirúrgicas por videolaparoscopia, celiotomia ventral e, menos comumente, laparotomia pelo flanco (Day et al, 2008;Ferreira et al, 2022). Apesar da disponibilidade de várias abordagens para a coleta de biopsias intestinais, o procedimento ainda é pouco realizado a nível hospitalar ou de campo.…”

Section: Introductionunclassified

Avaliação da eficiência da laparotomia pelo flanco para obtenção de biópsias intestinais em equinos

Melo

Palhares

Ferreira

et al. 2022

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Este estudo teve por objetivo avaliar a eficiência da laparotomia pelo flanco para obtenção de biópsias intestinais em equinos, bem como a ocorrência de complicações associadas ao procedimento. Foram utilizados 16 equinos adultos hígidos, sem raça definida, quatro machos e 12 fêmeas, com idade variando entre quatro e 14 anos e peso corporal médio de 248,40 + 2,28 kg. Todos os animais foram submetidos a duas laparotomias pelo flanco para coleta de biopsias intestinais, jejuno e cólon maior, com intervalo de 144 horas entre as coletas. Complicações incisionais foram observadas em 90% dos animais incluindo seroma/infecção da linha incisional e deiscência da linha de sutura. Apesar da ocorrência de complicações, o procedimento demonstrou-se de fácil realização e adequado para obtenção de biopsias intestinais.

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Effects of total parenteral nutrition associated with glutamine, enteral fluid therapy, with or without glutamine, and fluid therapy on the lipidogram of horses subjected to starvation after laparotomy

Melo,

Palhares,

Ferreira

et al. 2023

Arq. Bras. Med. Vet. Zootec.

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In order to evaluate the effect of total parenteral nutrition associated with glutamine and enteral fluid therapy, associated or not with glutamine, on the lipidogram of horses subjected to starvation (phase 1) and refeeding (phase 2), 16 adult healthy horses were used, mixed breed, aged between four and fourteen years and average weight of 248.40 ± 2.28kg, divided into four experimental groups, with four animals per group: Group ENTGL: enteral fluid therapy with electrolytes associated with glutamine; Group PARGL: total parenteral nutrition associated with glutamine; Group ENTFL: enteral fluid therapy with electrolytes; and Group PARFL: parenteral fluid therapy. This study was divided into two phases: phase 1 and phase 2. Phase 1 consisted of performing exploratory laparotomy and beginning of starvation, in addition to the administration of treatments, according to the group, while phase 2 consisted of re-feeding the animals. Total cholesterol values remained within the normal range for the species throughout the experimental period, but there was a slight increase during phase 1, followed by a decrease in values in phase 2. Hypertriglyceridemia in the ENFL, ENTGL and PARFL groups during phase 1 occurred because of the negative energy balance. The provision of enteral fluid therapy with electrolytes and an energy source, with or without glutamine, or parenteral fluid therapy were not effective in reversing the effects of negative energy balance. Thus, therapeutic protocols that combine enteral or parenteral fluid therapy for prolonged periods and food deprivation need additional nutritional support to avoid the deleterious effects of negative energy balance.

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Comparison between the techniques of inclusion in glycol methacrylate (GMA)- based plastic resin and paraffin for evaluation intestinal morphometry in horses

Abstract: Comparison between the techniques of inclusion in glycol methacrylate (GMA)-based plastic resin and paraffin for evaluation intestinal morphometry in horses.

Cited by 3 publications

References 13 publications

Effects of total parenteral nutrition associated with glutamine, enteral fluid therapy with or without glutamine, and fluid therapy on the acid base and electrolyte balance of horses starved after exploratory laparotomy

Effects of total parenteral nutrition associated with glutamine, enteral fluid therapy with or without glutamine, and fluid therapy on the acid base and electrolyte balance of horses starved after exploratory laparotomy

Avaliação da eficiência da laparotomia pelo flanco para obtenção de biópsias intestinais em equinos

Effects of total parenteral nutrition associated with glutamine, enteral fluid therapy, with or without glutamine, and fluid therapy on the lipidogram of horses subjected to starvation after laparotomy

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