Comparison between the Hybrid Capture II Test and an SPF1/GP6+ PCR-Based Assay for Detection of Human Papillomavirus DNA in Cervical Swab Samples

Huang, Shang Lang; Chao, Angel; Hsueh, Swei; Fang, Chao; Huang, Ching-Shui; Yang, Jung Erh; Lin, Ching Yu; Yan, Chiu Cho; Chou, Hung Hsueh; Huang, Kuo‐Lun; Huang, Hsuan-Rong; Wu, Ting-Jung; Tseng, Mao Jung; Qiu, Jian Tai; Lin, Cheng Tao; Chang, Ting Chang; Lai, Chyong‐Huey

doi:10.1128/jcm.44.5.1733-1739.2006

Cited by 67 publications

(88 citation statements)

References 33 publications

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“…12 SPF1/GP61 PCR followed by revert blotting with a HPV genechip (Easychip 1 HPV Blot, Kingcar, Taiwan) was found sensitive and reproducible for HPV DNA detection and genotyping in cervical swab specimens as well as paraffin-embedded tissues in our previous studies. [13][14][15] The HPV sensitivity and reliability is comparable to HC II for cervical swab samples. 13 Prophylactic HPV vaccines have been designated against the most common oncogenic HPV types 16 and 18.…”

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confidence: 84%

“…[13][14][15] The HPV sensitivity and reliability is comparable to HC II for cervical swab samples. 13 Prophylactic HPV vaccines have been designated against the most common oncogenic HPV types 16 and 18. 16,17 Knowledge of baseline population-based HPV genotype distribution is important as a reference to assess the effect of prophylactic HPV vaccines on HPV infection after implementation of vaccination.…”

mentioning

confidence: 84%

“…DNA was extracted according to the protocol for isolation of total DNA from cultured animal cells (Qiagen, Valencia, CA). 13,14 Finally, 100 ll of DNA solution was eluted and 1 ll of the aliquot was used for polymerase chain reaction (PCR) amplification.…”

Section: Sample Collection and Dna Extractionmentioning

confidence: 99%

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Cervical cancer screening program integrating Pap smear and HPV DNA testing: A population‐based study

Chao

Hsu

Lai

et al. 2008

Intl Journal of Cancer

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We conducted a population-based cohort study to evaluate the complementary value of HPV testing to Papanicolaou (Pap) smear and the prevalence and genotype distribution of HPV in Taiwan. In this report, we described the design of the whole study and analyzed the cross-sectional results. Female residents (age 30 years) of Taoyuan, Taiwan were invited. After signing informed consent, every participant had a Pap smear and a HPV testing. Patients with Pap atypical squamous cell of undetermined significance (Group I) or those with HPV-positive but normal cytology (Group II) were referred for a colposcopic examination. A total of 10,014 women were eligible. The overall HPV prevalence was 10.8% (95% confidence interval 10.5%-11.4%) in the study population. A total of 37 types of HPV were identified and the leading three were HPV-52, -18 and -58. There was a significant positive correlation of HPV prevalence with older age, postmenopausal status, current-user of oral contraceptives and never-user of hormone replacement therapy. Past users of oral contraceptives and never users of Pap were associated with higher risk of abnormal Pap, while age 40-49 strata had lower risk. Fifty-nine cases of cervical intraepithelial neoplasia (CIN) 2 from Group I and additional 11 from Group II were identified. The improvement of sensitivity with additional HPV testing was 15.3%. Besides, no specific subgroup was found to most benefit from the combined strategy. The value of adding HPV test to conventional Pap smear has to be evaluated after longer-term follow-up of this population-based cohort. ' 2008 Wiley-Liss, Inc.Key words: cervical cancer screening; Pap smear; human papillomavirus; polymerase chain reaction (PCR); prevalence Cervical cancer continues to be one of the leading female genital cancers worldwide. 1 The cervical cancer burden in Taiwan is still heavy with the incidence of invasive cancer being 24.5/ 100,000, which ranked the fifth place of cancer death in women. 2 Traditionally, primary screening of cancer precursors remains based on cytological classification of cervical smears. Conventional Papanicolaou (Pap) test or monolayer cytology provided sensitivity rates ranged from 30% to 87% and specificity rates from 86% to 100%. 3 Since human papillomavirus (HPV) has been established as a well-recognized etiologic agent of cervical neoplasia, HPV DNA testing is a promising alternative or complementary test to improve the efficacy of cervical screening and probably cost-effective, depending on screening interval. 4,5 Cervical cancer screening programs integrating HPV testing have been advocated in United Kingdom, United States, South Africa, Thailand and Germany. [6][7][8][9][10] Hybrid Capture assay (HCII) (Digene Corporation, Gaithersburg, MD) is the most widely used method of HPV DNA testing. HCII utilizes liquid hybridization of RNA probes against HPV DNA followed by signal amplification, has been validated for its reproducibility. 11 Methods using PCR is more sensitive and quality assays were generally consistent for...

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Cervical cancer screening program integrating Pap smear and HPV DNA testing: A population‐based study

Chao

Hsu

Lai

et al. 2008

Intl Journal of Cancer

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“…Besides, the lowest rates were noted among women with HPV-16 (49%) and HPV-31 (50%). 16 Of 1,203 US women (aged [16][17][18][19][20][21][22][23] accrued in the placebo arm of a multicenter HPV vaccine trial had 6-monthly follow-up for 4-years, the type-specific clearance rates at 3 years were 98.2, 100, 85.3 and 91.1%, for HPV-6, -11, -16 and -18, respectively. 31 In our study, HPV-52 had the lowest type-specific clearance rates (30.8%) and HPV-58 had the highest (63.4%) at 3 years.…”

Section: Discussionmentioning

confidence: 99%

“…The SPF1/GP61 consensus primers 5,20,22 were used to amplify a fragment of approximately 184 bp in the L1 open reading frame. The protocol was detailed previously.…”

Section: Spf1/gp61 Pcrmentioning

confidence: 99%

Host and viral factors in relation to clearance of human papillomavirus infection: A cohort study in Taiwan

Lai

Chao

Chang

et al. 2008

Intl Journal of Cancer

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Human papillomavirus (HPV) persistence is essential for cervical cancer development. We accrued nested-cohort subjects from a population-based study to investigate the host and viral factors related to outcome of HPV infection. Women (age ≥ 30 years old) with HPV-positive but normal cytology and negative colposcopy were invited to participate. After signing informed consent, every participant completed a structured questionnaire and had 6-monthly follow-ups of Pap smear, HPV testing and colposcopy. Total and type-specific HPV clearance rates as well as host and viral factors associated with clearance in 3-year longitudinal followup were analyzed. Adjusted hazard ratios (HRs) of progression to ≥ cervical intraepithelial neoplasia (CIN) 2 according to baseline HPV of the women with normal cytology were calculated from national registry database. Among the 626 eligible women, 526 (median age 47, 29-75) were enrolled and 412 returned for followup at least once. The median follow-up of enrolled subjects was 23 months (range 6.8-39). The 3-year cumulative total HPV clearance rate was 49.0% (95% confidence interval [CI]: 43.3-54.7%). The median 3-year cumulative type-specific HPV clearance rate was 50.0% (range 0-100.0%) with a median time to clearance of 12.4 months (6.4-24.5). Older age was associated with significantly decreased total HPV clearance and decreased type-specific clearance in HPV-18 and -53, while high viral load was associated with decreased total and type-specific clearance. After adjusting confounding variables, the HR of developing CIN2 in baseline HPVpositive women was 34.0-fold (95% CI: 15.5-74.7) as compared to HPV-negative women. ' 2008 Wiley-Liss, Inc.Key words: human papillomavirus; genotype; viral load; clearance Cervical cancer is one of the leading cancers in female worldwide. 1 There is strong epidemiological and molecular biological evidence indicating that human papillomavirus (HPV) plays a central role in the etiology of cervical cancer. 2,3 Over 90% of cervical cancers and their precursor lesions are attributable to HPV infection. 4,5 However, the prevalence of genital-tract HPV infections has been reported to range from 1.4 to 25.6% in cytologically normal population. 6 It is widely believed that persistent HPV infection is necessary for the development of cervical cancer. 3,7,8 Incorporation of HPV testing into cervical cancer screening has been advocated, although specificity is decreased with expected increase in sensitivity in early detection of high-grade cervical neoplasms. 9-11 However, identifying infection of high-risk HPV causes anxiety in the individual woman. Because of lack of adequate knowledge in predicting outcome, the appropriate management is unclear except periodical follow-up.Several 8,13 HPV variants 17 have been reported associated with HPV persistence/decreased clearance, whereas ever users of oral contraceptives were associated with faster clearance. 15 Multiple infections, viral load 15 and smoking 8 were unrelated to clearance in other longitudinal follow-u...

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Human inflammatory synovial fibroblasts induce enhanced myeloid cell recruitment and angiogenesis through a hypoxia‐inducible transcription factor 1α/vascular endothelial growth factor–mediated pathway in immunodeficient mice

Rey¹,

Izquierdo²,

Caja³

et al. 2009

Arthritis & Rheumatism

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Objective. Hyperplasia and phenotypic changes in fibroblasts are often observed in chronic inflammatory lesions, and yet the autonomous pathogenic contribution of these changes is uncertain. The purpose of this study was to analyze the intrinsic ability of fibroblasts from chronically inflamed synovial tissue to drive cell recruitment and angiogenesis.Methods. Fibroblasts from patients with rheumatoid arthritis (RA) or osteoarthritis (OA), as well as fibroblasts from healthy synovial tissue and healthy skin, were cultured and subcutaneously engrafted into immunodeficient mice. Cell infiltration and angiogenesis were analyzed in the grafts by immunohistochemical studies. The role of vascular endothelial growth factor (VEGF), CXCL12, and hypoxia-inducible transcription factor 1␣ (HIF-1␣) in these processes was investigated using specific antagonists or small interfering RNA (siRNA)-mediated down-regulation of HIF-1␣ in fibroblasts.Results. Inflammatory (OA and RA) synovial fibroblasts, compared with healthy dermal or synovial tissue fibroblasts, induced a significant enhancement in myeloid cell infiltration and angiogenesis in immunodeficient mice. These activities were associated with increased constitutive and hypoxia-induced expression of VEGF, but not CXCL12, in inflammatory fibroblasts compared with healthy fibroblasts. VEGF and CXCL12 antagonists significantly reduced myeloid cell infiltration and angiogenesis. Furthermore, targeting of HIF-1␣ expression by siRNA or of HIF-1␣ transcriptional activity by the small molecule chetomin in RA fibroblasts significantly reduced both responses.Conclusion. These results demonstrate that chronic synovial inflammation is associated with stable fibroblast changes that, under hypoxic conditions, are sufficient to induce inflammatory cell recruitment and angiogenesis, both of which are processes relevant to the perpetuation of chronic inflammation.Fibroblasts are ubiquitous mesenchymal cells with vital functions during development and adulthood. They synthesize the extracellular matrix components of connective tissues needed for homeostasis and reparative responses. During development, interactions between mesenchymal and other cell lineages are necessary for the formation of many organs, and fibroblasts are sufficient to provide the positional cues required for the induction and development of the different tissues (1,2). In the adult, multiple evidence points to specialized fibroblasts as a major force in the regulation of cell homing, migration, and differentiation of highly dynamic cell populations, such as cells of the immune system or the bone marrow (3,4). With regard to the pathologic

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Comparison between the Hybrid Capture II Test and an SPF1/GP6+ PCR-Based Assay for Detection of Human Papillomavirus DNA in Cervical Swab Samples

Cited by 67 publications

References 33 publications

Cervical cancer screening program integrating Pap smear and HPV DNA testing: A population‐based study

Cervical cancer screening program integrating Pap smear and HPV DNA testing: A population‐based study

Host and viral factors in relation to clearance of human papillomavirus infection: A cohort study in Taiwan

Human inflammatory synovial fibroblasts induce enhanced myeloid cell recruitment and angiogenesis through a hypoxia‐inducible transcription factor 1α/vascular endothelial growth factor–mediated pathway in immunodeficient mice

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