Comparison between the conventional and modern techniques used for identification of Mycobacterium tuberculosis complex

Moussa, Ibrahim; Mohamed, Kh. F.; Mohamed, Marwah; Nasr, Eman; Shibl, Atef M.; Salem‐Bekhit, Mounir M.; Hatem, M. E.

doi:10.5897/ajmr11.425

Cited by 5 publications

(5 citation statements)

References 15 publications

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“…On the basis of the CIDT test the individual animal level prevalence of bovine TB was 1.67% (95% CI: 0.7-2.8). These results agreed with that reported by other researchers in Egypt 2.4%, 1.6%, and 1.8% (22)(23)(24). In contrast, several authors have reported either low prevalence rates 0.9%, 0.13%, 0.96%, and 0.30% (25)(26)(27)(28) or high values 11.6%, 11.3%, and 4.3% (5,7,29).…”

Section: Discussionsupporting

confidence: 92%

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Prevalence and risk factors of bovine tuberculosis in dairy cattle farms in Egypt

Hamed¹,

Nasr²,

Azooz³

et al. 2021

IJVS

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A cross-sectional study was carried out from November, 2015 to April, 2018 to determine the prevalence and identify the risk factors of BTB using the comparative intradermal tuberculin (CIDT) test on 5327 dairy cattle in 16 dairy herds in mid-delta, Alexandria Road and Upper Egypt districts. Questionnaires used to collect data on herd size and management. The herd and individual animal prevalence at cut-off ≥4mm were 68.75% (95%, CI: 46 to 91.4) and1.67% (95%, CI: 1.3to 2.1) respectively. The individual prevalence was significantly associated with age, breed and different location of farms and density of cattle in yard. On herd level, history of BTB in the farm, management practices, raising of different species in the same facility and newly purchased cattle are important risk factors for BTB. The postmortem examination of positive reactors revealed 66 (85.71%) out of 77slaughtered cattle with visible lesions and 11(14.29%) with non-visible lesions. Bacteriological examination revealed 74.24% (49/66) from visible lesions and 9.09% (1/11) from non-visible lesions were Mycobacterium bovis. The indirect enzyme linked immunosorbent assay (IELISA) results revealed 31(40.26%) showed positive result including 29 (93.6%) visible lesions and 2(6.4%) nonvisible lesions. In conclusion, the prevalence high in herds with poor management condition, raised different species in the same facility, had previous history of BTB infection and purchased new animals. At animal level Holstein breed, 3-6 years age, kept in high density and located in mid-delta district were at higher risk to be reactor to comparative intradermal tuberculin (CIDT) test.

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Section: Discussionsupporting

confidence: 92%

“…The isolation rate of M. bovis for non-visible reactors was 1 (9.09%). The total recovery rate of M. bovis for both visible and nonvisible reactors was 50 (64.93%), these results agreed with (22,24,53,54). and the number of atypical mycobacteria isolates was 3 (3.9%) which agree with (26,54).…”

Section: Discussionsupporting

confidence: 73%

Prevalence and risk factors of bovine tuberculosis in dairy cattle farms in Egypt

Hamed¹,

Nasr²,

Azooz³

et al. 2021

IJVS

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“…Our findings revealed a prevalence of bovine tuberculosis of 2.79% in the imported beef cattle that were under investigation. This finding nearly agrees with previous studies [ 38 , 39 ], but differs from others with a higher prevalence (11.6–24%) [ 40 – 42 ]. The low prevalence observed in our study may be attributable to the low number of tissues inspected at the slaughterhouse, or depend on other diagnostic techniques, variation in the number of examined animals [ 42 ] as well as the lack of detection of small lesions in a routine inspection.…”

Section: Discussionsupporting

confidence: 92%

Monitoring the immune response of macrophages in tuberculous granuloma through the expression of CD68, iNOS and HLA-DR in naturally infected beef cattle

Hamed,

Gómez-Laguna,

Larenas-Muñoz

et al. 2023

BMC Vet Res

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Bovine tuberculosis still represents a universal threat that creates a wider range of public and animal health impacts. One of the most important steps in the pathogenesis of this disease and granuloma formation is the phagocytosis of tuberculous bacilli by macrophages. Mycobacteria replicate in macrophages, which are crucial to the pathophysiology of mycobacterial infections; however, scarce information is available about the dynamics of the granuloma-stage immunological response. Therefore, immunohistochemistry was used in this work to evaluate the expression of CD68, iNOS, and HLA-DR in different stages of TB granulomas from naturally infected cattle with tuberculosis. Two thousand, one hundred and fifty slaughtered beef cattle were examined during the period from September 2020 to March 2022. Sixty of them showed gross tuberculous pulmonary lesions and samples were collected from all of them for histopathological examination, Ziehl-Neelsen (ZN) staining, and bacteriological culturing. Selected samples that yielded a positive result for ZN and mycobacterial culturing were subjected to an immunohistochemical study of CD68, iNOS, and HLA-DR expression by macrophages according to granuloma stages. Immunohistochemical analysis revealed that the immunolabeling of CD68+, iNOS+, and HLA-DR+ macrophages significantly reduced as the stage of granuloma increased from stage I to stage IV (P < 0.003, P < 0.002, and P < 0.002, respectively). The distribution of immunolabeled macrophages was similar for the three markers, with immunolabeled macrophages distributed throughout early-stage granulomas (I, II), and surrounding the necrotic core in late-stage granulomas (III, IV). Our results suggest a polarization to the pro-inflammatory environment and increased expression of CD68+, iNOS+, and HLA-DR+ macrophages in the early stages of granulomas (I, II), which may play a protective role in the immune response of naturally infected beef cattle with tuberculosis.

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“…The present study was conducted on imported beef cattle (mainly Zebu cattle) where 2150 slaughtered imported beef cattle aged 2 to 3 years old were examined, from which 60 cases showed gross lesions with a prevalence rate (of 2.78%, 60/2150). This nearly agrees with (Moussa et al, 2011), 2.46%; and(E. A. Nasr et al, 2016), 2.6%, but differs from(El-Sabban, Lotfy, Hammam, Dimitri, & Gergis, 1995), 24%; (Nawal et al, 2009), 23.91%;and (Ameni & Erkihun, 2007), 11.6%.…”

Section: Discussionsupporting

confidence: 75%

Prevalence and Characterization of Tuberculous Lesions in Imported Beef Cattle Slaughtered at Abu-Simbel City, Aswan Governorate

Hamed,

Abdel-samee,

Ali

et al. 2023

مجلة سوهاج لشباب الباحثين

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The number of infected cattle with tuberculosis in Egypt is increasing annually, probably as a result of the importation of live animals from countries where bovine tuberculosis is predominant. Therefore, the current study was conducted to investigate the prevalence of tuberculosis in imported beef cattle. During the period from September 2020 to March 2022, 2150 slaughtered beef cattle were examined. Sixty animals (2.78%) of them showed tuberculous lesions grossly. Depending on the gross morphologic lesions, tuberculous cases were classified into generalized tuberculosis (9 cases, 15%) and localized tuberculosis (51, 85%). Localized cases were subdivided into pulmonary tuberculosis (28, 46.66%) and extra-pulmonary tuberculosis (23, 38.33%). Specimens were collected for histopathological examination, granuloma staging, and Ziehl-Neelsen (ZN) staining. Based on the histopathological examination, the granulomas were classified into: Stage I (158, 21.43%), Stage II (187, 25.37%), Stage III (156, 21.16%), and Stage IV (236, 32.02%). In conclusion, our study reveals that imported beef cattle slaughtered in Aswan Governorate have a high incidence of tuberculosis. Accordingly, we recommend strict quarantine measures for imported beef cattle to decrease the possibility of disease transmission.

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Comparison between the conventional and modern techniques used for identification of Mycobacterium tuberculosis complex

Cited by 5 publications

References 15 publications

Prevalence and risk factors of bovine tuberculosis in dairy cattle farms in Egypt

Prevalence and risk factors of bovine tuberculosis in dairy cattle farms in Egypt

Monitoring the immune response of macrophages in tuberculous granuloma through the expression of CD68, iNOS and HLA-DR in naturally infected beef cattle

Prevalence and Characterization of Tuberculous Lesions in Imported Beef Cattle Slaughtered at Abu-Simbel City, Aswan Governorate

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