Comparison between the biofilm initiation ofCampylobacter jejuniandCampylobacter colistrains to an inert surface using BioFilm Ring Test^®

Abstract

Aims: The adhesion to an inert surface (the first step of biofilm formation) of the two main pathogenic Campylobacter species, Campylobacter jejuni and Campylobacter coli, isolated from diverse origins, was compared. Methods and Results: Adhesion assays were conducted in 96‐well, polystyrene microtiter plates using the BioFilm Ring Test® method. This new technique, based on magnetic bead entrapment, was shown to be suitable for analysing the adhesion of Campylobacter sp. strains by comparing the adhesion of four C. jejuni strains as revealed by the BioFilm Ring Test® and immunodetection. Among the 46 strains tested, C. jejuni and C. coli displayed different adhesion capabilities ranging from no adhesion to strong adhesion. However, no strain of C. coli was strongly adherent, and statistically, C. coli adhered less to an inert surface than C. jejuni. In addition, strains isolated from animals or carcasses were less adherent than those isolated from food‐processing and clinical cases. Conclusions: These observations suggest that the food environment and the human body could have selected strains with greater adhesion. Significance and Impact of the Study: The adhesion capability of strains could partly explain the cross‐contamination or re‐contamination of food products by Campylobacter. This property could provide a mode of survival for Campylobacter in the food chain.

A comparison of fluctuations of Campylobacter and Escherichia coli concentrations on broiler chicken carcasses during processing in two slaughterhouses

“…The opposite impact of defeathering on Campylobacter and E. coli can be related to potential differences in their ability to attach to the skin. Differences within even one bacterial species in the attachment to inert surfaces were previously reported (Hue et al, 2011;Sulaeman et al, 2010). Good correlation of Campylobacter and E. coli during processing was reported (Duffy et al, 2014); however, the defeathering step was not investigated.…”

Increased Biofilm Formation by Nontypeable Haemophilus influenzae Isolates from Patients with Invasive Disease or Otitis Media versus Strains Recovered from Cases of Respiratory Infections

“…Furthermore, it has been shown to be suitable for the study of adhesion with Campylobacter spp. (38).…”

Does Campylobacter jejuni Form Biofilms in Food-Related Environments?

“…Direct imaging of C. jejuni biofilms using confocal laser scanning microscopy (CLSM) or scanning electron microscopy (SEM) have been used in some studies (4,19,26,32,39). These studies have demonstrated that C. jejuni biofilms may occur in three different forms in liquid culture: (i) cells attached to inert surfaces; (ii) flocs (aggregates of cells floating in the liquid); and (iii) pellicles (aggregates of cells formed at the air-liquid interface) (4,19,40). Joshua et al (4) established that Campylobacter bacteria in the three forms mentioned above were connected via extracellular matrix using SEM.…”