Comparison between tail and jugular venipuncture techniques for blood sample collection in common chameleons (Chamaeleo chamaeleon)

Cuadrado, Mariano; Molina-Prescott, Isabel; Flores, L. Díaz

doi:10.1016/s1090-0233(02)00253-8

Cited by 19 publications

(12 citation statements)

References 8 publications

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“…The probability of lymph contamination varies with the site of puncture. A study done on chameleons showed that venipuncture from a tail vein is relatively safe from lymph contamination (Cuadrado et al 2003). Additional checking may be done after the blood analysis, by comparing variances of some blood biochemical parameters that are affected by lymph dilution, like total protein and uric acid (Cuadrado et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Biochemical and hematological profiles of a wild population of the nose-horned viper Vipera ammodytes (Serpentes: Viperidae) during autumn, with a morphological assessment of blood cells

et al. 2013

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Background: Hematological and biochemical analyses of blood can be of great importance for studying the biology of species and determining the health status of animals in both the wild and captivity. In order to determine baseline ranges for the nose-horned viper Vipera ammodytes, we determined the blood cell morphology and measured 20 hematological and 5 biochemical variables from the blood samples taken from 31 (17 males and 14 females) healthy specimens of nose-horned viper caught in the wild at the beginning of autumn. Results: Most of the measured parameters and blood cell morphology were similar to those reported for other reptile species. The males showed a significantly higher eosinophil count and higher mean cell hemoglobin (MCH) and MCH concentration values, while the females had a significantly higher mature erythrocyte count. The erythrocyte differential counts showed balanced hematopoiesis in this part of the annual cycle. Conclusions: We suggest that these results can be used as a baseline for determining hematological values and blood cell morphology for V. ammodytes, as well as in further studies including monitoring and determination of the health status of this species, especially of animals kept in captivity for antiserum production. This is the most complete report regarding hematological parameters of this endangered snake species.

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Section: Discussionmentioning

confidence: 99%

Biochemical and hematological profiles of a wild population of the nose-horned viper Vipera ammodytes (Serpentes: Viperidae) during autumn, with a morphological assessment of blood cells

et al. 2013

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“…Por outro lado, Cuadrado et al (2003) realizaram comparação entre diferentes sítios de coleta em camaleão (Chamaeleo chamaeleon) e não encontraram diferenças significativas entre amostras obtidas por punção na veia coccígea ventral e veia jugular.…”

Section: Resultsunclassified

“…Estudos foram realizados por López-Olvera et al (2003) em Testudo marginata, por Cuadrado et al (2003) em Chamaeleo chamaeleon e por Gottdenker & Jacobson (1995) em Gopherus agassizii, entretanto, até o presente, os efeitos dos diferentes sítios de coleta de sangue nos parâmetros hematológicos de tigre-d`água-americano não foram estabelecidos.…”

Section: Introductionunclassified

Efeito do sítio de venopunção nos parâmetros hematológicos em tigre-d'água-americano, Trachemys scripta elegans

Medeiros¹,

Locatelli-Dittrich

Schmidt

et al. 2012

Pesq. Vet. Bras.

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Pesq. Vet. Bras. 32(Supl. The aim of the present study was to compare two different blood collection sites of 24 red-eared sliders (Trachemys scripta elegans) from a commercial breeder, situated in Antonina, Paraná, Brasil. The animals were physically restrained and paired blood samples were collected from the dorsal coccygeal vein and the occipital sinus. The samples were collected in syringes containing heparin. After collection the samples were identified and refrigerated to posterior hematological analysis. Red blood cell and white blood count were performed using a hemocytometer. The packed cell volume and hemoglobin were determined by the microhematocrit method and cyanmethemoglobin method, respectively. Total plasma protein was determined by refractometry and differential leukocyte count was performed by the Shilling technique. There was a significant difference in the total white blood cell count and on the total plasma protein value, and on both cases the results found on the samples from the dorsal coccygeal vein were lower. The difference found on the white blood cell count was probably due to the lymph contamination of the samples, which also justifies the lower values of total plasma protein. In conclusion, the occipital sinus is more recommended blood collection site than the dorsal coccygeal vein.INDEX TERMS: Trachemys scripta, red-eared slider, blood collection, dorsal coccygeal vein.

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“…Whilst it is possible to sample blood from the caudal vein [6], and this may reduce the risk of serious injury to the snake, the volume of sample that can be collected from this vein may be insufficient for performing a suite of haematological analyses [18]. Jugular venipuncture provides an alternative option for non-lethal sample collection [37]. However, although available evidence indicates little difference in the blood chemistry of the different venipuncture sites [37], jugular puncture does not appear to have been used in studies of contaminant uptake to date.…”

Section: Bloodmentioning

confidence: 99%

“…Jugular venipuncture provides an alternative option for non-lethal sample collection [37]. However, although available evidence indicates little difference in the blood chemistry of the different venipuncture sites [37], jugular puncture does not appear to have been used in studies of contaminant uptake to date.…”

Section: Bloodmentioning

confidence: 99%

Addressing current knowledge gaps on radionuclide transfer to reptiles

et al. 2011

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Abstract. Model intercomparison exercises have identified radionuclide transfer predictions as the greatest source of uncertainty in biota dose assessments. One wildlife group for which few transfer data exist is reptiles, Given that reptiles are an important, and often protected, component of many ecosystems and that assessments of radiation impact on ecosystems are becoming increasingly necessary due to the current nuclear renaissance, there is a need to further develop our current database on transfer to reptiles or find alternative approaches to estimate reptile transfer parameters. Three approaches that have the potential to increase the availability of parameters to predict radionuclide transfer to reptiles are the use of nonlethal sampling techniques, phylogenetic relationships and allometric relationships. Non-lethal sampling is an attractive long term option for deriving transfer parameters, but the derivation of phylogenetic and allometric relationships could provide ways of predicting transfer in the interim.

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Comparison between tail and jugular venipuncture techniques for blood sample collection in common chameleons (Chamaeleo chamaeleon)

Cited by 19 publications

References 8 publications

Biochemical and hematological profiles of a wild population of the nose-horned viper Vipera ammodytes (Serpentes: Viperidae) during autumn, with a morphological assessment of blood cells

Biochemical and hematological profiles of a wild population of the nose-horned viper Vipera ammodytes (Serpentes: Viperidae) during autumn, with a morphological assessment of blood cells

Efeito do sítio de venopunção nos parâmetros hematológicos em tigre-d'água-americano, Trachemys scripta elegans

Addressing current knowledge gaps on radionuclide transfer to reptiles

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