Comparison between Stromal Vascular Fraction and Adipose Mesenchymal Stem Cells in Remodeling Hypertrophic Scars

Domergue, Sophie; Bony, Claire; Maumus, Marie; Toupet, Karine; Frouin, Éric; Rigau, Valérie; Vozenin, Marie-Catherine; Magalon, G.; Jørgensen, Christian; Noël, Danièle

doi:10.1371/journal.pone.0156161

Cited by 62 publications

(63 citation statements)

References 29 publications

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“…Our results provide evidence that SVF reduces TA fibrosis in a rat model that is generally accepted to mimic the acute phase of PD. SVF is a heterogeneous fraction containing mature endothelial cells, vascular smooth muscle cells, macrophages, regulatory T cells, fibroblasts and a large population of stem/progenitor cells [13][14][15][16][17][18]. Several preclinical and clinical studies have explored the efficacy of adipose SVF in several types of fibrotic disease, and also in animal models of erectile dysfunction and incontinence, showing positive results and a similar efficacy to ADSC treatment [13][14][15][16][17][18].…”

Section: Discussionmentioning

confidence: 99%

Intratunical injection of stromal vascular fraction prevents fibrosis in a rat model of Peyronie's disease

et al. 2019

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Objective To investigate whether local injection of autologous adipose stromal vascular fraction (SVF) can prevent the development of fibrosis and elastosis in the tunica albuginea (TA) using a rat model of the acute phase of Peyronie's disease (PD). Methods A total of 24 male 12‐week‐old Sprague–Dawley rats were divided into three equal groups: sham; PD without treatment (transforming growth factor‐β [TGF ‐β]); and PD treated with SVF 1 day after disease induction. Sham rats received two injections of vehicle into the TA 1 day apart. TGF ‐β rats received TGF‐ β1 injection and injection of vehicle 1 day later. SVF rats received TGF‐β1 injection, followed by SVF 1 day later. One month after treatment, all rats underwent measurement of intracavernosal pressure and mean arterial pressure during electrostimulation of the cavernous nerve. The rats were then killed and penises were harvested for histology and Western blot analysis. Results Erectile function was moderately reduced in the TGF‐β group and was significantly improved after SVF treatment (P < 0.05). PD rats developed areas of fibrosis with a significant upregulation of collagen III, collagen I and elastin protein expression. These fibrotic changes were prevented when treated with SVF. Conclusions Local injection of SVF may represent treatment for the acute phase of PD.

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Section: Discussionmentioning

confidence: 99%

Intratunical injection of stromal vascular fraction prevents fibrosis in a rat model of Peyronie's disease

et al. 2019

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“…was reverse transcribed using 100 units of the M-MLV reverse transcriptase (ThermoFisher Scientific, Illkirch) and PCR reactions were performed as described 16 . All details for primer sequences (SYBR Green technologies) are described in Table 1.…”

Section: Rna Extraction and Rt-qpcrmentioning

confidence: 99%

TGFβi is involved in the chondrogenic differentiation of mesenchymal stem cells and is dysregulated in osteoarthritis

Ruiz

Maumus

Fonteneau

et al. 2019

Osteoarthritis and Cartilage

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Objective: Transforming growth factor-b (TGFb) is a major regulator of cartilage homeostasis and its deregulation has been associated with osteoarthritis (OA). Deregulation of the TGFb pathway in mesenchymal stem cells (MSCs) has been proposed to be at the onset of OA. Using a secretome analysis, we identified a member of the TGFb family, TGFb-induced protein (TGFbi or bIGH3), expressed in MSCs and we investigated its function and regulation during OA. Design: Cartilage, bone, synovium, infrapatellar fat pad and bone marrow-MSCs were isolated from patients with OA or healthy subjects. Chondrogenesis of BM-MSCs was induced by TGFb3 in micropellet culture. Expression of TGFbi was quantified by RT-qPCR, ELISA or immunohistochemistry. Role of TGFbi was investigated in gain and loss of function experiments in BM-MSCs and chondrocytes. Results: TGFbi was up-regulated in early stages of chondrogenesis and its knock-down in BM-MSCs resulted in the down-regulation of mature and hypertrophic chondrocyte markers. It likely occurred through the modulation of adhesion molecules including integrin (ITG)b1, ITGb5 and N-cadherin. We also showed that TGFbi was upregulated in vitro in a model of OA chondrocytes, and its silencing enhanced the hypertrophic marker type X collagen. In addition, TGFbi was up-regulated in bone and cartilage from OA patients while its expression was reduced in BM-MSCs. Similar findings were observed in a murine model of OA. Conclusions: Our results revealed a dual role of TGFbi during chondrogenesis and pointed its deregulation in OA joint tissues. Modulating TGFbi in BM-MSCs might be of interest in cartilage regenerative medicine.

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“…The stromal fraction of WAT is made up of immune system, stem, blood, endothelial, true stromal and other types of cells, with relevant functions in the maintenance of adipocyte energy homoeostasis (Sadie van Gijsen et al, 2012), defense (Hill, Bolus & Hasty, 2014), regeneration (Domergue et al, 2016), differentiation (Gimble et al, 2011; Mitterberger et al, 2014) and others (Sumi et al, 2007; Takahara et al, 2014). Many of these functions become critical under conditions of inflammation (Lee, 2013), changing the cell composition and overall WAT metabolism (Lolmède et al, 2011; Cignarelli et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of rat adipose tissue cell recovery, and non-fat cell volume, in primary cell cultures

Rotondo

Romero

Ho-Palma

et al. 2016

PeerJ

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BackgroundWhite adipose tissue (WAT) is a complex, diffuse, multifunctional organ which contains adipocytes, and a large proportion of fat, but also other cell types, active in defense, regeneration and signalling functions. Studies with adipocytes often require their isolation from WAT by breaking up the matrix of collagen fibres; however, it is unclear to what extent adipocyte number in primary cultures correlates with their number in intact WAT, since recovery and viability are often unknown.Experimental DesignEpididymal WAT of four young adult rats was used to isolate adipocytes with collagenase. Careful recording of lipid content of tissue, and all fraction volumes and weights, allowed us to trace the amount of initial WAT fat remaining in the cell preparation. Functionality was estimated by incubation with glucose and measurement of glucose uptake and lactate, glycerol and NEFA excretion rates up to 48 h. Non-adipocyte cells were also recovered and their sizes (and those of adipocytes) were measured. The presence of non-nucleated cells (erythrocytes) was also estimated.ResultsCell numbers and sizes were correlated from all fractions to intact WAT. Tracing the lipid content, the recovery of adipocytes in the final, metabolically active, preparation was in the range of 70–75%. Cells showed even higher metabolic activity in the second than in the first day of incubation. Adipocytes were 7%, erythrocytes 66% and other stromal (nucleated cells) 27% of total WAT cells. However, their overall volumes were 90%, 0.05%, and 0.2% of WAT. Non-fat volume of adipocytes was 1.3% of WAT.ConclusionsThe methodology presented here allows for a direct quantitative reference to the original tissue of studies using isolated cells. We have also found that the “live cell mass” of adipose tissue is very small: about 13 µL/g for adipocytes and 2 µL/g stromal, plus about 1 µL/g blood (the rats were killed by exsanguination). These data translate (with respect to the actual “live cytoplasm” size) into an extremely high metabolic activity, which make WAT an even more significant agent in the control of energy metabolism.

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Comparison between Stromal Vascular Fraction and Adipose Mesenchymal Stem Cells in Remodeling Hypertrophic Scars

Cited by 62 publications

References 29 publications

Intratunical injection of stromal vascular fraction prevents fibrosis in a rat model of Peyronie's disease

Intratunical injection of stromal vascular fraction prevents fibrosis in a rat model of Peyronie's disease

TGFβi is involved in the chondrogenic differentiation of mesenchymal stem cells and is dysregulated in osteoarthritis

Quantitative analysis of rat adipose tissue cell recovery, and non-fat cell volume, in primary cell cultures

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