Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul city

Arslan, Serhat; Hussain, Kh. J.; Asmaeel, S. A.; Hassan, S. D.

doi:10.33899/ijvs.2010.5608

Cited by 3 publications

(4 citation statements)

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“…In sheep the result of indirect ELISA test(i-ELISA) total seroprevalence was (66.6% ) , in line with (17) 23.6% -100% was recorded in Mosul and (18) in Basrha (68.8%), but higher than (19) (29.34 %) , the result of milk i-ELISA test in sheep ( same flock ) were 52.83% , in reverse with (20) in Iraq. This variation in results may be due to differences in geographical region , age of animals and previous history of vaccination against Brucella in sheep and goats , subsequently cause increase titration of antibodies in serum , serological tests, can distinguish between antibodies that arise from infection and and those from vaccination, respectively, have not been developed accordance with (21).…”

Section: Resultssupporting

confidence: 69%

The prevalence of brucellosis of farm animals using serum- and milk- ELISA test in Al- Najaf province

A.Hatem

2017

Kufa Jour. Bio.

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A total of 412 milk and sera samples were collected from the same animals of 88 buffaloes, 212 cattle, 84 sheep and 28 goat were screened by indirect ELISA (i ELISA) milk confirmed with serum i- ELISA. The results of seropositive i-ELISA were 121(29.36%) from all farm animals and distributed to 20 (22.27%) buffaloes, 36 (16.98%) cattle , 56(66.6%) sheep and 9 (32.14%) goat . The positive results of milk i ELISA were 89 (21.6%) from all farm animals and distributed to 8 (9.09%) , 32 (15.09%) , 44(52.83%) and 5(17.85%) represent buffaloes , cattle , sheep and goat respectively . epidemiological informations of animals collected by questionary about ( abortion , stillbirth and previous history vaccination against brucellosis) . The prevalence was relatively higher in sheep and goat followed by buffaloes and cattle . The sera and milk i ELISA test is only the best serological tests and has a high efficiency and accuracy in the diagnosis and inexpensive and can be used to control of the disease.

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Section: Resultssupporting

confidence: 69%

The prevalence of brucellosis of farm animals using serum- and milk- ELISA test in Al- Najaf province

A.Hatem

2017

Kufa Jour. Bio.

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“…When a study used more than one screening test for the diagnosis (e.g. RBT and enzyme-linked immunosorbent assay (ELISA) technique), the more accurate test estimation, e.g., ELISA [5,20], was considered in the meta-analysis. A total of 25 studies published between 1979 and 2019 was used in the analysis.…”

Section: Meta-analysismentioning

confidence: 99%

“…Cohen's kappa statistic (κ), therefore, is usually conducted to measure the agreement in this type of evaluation [55,56]. However, only 4 studies used κ to evaluate the diagnosis using ELISA in comparison to RBT [4,5,20,40]. In those studies, the estimated values of κ were between 0.229 and 0.38; indicating that the agreement was fair [55, .…”

Section: Plos Onementioning

confidence: 99%

Brucellosis in food-producing animals in Mosul, Iraq: A systematic review and meta-analysis

Dahl

2020

PLoS ONE

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Brucellosis is an endemic disease in food-producing animals in Mosul, Iraq. The objectives of the study reported here were: (i) to identify and assess the evidence and knowledge gaps in published studies that have examined brucellosis in different food-producing animals in Mosul, Iraq; using systematic review approach, and (ii) to quantify the seroprevalence of brucellosis in the city using meta-analysis approach. Google Scholar was used as a search engine to track pertinent peer-reviewed research reports. The search was conducted on November 24, 2019. Keywords used were: brucella, animal, Mosul, Iraq. Peer-reviewed published studies, MSc theses, and PhD dissertations written in Arabic or English were included. Duplicate records were removed, and the screening process was conducted at three levels: titles, abstracts, and full-text articles. Identified studies that have reported the seroprevalence of brucellosis were included in a meta-analysis to calculate an overall prevalence. A total of 214 records were initially identified. Seventeen research reports were added from personal contact and qualified articles' references list. Thirty six articles were qualified for review after removing 35 duplicate records, 155 titles, 11 abstracts, and 5 full text articles. Seventeen studies reported the prevalence of brucellosis, 11 studies assessed different serological tests for diagnosis of brucellosis, 9 studies isolated Brucella spp. from animal specimens and/or animal products, and 4 studies assessed vaccination procedures against brucellosis. The overall seroprevalence of brucellosis in food-producing animals in Mosul over a period of 40 years was 14.14%, including 14.46% for sheep, 12.99% for goats, 11.69% for cattle, and 22.64% for buffalo. The study concluded that the disease is evident in the city with increasing trends over the years, buffalo shows high seroprevalence, the degree of agreement of Rose-Bengal test as a screening test is fair compared to more accurate serological tests such as ELISA; and the disease constitutes a public health concern in the city. Additional studies are important to identify the overlooked predisposing factors, estimate the abortion rate attributable to brucellosis in food-producing animals, and evaluate efficacy of vaccination programs in reducing the prevalence of brucellosis and/or abortion rate.

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“…Indeed, those reports confirm that ELISA can detect latent infections with brucellosis at the early stages in calves (23,24). Also, it has been reported that indirect ELISA is considered an efficient diagnostic tool with high sensitivity, and this test is more sensitive than Rose Bengal and complement fixation tests (25,26). The Rose Bengal test uses a bacterial suspension of the whole cells of smooth strains of Brucella spp.…”

Section: Discussionmentioning

confidence: 89%

Serological detection of the latent infection of Brucellosis in calves in Mosul city, Iraq

Hassan¹,

Hasan²,

Abdulrazzaq³

et al. 2022

IJVS

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Brucellosis is a widespread and endemic disease in the Middle East and Mediterranean regions, and it has significant public health and livestock industry concerns. It can affect a wide range of mammalian hosts, including humans. This study aimed to determine the seroprevalence of the latent infection of brucellosis in calves; Rose Bengal and indirect ELISA tests were conducted for this purpose. The serum samples were collected from 184 local calves of both sexes aged between 1 to 8 months (92 males and 92 females) from different regions of Mosul city. The current study revealed that all samples tested negative with the Rose Bengal test, whereas, in indirect ELISA 5.9%, the samples were against Brucella species. The seroprevalence of Brucella antibodies showed no statistical differences between males and females, which was 5.4 and 6.5%, respectively. This study concluded that the indirect ELISA test showed high diagnostic efficacy in detecting anti-Brucella antibodies in young calves.

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Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul city

Cited by 3 publications

References 0 publications

The prevalence of brucellosis of farm animals using serum- and milk- ELISA test in Al- Najaf province

The prevalence of brucellosis of farm animals using serum- and milk- ELISA test in Al- Najaf province

Brucellosis in food-producing animals in Mosul, Iraq: A systematic review and meta-analysis

Serological detection of the latent infection of Brucellosis in calves in Mosul city, Iraq

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