Comparison between reflectance confocal microscopy and 2‐photon microscopy in early detection of cutaneous radiation injury in a mouse model in vivo

Jang, Won Hyuk; Kwon, Sang Hoon; Shim, Sehwan; Jang, Won‐Suk; Myung, Jae Kyung; Yang, Sejung; Park, Sunhoo; Kim, Ki Hean

doi:10.1002/jbio.201700337

Cited by 5 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Earlier work with other optical techniques, such as reflectance confocal microscopy (RCM) and 2-photon microscopy (TPM) have also shown promise in predicting latent skin injuries following irradiation. Endpoints included changes in cellular structure and morphology of the epidermis and skin-resident glands [ 140 ]. In use since at least the early 2000’s, thermal imaging of skin to assess severity of burns has been a viable option and important tool available to physicians [ 49 ].…”

Section: Current Researchmentioning

confidence: 99%

Cutaneous and local radiation injuries

et al. 2022

View full text Add to dashboard Cite

The threat of a large-scale radiological or nuclear (R/N) incident looms in the present-day climate, as noted most recently in an editorial in Scientific American (March 2021). These large-scale incidents are infrequent but affect large numbers of people. Smaller-scale R/N incidents occur more often, affecting smaller numbers of people. There is more awareness of acute radiation syndrome (ARS) in the medical community; however, ionising radiation-induced injuries to the skin are much less understood. This article will provide an overview of radiation-induced injuries to the skin, deeper tissues, and organs. The history and nomenclature; types and causes of injuries; pathophysiology; evaluation and diagnosis; current medical management; and current research of the evaluation and management are presented. Cutaneous radiation injuries (CRI) or local radiation injuries (LRI) may lead to cutaneous radiation syndrome, a sub-syndrome of ARS. These injuries may occur from exposure to radioactive particles suspended in the environment (air, soil, water) after a nuclear detonation or an improvised nuclear detonation (IND), a nuclear power plant incident, or an encounter with a radioactive dispersal or exposure device. These incidents may also result in a radiation-combined injury; a chemical, thermal, or traumatic injury, with radiation exposure. Skin injuries from medical diagnostic and therapeutic imaging, medical misadministration of nuclear medicine or radiotherapy, occupational exposures (including research) to radioactive sources are more common but are not the focus of this manuscript. Diagnosis and evaluation of injuries are based on the scenario, clinical picture, and dosimetry, and may be assisted through advanced imaging techniques. Research-based multidisciplinary therapies, both in the laboratory and clinical trial environments, hold promise for future medical management. Great progress is being made in recognising the extent of injuries, understanding their pathophysiology, as well as diagnosis and management; however, research gaps still exist.

show abstract

Section: Current Researchmentioning

confidence: 99%

Cutaneous and local radiation injuries

et al. 2022

View full text Add to dashboard Cite

show abstract

“…A heating plate (Chamlide TR, Live cell Instrument, Korea) was used to maintain the body temperature at 37°C during the anesthesia. Custom-made holders were used for in vivo imaging of the mouse cornea and ear to minimize breathing motion during imaging [25]. Moxifloxacin ophthalmic solution (Vigamox, Alcon Laboratories, Fort Worth, US, 12.4 mM) was used to label tissues prior TP imaging.…”

Section: Sample Preparationmentioning

confidence: 99%

High-speed combined reflectance confocal and moxifloxacin based two-photon microscopy

Kim¹,

Hoan²,

Oh³

et al. 2020

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

Reflectance confocal microscopy (RCM) is a non-invasive high-resolution optical imaging technique used in clinical settings as a diagnostic method. However, RCM has limited diagnostic ability by providing non-specific morphological information only based on reflection contrast. Various multimodal imaging techniques have been developed to compensate the limitations of RCM, but multimodal techniques are often slow in imaging speed compared to RCM alone. In this report, we combined RCM with moxifloxacin based two-photon microscopy (TPM) for high-speed multimodal imaging. Moxifloxacin based TPM used clinically compatible moxifloxacin for cell labeling and could do non-invasive cellular imaging at 30 frames/s together with RCM. Performance of the combined microscopy was characterized in the imaging of mouse skin and cornea, in vivo. Detail tissue microstructures including cells, extra-cellular matrix (ECM), and vasculature were visualized. The combined microscopy was applied to human skin cancer specimens, and both cells and ECM in the skin cancer and normal skin regions were visualized at high imaging speeds. The combined microscopy can be useful in the clinical applications of RCM by providing multiple contrasts.

show abstract

Advances in Microscopy

Mandal

Paul

2022

Automation and Basic Techniques in Medical Microbiology

View full text Add to dashboard Cite

Comparison between reflectance confocal microscopy and 2‐photon microscopy in early detection of cutaneous radiation injury in a mouse model in vivo

Cited by 5 publications

References 22 publications

Cutaneous and local radiation injuries

Cutaneous and local radiation injuries

High-speed combined reflectance confocal and moxifloxacin based two-photon microscopy

Advances in Microscopy

Contact Info

Product

Resources

About