2015
DOI: 10.1016/j.bjhh.2015.08.003
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Comparison between qualitative and real-time polymerase chain reaction to evaluate minimal residual disease in children with acute lymphoblastic leukemia

Abstract: IntroductionMinimal residual disease is an important independent prognostic factor that can identify poor responders among patients with acute lymphoblastic leukemia.ObjectiveThe aim of this study was to analyze minimal residual disease using immunoglobulin (Ig) and T-cell receptor (TCR) gene rearrangements by conventional polymerase chain reaction followed by homo-heteroduplex analysis and to compare this with real-time polymerase chain reaction at the end of the induction period in children with acute lympho… Show more

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Cited by 4 publications
(4 citation statements)
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References 28 publications
(42 reference statements)
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“…Alternatively, some authors have proposed the detection of Ig/TCR rearrangements by conventional PCR using consensus primers and homo/heteroduplex analysis, despite its lower analytical sensitivity, considering that this approach allows identification of patients with greater residual tumor burden, and then at high risk of relapse. 22 , 27 , 49 , 50 , 78 , 79 , 87 , 88 …”
Section: Discussionmentioning
confidence: 99%
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“…Alternatively, some authors have proposed the detection of Ig/TCR rearrangements by conventional PCR using consensus primers and homo/heteroduplex analysis, despite its lower analytical sensitivity, considering that this approach allows identification of patients with greater residual tumor burden, and then at high risk of relapse. 22 , 27 , 49 , 50 , 78 , 79 , 87 , 88 …”
Section: Discussionmentioning
confidence: 99%
“…Therefore, rearrangements of the immunoglobulin heavy chain gene (IgH), light chain kappa (IgK), TCR delta (TCRD), TCR gamma (TCRG), TCR beta (TCRB) and light chain lambda (IgL) may be detected at different frequencies in ALL of B and T-cell lineages. 11 , 17 , 19 , 35 , 37 , 41 , 62 , 68 , 72 79 …”
Section: Analysis Of Clonal Rearrangements Of the Ig And Tcr Genes Bymentioning
confidence: 99%
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“…In this issue of the Revista Brazileira de Hematology e Hemoterapy, Paula et al 15 compared MRD monitoring using Ig and TCR gene rearrangements by conventional PCR followed by homo-heteroduplex analysis with clone-specific probes to RQ-PCR at the end of induction in 44 children with ALL. According to RQ-PCR MRD cut-off points established by the GBTLI-2009 protocol, the agreement between the two methods was 40% for B lineage ALL and 100% for T-cell ALL.…”
mentioning
confidence: 99%