Comparison between prostate specific antigen and acid phosphatase for detection of semen in vaginal swabs from raped women

Peonim, Vichan; Worasuwannarak, Wisarn; Sujirachato, K; Teerakamchai, Somsri; Srisont, Smith; Udnoon, Jitta; Chudoung, Ubon

doi:10.1016/j.jflm.2013.06.009

Cited by 10 publications

(4 citation statements)

References 23 publications

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“…Hence, the detection of PSA and ACP on semen-stained under wares could be indicative of an incidence of sexual intercourse. This finding concurred with a similar study by Peonim et al 19 on the utilization of seminal biomarkers in determining the occurrence of sexual intercourse.…”

Section: Resultssupporting

confidence: 93%

Concept of Dried Seminal Biochemical Parameters in Substantiating Male Infidelity

Agoro,

Eri

2023

Asian J. Biol. Sci

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Background and Objective: Infidelity is the desecration of a couple's vows on sexual serenity and individuality leading to psychological damage, post-traumatic stress disorder, family breakdown and at times murder. Solutions to infidelity are multifaceted of which early detection with evidence could be of great help. This study was therefore designed to find an alternative approach to discovering a scientific means of detecting the occurrence of sexual intercourse which is the fulcrum of infidelity. Materials and Methods:The under wares of the subjects recruited for the study were collected after voluntarily enrolling in the study. The study was categorized into four groups, no-sex male (NSM), sex group (S), sex bath wash (SBW) and sex bath and no wash (SBNW). Concentrations or activities of seminal zinc, magnesium, inorganic phosphate, prostate-specific antigen (PSA) and acid phosphatase (ACP) were analyzed using WHO-approved methods after the elution of the semen-stained under wares in distilled water. One-way ANOVA (Post hoc-LSD) on SPSS (version 21) statistical software was used for the data analysis. Results: The findings of the study showed a significant increase in concentrations or activities of PSA and ACP when compared amongst the groups, whereas the seminal electrolytes were not significant. In a similar vein, comparisons of other parameters such as seminal magnesium, inorganic phosphate and zinc exhibited non-significant differences between the various studied groups. Conclusion:The concentrations of PSA and ACP could be utilized as an adjunct investigative tool in affirming the occurrence of sexual intercourse and infidelity in particular.

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Section: Resultssupporting

confidence: 93%

Concept of Dried Seminal Biochemical Parameters in Substantiating Male Infidelity

Agoro,

Eri

2023

Asian J. Biol. Sci

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“…Traditionally, an observed cut‐off time of 2 min for recording a positive response has been used by many laboratories, with response time at 2 min or longer often considered to be more indicative of a vaginal acid phosphatase (VAP) response. There are competing objectives of sensitivity and specificity with some researchers arguing that times of up to 10 min or longer (Lewis, Jones, Baxter, Siemieniuk, & Talbot, ; Redhead & Brown, ) should be considered, while others suggest reducing the time to as little as 15 s would increase the specificity and reduce the likelihood of false positives (Peonim et al, ).…”

Section: Forensic Tests For Semenmentioning

confidence: 99%

The characteristics of seminal fluid and the forensic tests available to identify it

Gamblin

Morgan-Smith

2019

WIREs Forensic Science

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Semen body fluid identification remains a critical aspect of the forensic investigation of alleged sexual assaults. Over the years, as advancements have been made in the area of forensic science, several different methods for semen identification have been utilized. It is not unusual for several methods of semen identification to be incorporated into a single sexual assault investigation. Most methods of semen identification are based on either detecting enzymatic activities or are targeted at specific cells or antigens. Other methods have evolved utilizing fluorescence or protein expression. Presumptive tests are often used to screen or locate semen staining. Further confirmatory tests can be used to identify the staining as semen and finally the donor of the stain can be further identified through DNA analysis. For routine application of these methods, they need to be specific, sensitive, cost effective, easy to use, and nondestructive. Although new techniques are being researched, it appears that traditional methods of semen identification remain the most widely applied in forensic casework. This article is categorized under: Forensic Biology > Body Fluid Identification

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“…Although the test is simple, cheap, and rapid there can be issues with reproducibility due to temperature variation at the scene, causing changes in evaporation of the sample [22]. The acid phosphatase (AP) test for semen has been in routine use as early as the 1940s [23], but due to its relatively high falsepositive rate [24] it is unable to be used to as a confirmatory test and has recently been sidelined in favour of immunoassay approaches [25]. A considerable advantage of chemical tests is their low pertest cost, but this is often at the expense of specificity and sensitivity.…”

Section: Chemical Testingmentioning

confidence: 99%

Field-based detection of biological samples for forensic analysis: Established techniques, novel tools, and future innovations

Morrison

Watts

Hobbs

et al. 2018

Forensic Science International

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Field based forensic tests commonly provide information on the presence and identity of biological stains and can also support the identification of species. Such information can support downstream processing of forensic samples and generate rapid intelligence. These approaches have traditionally used chemical and immunological techniques to elicit the result but some are known to suffer from a lack of specificity and sensitivity. The last 10 years has seen the development of field-based genetic profiling systems, with specific focus on moving the mainstay of forensic genetic analysis, namely STR profiling, out of the laboratory and into the hands of the non-laboratory user. In doing so it is now possible for enforcement officers to generate a crime scene DNA profile which can then be matched to a reference or database profile. The introduction of these novel genetic platforms also allows for further development of new molecular assays aimed at answering the more traditional questions relating to body fluid identity and species detection. The current drive for field-based molecular tools is in response to the needs of the criminal justice system and enforcement agencies, and promises a step-change in how forensic evidence is processed. However, the adoption of such systems by the law enforcement community does not represent a new strategy in the way forensic science has integrated previous novel approaches. Nor do they automatically represent a threat to the quality control and assurance practices that are central to the field. This review examines the historical need and subsequent research and developmental breakthroughs in field-based forensic analysis over the past two decades with particular focus on genetic methods Emerging technologies from a range of scientific fields that have potential applications in forensic analysis at the crime scene are identified and associated issues that arise from the shift from laboratory into operational field use are discussed.

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Comparison between prostate specific antigen and acid phosphatase for detection of semen in vaginal swabs from raped women

Cited by 10 publications

References 23 publications

Concept of Dried Seminal Biochemical Parameters in Substantiating Male Infidelity

Concept of Dried Seminal Biochemical Parameters in Substantiating Male Infidelity

The characteristics of seminal fluid and the forensic tests available to identify it

Field-based detection of biological samples for forensic analysis: Established techniques, novel tools, and future innovations

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