Comparison between placental and skeletal muscle ECM: <i>in vivo</i> implantation

Carvalho, Carlos Augusto Brandão de; Leonel, Luciano; Canada, Rafael R.; Barreto, Rodrigo da Silva Nunes; Maria, Durvanei Augusto; Sol, Mariano del; Miglino, María Angélica; Lobo, Sonja Ellen

doi:10.1080/03008207.2020.1834540

Cited by 7 publications

(6 citation statements)

References 48 publications

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“…23 Promising results have also been reported using decellularized human placenta xenografts in animal models for tissue regeneration. [24][25][26] In the current study, we hypothesized that decellularized placental sponges (DPS) could provide an excellent naturally derived matrix platform and composition biomimicking the natural bone tissue for coculture of macrophages and AD-MSCs and promote the AD-MSCs differentiation toward the osteogenic lineages.…”

Section: Introductionmentioning

confidence: 99%

Coculture of adipose‐derived mesenchymal stem cells/macrophages on decellularized placental sponge promotes differentiation into the osteogenic lineage

et al. 2022

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Background Several factors like three‐dimensional microstructure, growth factors, cytokines, cell–cell communication, and coculture with functional cells can affect the stem cells behavior and differentiation. The purpose of this study was to investigate the potential of decellularized placental sponge as adipose‐derived mesenchymal stem cells (AD‐MSCs) and macrophage coculture systems, and guiding the osteogenic differentiation of stem cells. Methods The decellularized placental sponge (DPS) was fabricated, and its mechanical characteristics were evaluated using degradation assay, swelling rate, and pore size determination. Its structure was also investigated using hematoxylin and eosin staining and scanning electron microscopy. Mouse peritoneal macrophages and AD‐MSCs were isolated and characterized. The differentiation potential of AD‐MSCs co‐cultured with macrophages was evaluated by RT‐qPCR of osteogenic genes on the surface of DPS. The in vivo biocompatibility of DPS was determined by subcutaneous implantation of scaffold and histological evaluations of the implanted site. Results The DPS had 67% porosity with an average pore size of 238 μm. The in vitro degradation assay showed around 25% weight loss during 30 days in PBS. The swelling rate was around 50% during 72 h. The coculture of AD‐MSCs/macrophages on the DPS showed a significant upregulation of four differentiation osteogenic lineage genes in AD‐MSCs on days 14 and 21 and a significantly higher mineralization rate than the groups without DPS. Subcutaneous implantation of DPS showed in vivo biocompatibility of scaffold during 28 days follow‐up. Conclusions Our findings suggest the decellularized placental sponge as an excellent bone substitute providing a naturally derived matrix substrate with biostructure close to the natural bone that guided differentiation of stem cells toward bone cells and a promising coculture substrate for crosstalk of macrophage and mesenchymal stem cells in vitro.

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Section: Introductionmentioning

confidence: 99%

Coculture of adipose‐derived mesenchymal stem cells/macrophages on decellularized placental sponge promotes differentiation into the osteogenic lineage

et al. 2022

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“…Despite their emerging potential in regenerative approaches, decellularized allogeneic and xenogeneic ECM scaffolds may cause an aggressive inflammatory response when implanted. To combat this, a recent study compared decellularized canine placentas and murine skeletal muscles as scaffolds for regenerating skeletal muscles in a rat model [ 39 ]. Muscle pockets were created at the posterior limbs of male Wistar rats, into which the muscle and placenta derived ECM were implanted.…”

Section: Survey Of Engineered Cell–ecm–materials Interactions In Musc...mentioning

confidence: 99%

“…Muscle pockets were created at the posterior limbs of male Wistar rats, into which the muscle and placenta derived ECM were implanted. Three days after implantation, a higher percentage of proliferative cells (PCNA + ) was seen in placenta-derived matrices compared to muscle derived matrices [ 39 ]. Additionally, higher percentages of CD163 +high macrophages were observed in the muscle derived ECM group, whereas CD163 +low macrophages were found at higher percentages in the placenta-derived ECM group three- and fifteen-days post implantation.…”

Section: Survey Of Engineered Cell–ecm–materials Interactions In Musc...mentioning

confidence: 99%

Engineering Cell–ECM–Material Interactions for Musculoskeletal Regeneration

2023

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The extracellular microenvironment regulates many of the mechanical and biochemical cues that direct musculoskeletal development and are involved in musculoskeletal disease. The extracellular matrix (ECM) is a main component of this microenvironment. Tissue engineered approaches towards regenerating muscle, cartilage, tendon, and bone target the ECM because it supplies critical signals for regenerating musculoskeletal tissues. Engineered ECM–material scaffolds that mimic key mechanical and biochemical components of the ECM are of particular interest in musculoskeletal tissue engineering. Such materials are biocompatible, can be fabricated to have desirable mechanical and biochemical properties, and can be further chemically or genetically modified to support cell differentiation or halt degenerative disease progression. In this review, we survey how engineered approaches using natural and ECM-derived materials and scaffold systems can harness the unique characteristics of the ECM to support musculoskeletal tissue regeneration, with a focus on skeletal muscle, cartilage, tendon, and bone. We summarize the strengths of current approaches and look towards a future of materials and culture systems with engineered and highly tailored cell–ECM–material interactions to drive musculoskeletal tissue restoration. The works highlighted in this review strongly support the continued exploration of ECM and other engineered materials as tools to control cell fate and make large-scale musculoskeletal regeneration a reality.

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“…Stem cells have the unique ability to differentiate into different tissue types, but they generally require specialized treatments to induce this differentiation into a particular cell type. The specific make-up of the ECM is unique to each tissue type, and the ECM has been shown to have the potential to induce and enhance the differentiation of stem cells towards the phenotype associated with the tissue source of the ECM [ 14 , 56 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 ].…”

Section: Structure and Properties Of Ecmmentioning

confidence: 99%

Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

McInnes

Möser

Chen

2022

JFB

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The multidisciplinary fields of tissue engineering and regenerative medicine have the potential to revolutionize the practise of medicine through the abilities to repair, regenerate, or replace tissues and organs with functional engineered constructs. To this end, tissue engineering combines scaffolding materials with cells and biologically active molecules into constructs with the appropriate structures and properties for tissue/organ regeneration, where scaffolding materials and biomolecules are the keys to mimic the native extracellular matrix (ECM). For this, one emerging way is to decellularize the native ECM into the materials suitable for, directly or in combination with other materials, creating functional constructs. Over the past decade, decellularized ECM (or dECM) has greatly facilitated the advance of tissue engineering and regenerative medicine, while being challenged in many ways. This article reviews the recent development of dECM for tissue engineering and regenerative medicine, with a focus on the preparation of dECM along with its influence on cell culture, the modification of dECM for use as a scaffolding material, and the novel techniques and emerging trends in processing dECM into functional constructs. We highlight the success of dECM and constructs in the in vitro, in vivo, and clinical applications and further identify the key issues and challenges involved, along with a discussion of future research directions.

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Comparison between placental and skeletal muscle ECM: in vivo implantation

Cited by 7 publications

References 48 publications

Coculture of adipose‐derived mesenchymal stem cells/macrophages on decellularized placental sponge promotes differentiation into the osteogenic lineage

Coculture of adipose‐derived mesenchymal stem cells/macrophages on decellularized placental sponge promotes differentiation into the osteogenic lineage

Engineering Cell–ECM–Material Interactions for Musculoskeletal Regeneration

Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

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