Comparison Between NaCl Tolerance Response and Acclimation to Cold Temperature in Shewanella putrefaciens

Leblanc, Laurence; Leboeuf, Céline; Leroi, Françoise; Hartke, Axel; Auffray, Yanick

doi:10.1007/s00284-002-3837-z

Cited by 31 publications

(17 citation statements)

References 15 publications

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“…Conversely, exposure to higher NaCl salt concentrations in some foods before cooling can also adapt cells to grow at low temperatures due to induction of cspA and cspD gene functions in response to NaCl stress. A similar phenomenon was recently described for the spoilage bacterium Shewanella putrefaciens (22) and for an lmo1078 gene transposon deletion mutant of L. monocytogenes (6). A mutant in this gene, which encodes a putative UDP-glucose phosphorylase enzyme, also exhibited cold and NaCl salt stress sensitivity.…”

Section: Discussionsupporting

confidence: 79%

Role of Cold Shock Proteins in Growth of Listeria monocytogenes under Cold and Osmotic Stress Conditions

Schmid

Klumpp

Raimann

et al. 2009

Appl Environ Microbiol

180

186

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The gram-positive bacterium Listeria monocytogenes is a food-borne pathogen of both public health and food safety significance. It possesses three small, highly homologous protein members of the cold shock protein (Csp) family. We used gene expression analysis and a set of mutants with single, double, and triple deletions of the csp genes to evaluate the roles of CspA, CspB, and CspD in the cold and osmotic (NaCl) stress adaptation responses of L. monocytogenes. All three Csps are dispensable for growth at optimal temperature (37°C). These proteins are, however, required for efficient cold and osmotic stress tolerance of this bacterium. The hierarchies of their functional importance differ, depending on the environmental stress conditions: CspA>CspD>CspB in response to cold stress versus CspD>CspA/CspB in response to NaCl salt osmotic stress. The fact that Csps are promoting L. monocytogenes adaptation against both cold and NaCl stress has significant implications in view of practical food microbial control measures. The combined or sequential exposure of L. monocytogenes cells to these two stresses in food environments might inadvertently induce cross-protection responses.

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Section: Discussionsupporting

confidence: 79%

Role of Cold Shock Proteins in Growth of Listeria monocytogenes under Cold and Osmotic Stress Conditions

Schmid

Klumpp

Raimann

et al. 2009

Appl Environ Microbiol

180

186

View full text Add to dashboard Cite

show abstract

“…A previous study also revealed that the expression of a homolog to the Escherichia coli Ahp is induced by osmotic shock in Staphylococcus aureus [34]. Similarly, the expression of Ahp in Shewanella putrefaciens is accompanied by accumulation of the corresponding transcript under NaCl stress [35] and the activity of the alkyl hydroperoxidase enzyme is dependent on salt concentration [36]. Collectively, the results from these studies indicate that expression of Ahps in general is upregulated not only by oxidative factors but also by other stresses, such as drought and salinity.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a salt-induced DhAHP, a gene coding for alkyl hydroperoxide reductase, from the extremely halophilic yeast Debaryomyces hansenii

Chao¹,

Yen²,

Ku³

2009

BMC Microbiol

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BackgroundDebaryomyces hansenii is one of the most salt tolerant species of yeast and has become a model organism for the study of tolerance mechanisms against salinity. The goal of this study was to identify key upregulated genes that are involved in its adaptation to high salinity.ResultsBy using forward subtractive hybridization we have cloned and sequenced DhAHP from D. hansenii that is significantly upregulated during salinity stress. DhAHP is orthologous to the alkly hydroperoxide reductase of the peroxiredoxin gene family, which catalyzes the reduction of peroxides at the expense of thiol compounds. The full-lengthed cDNA of DhAHP has 674 bp of nucleotide and contains a 516 bp open reading frame (ORF) encoding a deduced protein of 172 amino acid residues (18.3 kDa). D. hansenii Ahp is a cytosolic protein that belongs to the Ahp of the 1-Cys type peroxiredoxins. Phylogentically, the DhAhp and Candida albicans Ahp11 (Swiss-Prot: Q5AF44) share a common ancestry but show divergent evolution. Silence of its expression in D. hansenii by RNAi resulted in decreased tolerance to salt whereas overexpression of DhAHP in D. hansenii and the salt-sensitive yeasts Saccharomyces cereviasiae and Pichia methanolica conferred a higher tolerance with a reduced level of reactive oxygen species.ConclusionIn conclusion, for the first time our study has identified alkly hydroperoxide reductase as a key protein involved in the salt tolerance of the extremely halophilic D. hansenii. Apparently, this enzyme plays a multi-functional role in the yeast's adaptation to salinity; it serves as a peroxidase in scavenging reactive oxygen species, as a molecular chaperone in protecting essential proteins from denaturation, and as a redox sensor in regulating H2O2-mediated cell defense signaling.

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“…Shewanella is able to survive in a wide range of environments, including spoiled food and deep-sea and freshwater lake sediment as well as animals’ and patients’ blood and intestines [3]–[11]. Therefore, Shewanella has been suggested to be a good candidate model for studying how microorganisms respond to environmental stresses, such as osmolarity, temperature, and pH [12]–[16].…”

Section: Introductionmentioning

confidence: 99%

Time Course Transcriptome Changes in Shewanella algae in Response to Salt Stress

Wang

Yin

et al. 2014

PLoS ONE

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Shewanella algae, which produces tetrodotoxin and exists in various seafoods, can cause human diseases, such as spondylodiscitis and bloody diarrhea. In the present study, we focused on the temporal, dynamic process in salt-stressed S. algae by monitoring the gene transcript levels at different time points after high salt exposure. Transcript changes in amino acid metabolism, carbohydrate metabolism, energy metabolism, membrane transport, regulatory functions, and cellular signaling were found to be important for the high salt response in S. algae. The most common strategies used by bacteria to survive and grow in high salt environments, such as Na+ efflux, K+ uptake, glutamate transport and biosynthesis, and the accumulation of compatible solutes, were also observed in S. algae. In particular, genes involved in peptidoglycan biosynthesis and DNA repair were highly and steadily up-regulated, accompanied by rapid and instantaneous enhancement of the transcription of large- and small-ribosome subunits, which suggested that the structural changes in the cell wall and some stressful responses occurred in S. algae. Furthermore, the transcription of genes involved in the tricarboxylic acid (TCA) cycle and the glycolytic pathway was decreased, whereas the transcription of genes involved in anaerobic respiration was increased. These results, demonstrating the multi-pathway reactions of S. algae in response to salt stress, increase our understanding of the microbial stress response mechanisms.

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Comparison Between NaCl Tolerance Response and Acclimation to Cold Temperature in Shewanella putrefaciens

Cited by 31 publications

References 15 publications

Role of Cold Shock Proteins in Growth of Listeria monocytogenes under Cold and Osmotic Stress Conditions

Role of Cold Shock Proteins in Growth of Listeria monocytogenes under Cold and Osmotic Stress Conditions

Characterization of a salt-induced DhAHP, a gene coding for alkyl hydroperoxide reductase, from the extremely halophilic yeast Debaryomyces hansenii

Time Course Transcriptome Changes in Shewanella algae in Response to Salt Stress

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