Comparison between liquid chromatography-tandem mass spectrometry and immunoassay methods for measurement of plasma 25 (OH) vitamin D

Abstract

Objectives Vitamin D is one of the major hormones involved in the metabolism of calcium (Ca) and phosphorus (P). In the present study, we aimed to determine the analytical performance of the immunoassay method used for determining plasma 25-hydroxyvitamin D [25(OH)D] levels in routine clinical practice in laboratories. Methods Venous blood samples were collected from 156 patients for the comparisons and were analyzed with Siemens ADVIA Centaur XPT, the Roche Cobas 6,000’s module e601, Abbott Architect i2000, and the liquid chromatography with tandem mass spectrometry (LC-MS/MS). Results The four methods were analyzed and compared through the Passing-Bablok regression for 25(OH)D, and the highest correlation was found at LC-MS/MS and Cobas 6,000’s module e601 (r=0.799), LC-MS/MS/Abbott Architect i2000, and LC-MS/MS/Siemens ADVIA Centaur XPT as r=0.736, 0.721, respectively. The correlation coefficient was found between Abbott Architect i2000 with Roche Cobas e601 and Siemens ADVIA Centaur XPT as r=0.934 and r=0.907, respectively. Also, the correlation coefficient was found between Roche Cobas e601 and Siemens ADVIA Centaur XPT as r=0.906. Conclusions The Roche Cobas assay showed better performance, compared with the other assays. Based on our findings, the chemiluminescence methods in automated systems seem to be expedient.

Assessment of the vitamin D status and its determinants in young healthy students from Palestine

“…The calculation of vitamin D intake from diet-based 24-h recalls is inexact due to over-and underreporting of food intake, inaccurate information on vitamin D concentrations in food databases as well as variations due to cooking methods, thus our focus on serum 25-(OH)D concentrations analysed using CMIA is a strength. However, we note that CMIA measurement may overestimate 25-(OH)D at high concentrations compared with high-pressure liquid chromatography combined with mass spectrometric analyses (LC-MS/MS) (54,55) . Nevertheless, because the 25-(OH)D concentrations measured in this study were generally low, especially for women an overestimation of the 25-(OH)D concentrations is unlikely.…”

“…Nevertheless, because the 25-(OH)D concentrations measured in this study were generally low, especially for women an overestimation of the 25-(OH)D concentrations is unlikely. Rather, both methods provide sufficient sensitivity to detect a vitamin D deficiency ( 24 , 55 ) .…”