Comparison between ki-67 index and S-phase fraction on fine-needle aspiration samples from breast carcinoma

Bozzetti, Cecilia; Nizzoli, Rita; Camisa, Roberta; Guazzi, Annamaria; Ceci, Guido; Cocconi, Giorgio; Mazzini, Giuliano; Naldi, Nadia

doi:10.1002/(sici)1097-0142(19971025)81:5<287::aid-cncr6>3.0.co;2-k

Cited by 14 publications

(1 citation statement)

References 34 publications

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“…In addition, we sought to determine the best cell proliferation method for predicting disease outcome, comparing distinct markers in the same series of patients. [9][10][11][12][13][14][15] We tested Ki-67, a nuclear antigen present in all active phases of the cell cycle (G1, S, G2, and mitosis (M)), 16 which is a valuable indicator of tumour proliferation and prognosis in patients with breast cancer. [17][18][19][20] The immunohistochemical Ki-67 index has the technical advantage, in relation to flow cytometry, of allowing the morphological evaluation of proliferating cell populations.…”

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Prognostic comparative study of S-phase fraction and Ki-67 index in breast carcinoma

Pinto¹,

André²,

Pereira³

et al. 2001

Journal of Clinical Pathology

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Aims-To investigate the prognostic value of recently proposed flow cytometric S-phase fraction (SPF) variables (average SPF and SPF tertiles) compared with conventional SPF, and to compare the one with the best predictive value with the immunohistochemical Ki-67 index in breast carcinoma. Methods-A short term follow up study (median, 39.6 months) of a large series of patients (n = 306) was conducted. DNA ploidy was analysed on fresh/frozen tumour samples by flow cytometry, and the SPF was calculated from the DNA histogram using an algorithm. The Ki-67 index was assessed on paraYn wax embedded material by immunohistochemistry (cut oV point, 10%). The two methods were compared by means of statistics, and the prognostic significance of both in relation to disease free survival (DFS) and overall survival (OS) was determined. Conclusions-Flow cytometric SPF is a better prognosticator than the Ki-67 index, but only SPF variables applied in the whole series show potential clinical usefulness. (J Clin Pathol 2001;54:543-549) Results-SPF

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confidence: 99%

Prognostic comparative study of S-phase fraction and Ki-67 index in breast carcinoma

Pinto¹,

André²,

Pereira³

et al. 2001

Journal of Clinical Pathology

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Value of Ki67 immunostain in identification of malignancy in serous effusions

1999

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The distinction between malignant and benign serous effusions continues to be a challenging and a frequent problem to cytopathologists. Recently, immunostains employing various antibodies have improved the diagnostic accuracy of malignant effusions. We investigated the usefulness of Ki67 (MIB1) antigen immunostaining in the evaluation and diagnosis of malignant serous effusions. Cell block sections from a total of 54 cases of serous effusions cytologically diagnosed as malignant (28), suspicious (6), and benign (20) were immunostained with MIB1 monoclonal antibody to the Ki67 nuclear proliferation antigen according to the avidin‐biotin immunoperoxidase method. The patients were 30 women and 24 men with an average age of 58 yr. Ki67 (MIB1) immunostain labeling index (LI) values were higher than 20% in 23 of 28 (82%) cytologically malignant, in 3 of 6 (50%) suspicious, and in 1 of 20 (5%) benign/reactive. Further investigation revealed histologic, radiologic, and/or clinical evidence of malignancy in the 3 suspicious (but not in the benign/reactive) cases with Ki67 LI values higher than 20%. Correlation between Ki67 LI (>20%) and cytologic effusion type (benign, suspicious, or malignant) was statistically significant (P < 0.0001). Ki67 immunostaining has value as an adjunct testing to cytomorphology and other immunostains in distinguishing benign from malignant effusions. The addition of Ki67 immunostaining to conventional cytology appears more sensitive than cytomorphology alone and may assist in arriving at accurate diagnoses in suspicious cases with inconclusive cytomorphologic features. Diagn. Cytopathol. 1999;20:24–28. © 1999 Wiley‐Liss, Inc.

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Histochemistry in Advanced Cytometry: From Fluorochromes to Mass Probes

Mazzini

Danova

2022

Methods in Molecular Biology

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Comparison between ki-67 index and S-phase fraction on fine-needle aspiration samples from breast carcinoma

Cited by 14 publications

References 34 publications

Prognostic comparative study of S-phase fraction and Ki-67 index in breast carcinoma

Prognostic comparative study of S-phase fraction and Ki-67 index in breast carcinoma

Value of Ki67 immunostain in identification of malignancy in serous effusions

Histochemistry in Advanced Cytometry: From Fluorochromes to Mass Probes

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