Comparison Between Free Amino Acid Levels in Plasma Deproteinated with Picric Acid and with Sulfosalicylic Acid.

Block, Walter D.; Markovs, Mara E.; Steele, Betty F.

doi:10.3181/00379727-122-31333

Cited by 58 publications

(16 citation statements)

References 2 publications

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“…Plasma amino acids were measured by an automatic amino acid analyzer (lLC-300, Japan Electric Optical Laboratory, Tokyo, Japan) in samples that had been deproteinized by sulfosalicylic acid. 16 ) Statistics. An analysis of variance and the multiple comparison procedure of Tukey were adopted to test the statistical differences.…”

Section: Methodsmentioning

confidence: 99%

Interaction of Dietary Protein and Fat on Plasma Cholesterol and Amino Acid Levels, Fatty Acid Desaturation, and Prostacyclin Production in Exogenous Hypercholesterolemic Rats

Ikeda

Imaizumi

Sugano

1993

Bioscience, Biotechnology, and Biochemistry

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Section: Methodsmentioning

confidence: 99%

Interaction of Dietary Protein and Fat on Plasma Cholesterol and Amino Acid Levels, Fatty Acid Desaturation, and Prostacyclin Production in Exogenous Hypercholesterolemic Rats

Ikeda

Imaizumi

Sugano

1993

Bioscience, Biotechnology, and Biochemistry

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“…Content of free amino acids in plasma deproteinized with sulfosalicylic acid (13) was determined by an automated two column ion-exchange technique using the Beckman model 121 (Beckman Instruments, Inc., Palo Alto, Calif.) amino acid analyzer (14). Urinary excretion of urea was measured (15) on a 24 h sample of urine collected during the day before the study of splanchnic metabolism.…”

Section: Methodsmentioning

confidence: 99%

Mechanism of the Hypolipemic Effect of Clofibrate in Postabsorptive Man

Wolfe¹,

Kane²,

Havel³

et al. 1973

J. Clin. Invest.

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A B S T R A C T Splanchnic metabolism of triglycerides and other major substrates was studied in the postabsorptive state in normotriglyceridemic and hypertriglyceridemic human subjects wh}o received A g of clofibrate four times daily for 3 wNk. Transport in blood plasma of triglycerides produced in the splanchnic region was quantified by three methods: (a) measurement of the transsplanchnic gradient of '4C-labeled triglycerides during constant intravenous infusion of [1-"C]palmitate (b) chemical measurement of the transsplanchnic gradient in concenltration of triglycerides of very low density lipoproteins; and (c) determination of clearance of 14C-labeled triglycerides in extrasplanchnic tissues. The first method measures only triglycerides derived from free fatty acids and the last two measure total splanchnic production. In hypertriglyceridemic subjects treated with clofibrate, average rates of total splanchulic production of triglycerides and productioni from free fatty acids were the same as those of conmparable untreated subjects despite a consistent fall in plasma triglyceride levels. The hvpotriglyceridemic effect of the drug was therefore accompanied by improved disposal of triglycerides in extrasplanchnic tissues. In treated normotriglyceridemic subjects, unlike their untreated counterparts, total splanchnic production wvas significantly higlher than prodluction from free fatty acids.

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“…Lactate (13) and pyruvate (14) were measured in whole blood by enzymatic techniques. Individual amino acids were determined by the automated ion-exchange chromatographic technique (15) on heparinized plasma deproteinized with sulfosalicyclic acid (16). Amino acid analysis was performed on the basal arterial and hepatic venous samples and on the specimens obtained at the conclusion of the high dose (20 min), and low dose (45 min) glucose infusions.…”

Section: Blood Analysesmentioning

confidence: 99%

Influence of Endogenous Insulin Secretion on Splanchnic Glucose and Amino Acid Metabolism in Man

Felig¹,

Wahren²

1971

J. Clin. Invest.

273

117

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A B S T R A C T Splanchnic exchange of glucose, 20 individual amino acids, lactate, and pyruvate was studied in normal subjects in the postabsorptive state and after stimulation of endogenous insulin secretion by infusion of glucose at two dose levels. In the basal state, mean splanchnic glucose production was 3.4 mg/kg per min. A net uptake of lactate, pyruvate, and nine amino acids was observed, with alanine accounting for half of the total splanchnic-amino acid extraction.Infusion of glucose at 25 mg/kg per min for 20 min resulted in a fivefold increase in arterial insulin levels and in reversal of splanchnic glucose balance to a net uptake. Splanchnic uptake of alanine, glycine, phenylalanine, lactate, and pyruvate fell by 30-60% due to a reduction in fractional extraction of these substrates, inasmuch as their arterial concentrations did not decline.Administration of glucose at 2 mg/kg per min for 45 min resulted in a 19 mg/100 ml increase in arterial glucose concentration and a doubling of arterial insulin levels. Despite the small increment in insulin, hepatic glucose production fell by 85%. Splanchnic exchange of amino acids, lactate, and pyruvate was unaltered. Estimated total glucose utilization during the infusion was no greater than in the basal state, indicating lack of stimulation of peripheral glucose uptake.It is concluded that: (a) inhibition of hepatic glucose production associated with glucose infusion and large increments in insulin levels occurs in the absence of a decrease in the concentration of circulating gluconeogenic substrate, suggesting an hepatic rather than peripheral effect; (b) the liver is the primary target organ whereby glucose homeostasis is achieved with Dr. Felig is a Teaching and Research Scholar of the American College of Physicians.

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Comparison Between Free Amino Acid Levels in Plasma Deproteinated with Picric Acid and with Sulfosalicylic Acid.

Cited by 58 publications

References 2 publications

Interaction of Dietary Protein and Fat on Plasma Cholesterol and Amino Acid Levels, Fatty Acid Desaturation, and Prostacyclin Production in Exogenous Hypercholesterolemic Rats

Interaction of Dietary Protein and Fat on Plasma Cholesterol and Amino Acid Levels, Fatty Acid Desaturation, and Prostacyclin Production in Exogenous Hypercholesterolemic Rats

Mechanism of the Hypolipemic Effect of Clofibrate in Postabsorptive Man

Influence of Endogenous Insulin Secretion on Splanchnic Glucose and Amino Acid Metabolism in Man

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