Comparison between Emerging Q Fever in French Guiana and Endemic Q fever in Marseille, France

Edouard, Sophie; Mahamat, Aba; Demar, Magalie; Abboud, Philippe; Djossou, Félix; Raoult, Didier

doi:10.4269/ajtmh.13-0164

Cited by 37 publications

(43 citation statements)

References 25 publications

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“…Therefore, there is a real outbreak in Cayenne linked to a unique and exclusive clone, which likely explains the distinct clinical manifestation observed with the Cayenne "geotype". 12 Recently in a collaborative work done with Bernard Davoust, we were able, after a hundred samples tested from animals, to identify the three-toed sloth (Fig. 1) as the likely source of the Q Fever outbreak in Cayenne in French Guiana.…”

Section: The Outbreak In Guyanamentioning

confidence: 97%

Recent advances in the study of Q fever epidemiology, diagnosis and management

Million

Raoult

2015

Journal of Infection

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112

110

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Section: The Outbreak In Guyanamentioning

confidence: 97%

Recent advances in the study of Q fever epidemiology, diagnosis and management

Million

Raoult

2015

Journal of Infection

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112

110

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“…Whole lungs and whole spleens were homogenized in PBS, and DNA from lung suspensions (200 l), spleen suspensions (100 l), and blood (200 l) was extracted using a QIAamp tissue kit (Qiagen) in a 100-l final volume. Quantitative real-time PCR (qPCR) was performed using the CFX96 qPCR detection system (Bio-Rad, France), and carried out with 5 l of DNA extract and specific primers and a probe targeting a fragment of the C. burnetii 16S DNA gene, as previously described (22), and IS1111 (23,24). Negative controls consisted of DNA extracted from the organs and blood of PBS-challenged mice.…”

Section: Methodsmentioning

confidence: 99%

Mouse Model of Coxiella burnetii Aerosolization

et al. 2016

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cCoxiella burnetii is mainly transmitted by aerosols and is responsible for multiple-organ lesions. Animal models have shown C. burnetii pathogenicity, but long-term outcomes still need to be clarified. We used a whole-body aerosol inhalation exposure system to mimic the natural route of infection in immunocompetent (BALB/c) and severe combined immunodeficient (SCID) mice. After an initial lung inoculum of 10 4 C. burnetii cells/lung, the outcome, serological response, hematological disorders, and deep organ lesions were described up to 3 months postinfection. C. burnetii-specific PCR, anti-C. burnetii immunohistochemistry, and fluorescent in situ hybridization (FISH) targeting C. burnetii-specific 16S rRNA completed the detection of the bacterium in the tissues. In BALB/c mice, a thrombocytopenia and lymphopenia were first observed, prior to evidence of C. burnetii replication. In all SCID mouse organs, DNA copies increased to higher levels over time than in BALB/c ones. Clinical signs of discomfort appeared in SCID mice, so follow-up had to be shortened to 2 months in this group. At this stage, all animals presented bone, cervical, and heart lesions. The presence of C. burnetii could be attested in situ for all organs sampled using immunohistochemistry and FISH. This mouse model described C. burnetii Nine Mile strain spread using aerosolization in a way that corroborates the pathogenicity of Q fever described in humans and completes previously published data in mouse models. C. burnetii infection occurring after aerosolization in mice thus seems to be a useful tool to compare the pathogenicity of different strains of C. burnetii.

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“…38 This study showed that age and sex of patients did not significantly differ between the two areas. Patients from Cayenne had a significantly higher prevalence of fever (97%) and pneumonia (83%) than did patients from metropolitan France (81% and 8%, respectively).…”

Section: Clinical Featuresmentioning

confidence: 72%

“…This study also showed that the serologic profile of Q fever patients from Cayenne was unusual; significantly higher phase I IgG titers were observed. 38 The main clinical presentation of Q fever in French Guiana is pneumonia, and it has an exceptionally high prevalence. Patients with pneumonia have a good outcome despite a more severe initial clinical presentation.…”

Section: Clinical Featuresmentioning

confidence: 99%

Q Fever in French Guiana

Eldin¹,

Mahamat²,

Demar³

et al. 2014

The American Society of Tropical Medicine and Hygiene

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Abstract. Coxiella burnetii, the causative agent of Q fever, is present worldwide. Recent studies have shown that this bacterium is an emerging pathogen in French Guiana and has a high prevalence (24% of community-acquired pneumonia). In this review, we focus on the peculiar epidemiology of Q fever in French Guiana. We place it in the context of the epidemiology of the disease in the surrounding countries of South America. We also review the clinical features of Q fever in this region, which has severe initial presentation but low mortality rates. These characteristics seem to be linked to a unique genotype (genotype 17). Finally, we discuss the issue of the animal reservoir of C. burnetii in French Guiana, which is still unknown. Further studies are necessary to identify this reservoir. Identification of this reservoir will improve the understanding of the Q fever epidemic in French Guiana and will provide new tools to control this public health problem.

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Comparison between Emerging Q Fever in French Guiana and Endemic Q fever in Marseille, France

Cited by 37 publications

References 25 publications

Recent advances in the study of Q fever epidemiology, diagnosis and management

Recent advances in the study of Q fever epidemiology, diagnosis and management

Mouse Model of Coxiella burnetii Aerosolization

Q Fever in French Guiana

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