Comparison between electrochemical ELISA and spectrophotometric ELISA for the detection of dentine sialophosphoprotein for root resorption

Sha, Hailiang; Bai, Yuxing; Li, Song; Wang, Xiofeng; Yin, Yajiang

doi:10.1016/j.ajodo.2013.09.008

Cited by 17 publications

(21 citation statements)

References 15 publications

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“…The detection limit reached using our device is comparable with values reported in previously published studies describing electrochemical ELISA carried out with different devices and antigen/antibody pairs. For example, Nemiroski et al 2 reported a LOD of 20 ng mL −1 for Pf HRP2, Bhimji et al 8 reported a LOD of 1 ng mL −1 for HIV-1 antibodies, Sun et al 55 reported a LOD of 0.5 ng mL −1 for cucumber mosaic virus, Liu et al 4 reported a LOD of 0.02 ng mL −1 for α-fetoprotein, and Sha et al 3 reported a LOD of 0.5 pg mL −1 for the detection of dentine sialophosphoprotein. Electrochemical Sandwich ELISA for Detection of Pf HRP2.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Folding Analytical Devices for Electrochemical ELISA in Hydrophobic R^H Paper

et al. 2014

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This work describes a device for electrochemical enzyme-linked immunosorbent assay (ELISA) designed for low-resource settings and diagnostics at the point of care. The device is fabricated entirely in hydrophobic paper, produced by silanization of paper with decyl trichlorosilane, and comprises two zones separated by a central crease: an embossed microwell, on the surface of which the antigen or antibody immobilization and recognition events occur, and a detection zone where the electrodes are printed. The two zones are brought in contact by folding the device along this central crease; the analytical signal is recorded from the folded configuration. Two proof-of-concept applications, an electrochemical direct ELISA for the detection of rabbit IgG as a model antigen in buffer and an electrochemical sandwich ELISA for the detection of malarial histidine-rich protein from Plasmodium falciparum (Pf HRP2) in spiked human serum, show the versatility of this device. The limit of detection of the electrochemical sandwich ELISA for the quantification of Pf HRP2 in spiked human serum was 4 ng mL −1 (10 2 pmol L −1 ), a value within the range of clinically relevant concentrations.

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Section: ■ Results and Discussionmentioning

confidence: 99%

“…We connected the paper device to the potentiostat using a reusable magnetic PDMS holder ( Figure S4). The PDMS holder consisted of two parts (a top part, 3 × 1.1 × 0.5 cm 3 , and a Figure 1. Fabrication of paper-based electrochemical devices.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Folding Analytical Devices for Electrochemical ELISA in Hydrophobic R^H Paper

et al. 2014

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“…Moreover, it is possible that our samples were either too diluted for subsequent analysis or the traditional ELISA method was not sufficiently sensitive to detect DSP in GCF. Recently, Sha et al 21 recommended a novel method that combines ELISA with electrochemical analysis of GCF samples for the detection of dentin proteins. The authors state that the high sensitivity of this method extends the detection limit of the assay and could be a new way to measure dentin proteins in minute samples such as GCF.…”

Section: Discussionmentioning

confidence: 99%

Immunoassay analysis of proteins in gingival crevicular fluid samples from resorbing teeth

Rody

Wijegunasinghe

Holliday

et al. 2015

The Angle Orthodontist

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Objective: To carry out an immunoassay analysis of biomarkers expressed in gingival crevicular fluid (GCF) with the main goal of finding a useful diagnostic pattern to distinguish between resorbing deciduous teeth and nonresorbing controls. Materials and Methods: A split-mouth design was used in this study with a total of 22 GCF samples collected from 11 patients in the mixed dentition. For each child, one deciduous molar with radiographic evidence of root resorption was used as the test tooth whereas the contralateral first permanent molar with formed roots was used as the control tooth. Samples were processed with immunoassays using a panel of selected biomarkers including interleukin-1 beta (IL-1b), interleukin-1 receptor antagonist (IL-1RA), nuclear factor kappa B ligand (RANKL), osteoprotegerin (OPG), matrix metalloproteinase-9 (MMP-9), and dentin sialoprotein (DSP). Results: There were no statistically significant differences in levels of IL-1b, OPG, and MMP-9 between test and control sites (P . .05). IL-1RA was the only biomarker to show a significant down-regulation (P 5 .04) in GCF samples collected from resorbing teeth. RANKL data showed a heavily skewed distribution and was deemed unreliable. Only one deciduous GCF sample had detectable levels of DSP; therefore, no further statistical calculation was applicable because of the limited amount of data for this biomarker. Conclusions: This study indicated that IL1-RA is down-regulated in GCF from resorbing primary molars, thus suggesting this cytokine as a potential analyte to be included in a panel that can discriminate between resorbing and nonresorbing teeth. (Angle Orthod. 2016;86:187-192.)

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“…In recent years, ELISA combined with electrochemical detection has extended the limit of detection by a factor of 10 with small biological samples [33] yet there has been no appreciable decrease in cost. In this work, the classical sandwich ELISA was modified to detect antigen by using an electrochemical technique (Fig.…”

Section: Electrochemical Sandwich Elisamentioning

confidence: 99%

Yeast dual-affinity biobricks: Progress towards renewable whole-cell biosensors

Venkatesh

Sun

Brickner

et al. 2015

Biosensors and Bioelectronics

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Abstract:Point-of-care (POC) diagnostic biosensors offer a promising solution to improve healthcare, not only in developed parts of the world, but also in resource limited areas that lack adequate medical infrastructure and trained technicians. However, in remote and resource limited settings, cost and storage of traditional POC immunoassays often limit actual deployment. Synthetically engineered biological components ("BioBricks") provide an avenue to reduce costs and simplify assay procedures. In this article, the design and development of an ultra-low cost, whole-cell "renewable" capture reagent for use in POC diagnostic applications is described. Yeast cells were genetically modified to display both single chain variable fragment (scFv) antibodies and goldbinding peptide (GBP) on their surfaces for simple one step enrichment and surface functionalization. Electrochemical impedance spectroscopy (EIS) and fluorescent imaging were used to verify and characterize the binding of cells to gold electrodes. A complete electrochemical detection assay was then performed on screen-printed electrodes fixed with yeast displaying scFv directed to Salmonella outer membrane protein D (OmpD). Electrochemical assays were optimized and cross-validated with established fluorescence techniques. Nanomolar detection limits were observed for both formats.

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Comparison between electrochemical ELISA and spectrophotometric ELISA for the detection of dentine sialophosphoprotein for root resorption

Cited by 17 publications

References 15 publications

Folding Analytical Devices for Electrochemical ELISA in Hydrophobic R^H Paper

Folding Analytical Devices for Electrochemical ELISA in Hydrophobic R^H Paper

Immunoassay analysis of proteins in gingival crevicular fluid samples from resorbing teeth

Yeast dual-affinity biobricks: Progress towards renewable whole-cell biosensors

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Comparison between electrochemical ELISA and spectrophotometric ELISA for the detection of dentine sialophosphoprotein for root resorption

Cited by 17 publications

References 15 publications

Folding Analytical Devices for Electrochemical ELISA in Hydrophobic RH Paper

Folding Analytical Devices for Electrochemical ELISA in Hydrophobic RH Paper

Immunoassay analysis of proteins in gingival crevicular fluid samples from resorbing teeth

Yeast dual-affinity biobricks: Progress towards renewable whole-cell biosensors

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Product

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Folding Analytical Devices for Electrochemical ELISA in Hydrophobic R^H Paper

Folding Analytical Devices for Electrochemical ELISA in Hydrophobic R^H Paper