Comparison between ectoderm-conditioned medium and fibronectin in their effects on chondrogenesis by limb bud mesenchymal cells

Zanetti, Nina C.; Dress, Virginia M.; Solursh, Michael

doi:10.1016/0012-1606(90)90307-5

Cited by 24 publications

(9 citation statements)

References 52 publications

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“…However, the role of the ECM in shape-dependent growth control could not be directly determined in these studies because it was not possible to control the chemistry of the substrates or to rule out changes in cell sensitivity to different growth factors in the medium. More recent studies carried out under more defined conditions confirm that the ability of various ECM molecules, including laminin, types I, III, IV and V collagens, and different fibronectin isoforms, to stimulate growth correlates with their ability to promote cell spreading in several cell types [49][50][51][52][53][54] , although the nature of the integrin subtype activated by the ECM ligands may also be important 19 . Similar tight coupling between cell shape and proliferation has been shown by preventing cell spreading through reducing the density of the ECM molecule coated on otherwise non-adhesive dishes 45,55,56 or using a peptide containing the aminoacid sequence RGD, a cell-binding peptide from fibronectin 57 .…”

Section: Shape and Tension Dependencementioning

confidence: 99%

The structural and mechanical complexity of cell-growth control

1999

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Tight control of cell proliferation is required to ensure normal tissue patterning and prevent cancer formation. The analysis of cultured cells has led to an explosion in our understanding of the molecules that trigger growth and mediate cell-cycle progression. However, the mechanism by which the local growth differentials that drive morphogenesis are established and maintained still remains unknown. Here we review recent work that reveals the importance of cell binding to the extracellular matrix, and associated changes in cell shape and cytoskeletal tension, to the spatial control of cell-cycle progression. These findings change the paradigm of cell-growth control, by placing our understanding of molecular signalling cascades in the context of the structural and mechanical complexity of living tissues.

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Section: Shape and Tension Dependencementioning

confidence: 99%

The structural and mechanical complexity of cell-growth control

1999

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“…1A). Early studies have shown that treating limb mesenchymal cells with cytochalasin D, which depolymerizes actin filaments, stimulates chondrogenic differentiation and blocks the anti-chondrogenic effects of ectoderm-conditioned medium (Zanetti et al, 1990;Zanetti and Solursh, 1984). More recently, synthetic substrates that were designed to control cell shape have provided evidence in support of the idea that restricting cell spreading promotes differentiation of mesenchymal stem cells towards chondrocytes (Gao et al, 2010).…”

Section: Cell Shape and Chondrogenic Differentiationmentioning

confidence: 99%

Fibronectin and stem cell differentiation – lessons from chondrogenesis

Singh

Schwarzbauer

2012

Journal of Cell Science

168

156

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SummaryThe extracellular matrix (ECM) is an intricate network of proteins that surrounds cells and has a central role in establishing an environment that is conducive to tissue-specific cell functions. In the case of stem cells, this environment is the stem cell niche, where ECM signals participate in cell fate decisions. In this Commentary, we describe how changes in ECM composition and mechanical properties can affect cell shape and stem cell differentiation. Using chondrogenic differentiation as a model, we examine the changes in the ECM that occur before and during mesenchymal stem cell differentiation. In particular, we focus on the main ECM protein fibronectin, its temporal expression pattern during chondrogenic differentiation, its potential effects on functions of differentiating chondrocytes, and how its interactions with other ECM components might affect cartilage development. Finally, we discuss data that support the possibility that the fibronectin matrix has an instructive role in directing cells through the condensation, proliferation and/or differentiation stages of cartilage formation.

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“…Fibronectin, a player for cell–matrix interaction, promotes attachment and spreading of cells over the substratum, which seems to be necessary for condensation but inhibits chondrogenesis (Swalla & Solursh ; Zanetti et al . ; Gehris et al . ).…”

Section: Growth and Differentiation Of Cartilage And Bone In A Long Bonementioning

confidence: 99%

Growth and differentiation of a long bone in limb development, repair and regeneration

et al. 2014

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Repair from traumatic bone fracture is a complex process that includes mechanisms of bone development and bone homeostasis. Thus, elucidation of the cellular/molecular basis of bone formation in skeletal development would provide valuable information on fracture repair and would lead to successful skeletal regeneration after limb amputation, which never occurs in mammals. Elucidation of the basis of epimorphic limb regeneration in amphibians would also provide insights into skeletal regeneration in mammals, since the epimorphic regeneration enables an amputated limb to re-develop the three-dimensional structure of bones. In the processes of bone development, repair and regeneration, growth of the bone is achieved through several events including not only cell proliferation but also aggregation of mesenchymal cells, enlargement of cells, deposition and accumulation of extracellular matrix, and bone remodeling.

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Comparison between ectoderm-conditioned medium and fibronectin in their effects on chondrogenesis by limb bud mesenchymal cells

Cited by 24 publications

References 52 publications

The structural and mechanical complexity of cell-growth control

The structural and mechanical complexity of cell-growth control

Fibronectin and stem cell differentiation – lessons from chondrogenesis

Growth and differentiation of a long bone in limb development, repair and regeneration

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