Comparison between Echinococcus granulosus sensu stricto (G1) and E. canadensis (G6) mitochondrial genes (cox1 and nad1) and their related protein models using experimental and bioinformatics analysis

Sadjjadi, Seyed Mahmoud; Ebrahimipour, Mohammad; Sadjjadi, Fatemeh Sadat

doi:10.1016/j.compbiolchem.2019.02.002

Cited by 17 publications

(19 citation statements)

References 34 publications

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“…The G6 genotype, which has already been reported as a main causative agent of brain CE in Iran (16)(17); is widespread in Birjand, Eastern Iran (21).…”

Section: Discussionmentioning

confidence: 98%

“…Different aspects of the disease including the molecular perspective of human and animal CE is useful for control programs. Re-cently many researchers have utilized molecular tools to access the information about parasite genotypic diversity (16)(17)(18)(19)(20)(21)26). Several genes including both coding and non-coding regions have used for intra-species differentiation; however, mitochondrial markers such as cox1 have shown great power in genetic discrimination (25)(26)(27)(28).…”

Section: Discussionmentioning

confidence: 99%

“…Five genotypes of E. granulosus including E. granulosus sensu stricto (G1-G3 complex), E. ortleppi (G5), and E. canadensis (G6) have been reported from animal and/or human in Iran (4,(15)(16)(17)(18)(19)(20)(21).…”

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confidence: 99%

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Genetic Diversity of Hepatic/Non-Hepatic Cystic Echinococ-cosis in Baqiyatallah Hospital, Tehran, Iran

Neysi

Mohammadzadeh

Sadjjadi

et al. 2020

IJPA

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Background: Cystic echinococcosis (CE) is a worldwide zoonotic helminthic disease caused by the larval stage of Echinococcus granulosus. The infection is particularly important in terms of economic and medico-veterinary aspects in endemic areas including Iran. Considering the possibility of organ-tropism in E. granulosus strains, the present study was aimed to identify the genotypes of E. granulosus in different organs involved in patients, undergone surgery in Baqiyatallah Hospital, Tehran, Iran from 2005-2015. Methods: Overall, 29 formalin-fixed paraffin-embedded tissues (FFPT) from patients with histologically confirmed CE including liver (N: 14) lungs (N: 6) abdomen (N: 2), pancreas (N: 2) and each of spleen, gallbladder and, muscles (N: 1) plus unknown organs (N: 2) were used and genetically characterized using polymerase chain reaction, followed by partial sequencing of mitochondrial cytochrome c oxidase gene subunit 1(cox1) and analyzed. Results: Nineteen out of 29 isolates including liver (N: 6) lungs (N: 4) abdomen (N: 2), pancreas (N: 2) and each of spleen, gallbladder, and muscle (N: 1), unknown organs (N: 2) obtained from paraffin-embedded blocks of human CE created an acceptable sequence in two directions. All 19 isolates regardless of the organ involved were recognized as E. granulosus sensu stricto (G1). Conclusion: The sequence alignments of the isolates displayed two profiles. All sequenced samples showed E. granulosus sensu stricto (G1) with no organ-related genotype.

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“…The G6 genotype, which has already been reported as a main causative agent of brain CE in Iran (16)(17); is widespread in Birjand, Eastern Iran (21).…”

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Genetic Diversity of Hepatic/Non-Hepatic Cystic Echinococ-cosis in Baqiyatallah Hospital, Tehran, Iran

Neysi

Mohammadzadeh

Sadjjadi

et al. 2020

IJPA

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“…The protein sequences of the full-length, N-terminal domain and the C-terminal domain were, respectively, aligned using the MUSCLE online service. Next, the alignments were manually further adjusted using the BioEdit v7.0.0 software (Alzohairy, 2011), then the pairwise alignments for the sequence identities were analyzed using the Sequence Identity Matrix in the BioEdit software. The Jones, Taylor, and Thornton (JTT) amino acid substitution model was selected by the ModelGenerator program version 0.85.…”

Section: Phylogenetic Analysis and Homology Modelingmentioning

confidence: 99%

Biochemical Functions of Glutathione S-Transferase Family of Salix babylonica

Zhuge

Xiu

et al. 2020

Front. Plant Sci.

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Glutathione S-transferases (GSTs) are ubiquitous enzymes that are encoded by a large gene family, and they contribute to the detoxification of endogenous or xenobiotic compounds and oxidative stress metabolism in plants. Although the GSTs gene family has been reported in many land plants, our knowledge of the evolution and function of the willow GSTs is still limited. In this study, 22 full-length GST genes were cloned from Salix babylonica and divided into three classes based on the conserved domain analysis, phylogenetic tree and gene structure: tau, phi and DHAR. The tissue-specific expression patterns were substantially different among the tau and phi GSTs. The Salix GST proteins showed functional divergences in the substrate specificities, substrate activities and kinetic characteristics. The site-directed mutagenesis studies revealed that a single amino acid mutation (Ile/Val53→Thr53) resulted in the lowest activity of SbGSTU7 among the Salix GSTs. These results suggest that non-synonymous substitution of an amino acid at the putative glutathione-binding site may play an important role in the divergence of enzymatic functions of Salix GST family.

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“…Disease diagnosis requires different aspects of research including serological methods, imaging and clinical findings [15]. Consideration has been paid on the proteome assessment of the affected person to overcome the diagnostic complications [16]. Because of the parasite's genetic diversity its control in the endemic regions is challenging.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of the Mitochondrial Proteins Reveals Complex Structural and Functional Relationships in <em>Fasciola</em> Species

Ahmad¹,

Majeed²,

Ijaz³

et al. 2020

Preprint

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Mitochondria is a cellular source of energy, playing an essential role in cellular stress induced by environmental stimuli. The genetic diversity of mitochondrial genes involved in oxidative phosphorylation affects the production of cellular energy and regional adaptation to various ecological (climatic) pressures influencing amino acid sequences (variants of protein). However, a little is known about the combined effect of protein changes on cell-level metabolic alterations in simultaneous exposure to various environmental conditions, including mitochondrial dysfunction and oxidative stress induction. Present study was designed to address this issue by analyzing the mitochondrial proteins in Fasciola species including Cytochrome C oxidase (COX1, COX2, COX3 and CYTB) and NADH dehydrogenase (ND1, ND2, ND3, ND4, ND5 and ND6). Mitochondrial proteins were used for a detailed computational investigation using available standard bioinformatics tools to explore structural and functional relationships. Our analysis shows that the mitochondrial protein family of Fasciola species are extensively diversified in all species studied, showing an extending role in various biological processes The results showed that the protein of COX1 of F. hepatica, F. gigantica and F. jacksoni consist of 510, 513 and 517 amino acids respectively. The alignment of proteins showed that these proteins are conserved in the same regions at ten positions in COX and CYTB proteins while at twelve locations in NADH. Three dimensional structure of COX, CYTB and NADH proteins were compared and the differences in additional conserved and binding sites in COX and CYTB proteins as compared to NADH were found in three Fasciola species. These results, based on the amino acid diversity pattern, were used to identify sites in the enzyme and the variations in mitochondrial proteins among Fasciola species. This study provides valuable information for future experimental studies including identification of therapeutics, diagnostics and immunoprophylactic interests with novel mitochondrial proteins.

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Comparison between Echinococcus granulosus sensu stricto (G1) and E. canadensis (G6) mitochondrial genes (cox1 and nad1) and their related protein models using experimental and bioinformatics analysis

Cited by 17 publications

References 34 publications

Genetic Diversity of Hepatic/Non-Hepatic Cystic Echinococ-cosis in Baqiyatallah Hospital, Tehran, Iran

Genetic Diversity of Hepatic/Non-Hepatic Cystic Echinococ-cosis in Baqiyatallah Hospital, Tehran, Iran

Biochemical Functions of Glutathione S-Transferase Family of Salix babylonica

Comparative Analysis of the Mitochondrial Proteins Reveals Complex Structural and Functional Relationships in <em>Fasciola</em> Species

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