Comparison between E-test and CLSI broth microdilution method for antifungal susceptibility testing of Candida albicans oral isolates

Koga-Ito, Cristiane Yumi; Lyon, Juliana Pereira; Resende, Maria Aparecida de

doi:10.1590/s0036-46652008000100002

Cited by 22 publications

(13 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It can easily be used, on a large scale, by laboratories with few technological features. Other techniques such as disk diffusion, E-test, colorimetric methods are used; however, the broth dilution method is considered the standard practice due to its good reproducibility (Koga-Ito et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

In vitro evaluation of antifungal activity and interactive effect of Anadenanthera colubrina (Benth)

Nunes¹,

Viana²,

Rocha³

et al. 2015

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Anadenanthera colubrina (Benth) Brenan, a plant known in the Northeastern Region of Brazil as angico, is widely used in traditional folk medicine to treat respiratory and inflammatory diseases. This study aimed to evaluate the antifungal activity, determine the minimum inhibitory concentration (MIC), the minimum fungicidal concentration and the fungal kinetics (death curve) in addition to the interactive effect of the dry extract of angico in association with the antifungals fluconazole and nystatin against yeasts of the genus Candida. The dry extract was obtained by rotoevaporation. Tests for evaluation of antifungal activity, determination of the MIC and the MFC as well as the evaluation of the interactive effect with conventional antifungal were done by disk diffusion and microdilution technique. For the evaluation of the angico's effect on fungal growth, death curve was utilized. The results show the angico's antifungal potential in all of the strains tested, having MIC of 1.0 mg/mL. It was observed that the fungal kinetics of 2x MIC, MIC and ½ MIC had similar effects; 6 h was their best time after incubation. There was fungistatic activity reduction (2 log 10 UFC/mL) from the initial inoculum of 1.0 mg/mL. Interactive effect was not observed when used in association with nystatin, but showed synergistic effect when used with fluconazole. In these data, one can see that angico is a species rich in biological activity; being promising species, the isolation and detection of its bioactive compounds is necessary.

show abstract

Section: Discussionmentioning

confidence: 99%

In vitro evaluation of antifungal activity and interactive effect of Anadenanthera colubrina (Benth)

Nunes¹,

Viana²,

Rocha³

et al. 2015

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…E-test has been considered as an alternative to the reference broth microdilution method (CLSI) for its practicability and reproducibility [14]- [16]. It is easy, fast, and can be used by the gynecologist for increased reliability of prescription medication, especially in more complicated cases [15]- [17].…”

Section: Introductionmentioning

confidence: 99%

Susceptibility to Fluconazole and Ketoconazole of <i>Candida</i> spp. Isolated from Primary and Episodic Vulvovaginites by E-Test (São Paulo, SP, Brazil)

Boatto¹,

Girão²,

Francisco³

et al. 2016

OJOG

View full text Add to dashboard Cite

Purpose: To evaluate the profile of in vitro susceptibility of yeasts isolated from cases of primary and espisodic vulvovaginitis to two antifungal agents. Methods: 40 Candida isolates from episodes of vulvovaginal candidiasis were identified by classic methodologies. The susceptibility testing of the in vitro fluconazole and ketoconazole activity against the isolates was accessed by E-test. Results: C. albicans was the most common species identified in 70% of the occurrences followed by C. glabrata (20%), C. tropicalis (7.5%), and C. guilliermondii (2.5%). In the susceptibility profile to antifungal agents, 12.5% and 16.7% of the isolates obtained from primary and episodic vulvovaginal candidiasis were resistant to fluconazole, respectively. To ketoconazole, we found that 6.25% and 12.5% of the isolates respectively from primary vulvovaginal candidiasis (PVVC) and episodic vulvovaginal candidiasis (EVVC) had high MIC values. Conclusions: E-test is a reliable method for the susceptibility testing of Candida spp. due to its simplicity, reproducibility, and lack of specialized equipment. Resistant strains and non-albicans species were verified more in cases of EVVC than in PVVC. Clinical and mycological cure of patients with episodic vulvovaginal candidiasis or complicated cases occurred after prolonged treatment and sometimes with multiple antifungals use.

show abstract

“…Oral candidiasis is recently increasing due to old age, denture use, diabetes, systemic steroid and antibiotic use, pernicious anemia, malignancy, radiation therapy on the head and neck, and cell-mediated immunodeficiency 1 2 . With the increasing use of antifungal agents, several reports on antifungal resistance have been published 3 4 5 6 7 8 9 10 11 12 13 14 15 . Therefore, antifungal susceptibility testing becomes necessary for the effective treatment of oral candidiasis.…”

Section: Introductionmentioning

confidence: 99%

Antifungal Susceptibility Testing with Etest forCandidaSpecies Isolated from Patients with Oral Candidiasis

Song

Suh

et al. 2015

Ann Dermatol

View full text Add to dashboard Cite

BackgroundThe necessity of performing antifungal susceptibility tests is recently increasing because of frequent cases of oral candidiasis caused by antifungal-resistant Candida species. The Etest (BioMerieux, Marcy l'Etoile, France) is a rapid and easy-to-perform in vitro antifungal susceptibility test.ObjectiveThe purpose of this study was to determine the minimal inhibitory concentrations (MICs) of antifungal agents by using the Etest for Candida species isolated from patients with oral candidiasis.MethodsForty-seven clinical isolates of Candida species (39 isolates of Candida albicans, 5 isolates of C. glabrata, and 3 isolates of C. tropicalis) were tested along with a reference strain (C. albicans ATCC 90028). The MIC end points of the Etest for fluconazole, itraconazole, voriconazole, and amphotericin B susceptibility were read after the 24-hour incubation of each isolate on RPMI 1640 agar.ResultsAll Candida isolates were found susceptible to voriconazole and amphotericin B. However, all five isolates of C. glabrata were resistant to itraconazole, among which two isolates were also resistant to fluconazole.ConclusionThis study revealed that the Etest represented a simple and efficacious method for antifungal susceptibility testing of Candida species isolated from oral candidiasis patients. Therefore, voriconazole and amphotericin B should be recommended as effective alternatives for the treatment of oral candidiasis.

show abstract

Comparison between E-test and CLSI broth microdilution method for antifungal susceptibility testing of Candida albicans oral isolates

Cited by 22 publications

References 22 publications

In vitro evaluation of antifungal activity and interactive effect of Anadenanthera colubrina (Benth)

In vitro evaluation of antifungal activity and interactive effect of Anadenanthera colubrina (Benth)

Susceptibility to Fluconazole and Ketoconazole of <i>Candida</i> spp. Isolated from Primary and Episodic Vulvovaginites by E-Test (São Paulo, SP, Brazil)

Antifungal Susceptibility Testing with Etest forCandidaSpecies Isolated from Patients with Oral Candidiasis

Contact Info

Product

Resources

About

Comparison between E-test and CLSI broth microdilution method for antifungal susceptibility testing of Candida albicans oral isolates

Cited by 22 publications

References 22 publications

In vitro evaluation of antifungal activity and interactive effect of Anadenanthera colubrina (Benth)

In vitro evaluation of antifungal activity and interactive effect of Anadenanthera colubrina (Benth)

Susceptibility to Fluconazole and Ketoconazole of &lt;i&gt;Candida&lt;/i&gt; spp. Isolated from Primary and Episodic Vulvovaginites by E-Test (S&#227;o Paulo, SP, Brazil)

Antifungal Susceptibility Testing with Etest forCandidaSpecies Isolated from Patients with Oral Candidiasis

Contact Info

Product

Resources

About

Susceptibility to Fluconazole and Ketoconazole of <i>Candida</i> spp. Isolated from Primary and Episodic Vulvovaginites by E-Test (São Paulo, SP, Brazil)