Comparison between Chinese medical herb Pueraria lobata crude extract and its main isoflavone puerarin

Guerra, Maurizio; Speroni, Ester; Broccoli, Massimiliano; Cangini, Monica; Pasini, Patrizia; Minghetti, A.; Crespi‐Perellino, N.; Mirasoli, Mara; Cantelli‐Forti, Giorgio; Paolini, Moreno

doi:10.1016/s0024-3205(00)00885-7

Cited by 182 publications

(104 citation statements)

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“…The traditional Chinese medicine pueraria has been used to treat alcoholism for thousands of years. Recently, many observations demonstrate puerarin extracted from pueraria is antioxidative, can prevent injuries to cells due to lipid peroxidation, and can maintain the stability of the cell membrane structure by removing the oxyradicals from tissues and cells [4,6,12,13]. The protective effects of puerarin on cells are also related to calcium ion rivalry and b-receptor blockage [54].…”

Section: Discussionmentioning

confidence: 99%

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Preventive Effects of Puerarin on Alcohol-induced Osteonecrosis

Wang

Yin

et al. 2008

Clinical Orthopaedics &Amp; Related Research

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Alcohol can induce adipogenesis by bone marrow stromal cells and may cause osteonecrosis of the femoral head. Currently, there are no medications available to prevent alcohol-induced osteonecrosis. We hypothesized puerarin, a Chinese herbal medicine with antioxidative and antithrombotic effects, can prevent alcohol-induced adipogenesis and osteonecrosis. Both bone marrow stromal cells (in vitro) and mice (in vivo) were treated either with ethanol or with ethanol and puerarin, with an untreated group serving as a control. In the in vitro study, the number of adipocytes, contents of triglycerides, and levels of PPARc mRNA expression were decreased and alkaline phosphatase activity, contents of osteocalcin, and levels of osteocalcin mRNA expression were increased in cells treated with both alcohol and puerarin, compared with cells treated with alcohol only. In the in vivo study, marrow necrosis, fat cell hypertrophy and proliferation, thinner and sparse trabeculae, diminished hematopoiesis, and increased empty osteocyte lacunae in the subchondral region of the femoral head were observed in mice treated with alcohol. However, no such changes were seen in femoral heads of mice treated with alcohol and puerarin. The data suggest puerarin can inhibit adipogenic differentiation by bone marrow stromal cells both in vitro and in vivo and prevents alcohol-induced osteonecrosis in this model.

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Section: Discussionmentioning

confidence: 99%

“…Recently, it was found puerarin, extracted from pueraria, is antioxidative, antithrombotic, and decreases cell injuries secondary to lipid peroxidation by protecting the stability of the cell membrane [4,6,7,11,12,53,55]. The findings indicate puerarin may have the potential to prevent alcohol-induced ON.…”

Section: Introductionmentioning

confidence: 98%

Preventive Effects of Puerarin on Alcohol-induced Osteonecrosis

Wang

Yin

et al. 2008

Clinical Orthopaedics &Amp; Related Research

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“…17) NHF is consisted of Polygonati Rhizoma, Rehmanniae Radix Crudus, Salviae miltiorrhizae Radix, Puerariae Radix, Schizandrae Fructus, and Glycyrrhizae Radix. In traditional Oriental medicine, Polygonati Rhizoma, 18) Rehmanniae Radix Crudus, 19) Puerariae Radix 20) and Schizandrae Fructus 21) have been used mainly for the decrease of blood glucose. Traditionally, Salviae miltiorrhizae Radix has been widely used for the treatment of coronary heart disease, cerebrovascular disease, hepatitis, hepatocirrhosis, chronic renal failure, dysmenorrheal and neurasthetic insomnia.…”

Section: Fig 6 Histological Observations and Immunohistochemical Stmentioning

confidence: 99%

Anti-diabetic Effects of New Herbal Formula in Neonatally Streptozotocin-Induced Diabetic Rats

Kim

Lee

Kwon

et al. 2009

Biological & Pharmaceutical Bulletin

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The present study investigated the anti-diabetic effects of new herbal formula (NHF) consist of Polygonati Rhizoma, Rehmanniae Radix, Salviae miltiorrhizae Radix, Puerariae Radix, Schizandrae Fructus, Glycyrrhizae Radix in neonatal streptozotocin (nSTZ)-induced non-insulin-dependent diabetes mellitus (NIDDM) rats. Changes of food and water intakes, body weight, blood glucose, plasma insulin and immunohistochemical evaluation of insulin on pancreas, and mRNA expression of glucose transporter subtype-4 (GLUT-4) in skeletal muscle and hepatic phosphoenolpyruvate carboxykinase (PEPCK) by administration of NHF (300 mg/kg) were investigated. The nSTZ diabetic rats showed hyperglycemia, increases in food and water intake, loss of body weight gain and decrease of the number of insulin-positive cells and the size of b b-cells in pancreas and mRNA of GLUT-4 in soleus muscle and increase of hepatic PEPCK mRNA expression. Administration of NHF significantly decreased the blood glucose level, food and water intake and considerably increased the body weight of nSTZ diabetic rats. Also, NHF treatment significantly increased plasma insulin level and the number and size of insulinimmunoreactive cells in the pancreas of nSTZ diabetic rats. In addition, NHF treatment resulted in increased expression of the GLUT-4 mRNA in soleus muscle and in reduced expression of PEPCK mRNA in liver. These results provide possible mechanisms for the anti-diabetic effects of NHF, via a decrease of blood glucose level, an increase of insulin sensitivity, an increase of GLUT-4 gene expression and an attenuation of hepatic PEPCK gene expression. In conclusion, NHF may be useful for improving hyperglycemia and reducing the risk of diabetic complications.

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“…1) Puerariae radix is comprised of puerarin, daidzin, daidzein, genistein, and biochanin A; of these, daidzin and daidzein, the major active constituents of Puerariae radix extracts, are known to suppress ethanol intake. 19,20) In a previous study, the inhibitory effect of Puerariae flos on alcohol-induced apoptosis, which appears to act by inhibiting caspase-3 mRNA expression, in neuroblastoma cells was reported. 21) In the present study, Puerariae radix treatment increased the number of Fos-positive cells in the hippocampus of alcohol-intoxicated rats in a dose-dependent manner, while Puerariae radix exerted no significant effect on Fos expression in the hippocampus of normal rats.…”

Section: Discussionmentioning

confidence: 98%