Comparison between avian pathogenic (APEC) and avian faecal (AFEC) Escherichia coli isolated from different regions in Brazil

Silveira, Frank Ferreira; Maluta, Renato Pariz; Tiba, Monique Ribeiro; Paiva, Jacqueline Boldrin de; Guastalli, Elisabete Aparecida Lopes; Silveira, Wanderley Dias da

doi:10.1016/j.tvjl.2016.06.007

Cited by 12 publications

(14 citation statements)

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“…Intestinal pathogenic E. coli (IPEC) are causing diarrhoea syndromes and seldom colonize healthy humans whereas extraintestinal pathogenic E. coli (ExPEC) often innocuously colonize the gut but have the ability to cause severe infections such as meningitis or blood stream infections (Vila et al ., 2016). Recent studies compared the occurrence of certain virulence‐associated genes (VAGs) in avian pathogenic E. coli (APEC), uropathogenic E. coli (UPEC) and commensal E. coli from humans and broiler chickens (Kemmett et al ., 2013; de Carli et al ., 2015; van Hoek et al ., 2016; Paixao et al ., 2016; Silveira et al ., 2016). It turned out that certain VAGs (e.g.…”

Section: Introductionmentioning

confidence: 99%

Environmental adaptation and vertical dissemination of ESBL‐/pAmpC‐producing Escherichia coli in an integrated broiler production chain in the absence of an antibiotic treatment

Projahn

Daehre

Semmler

et al. 2018

Microbial Biotechnology

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SummaryHigh prevalence numbers of extended‐spectrum beta‐lactamase‐ (ESBL‐)/plasmid‐mediated AmpC beta‐lactamase‐ (pAmpC‐) producing Escherichia coli in broiler chicken and their distribution along the broiler production chain is an ongoing problem in food production. We, therefore, investigated resistant isolates along the broiler production chain to determine whether there is a constantly occurring direct vertical transmission of the ESBL‐/pAmpC‐producing E. coli from the parent flocks to their offspring or not. We, furthermore, analysed the isolates concerning the occurrence of virulence factors and their ability to form biofilms to estimate their potential to effectively colonize broiler chickens and/or persist and survive in the environment of the broiler production facilities. Using whole genome sequencing, we could show that ESBL‐/pAmpC‐producing E. coli were likely transferred in a step‐wise process along the broiler production chain but not directly from the parent flock to the fattening flock with every single batch of offspring chickens. Additionally, resistant E. coli strains showing an extraintestinal pathogenic genotype as well as high numbers of virulence‐associated genes including the production of curli fibres and cellulose have high capabilities to persist and spread in the broiler production chain.

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Section: Introductionmentioning

confidence: 99%

Environmental adaptation and vertical dissemination of ESBL‐/pAmpC‐producing Escherichia coli in an integrated broiler production chain in the absence of an antibiotic treatment

Projahn

Daehre

Semmler

et al. 2018

Microbial Biotechnology

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“…Moreover, many human and animal-source ExPEC isolates exhibit similar VAGs which may suggest that they represent zoonotic pathogens ( 10 ). What is more, previously described virulence factors associated with this disease do not always serve to distinguish APEC from commensal strains as they can be found in both groups ( 16 , 31 ). The genomes announced in this paper provide original information concerning sequences of extraintestinal pathogenic Escherichia coli strains (ExPEC) isolated from colibacillosis lesions.…”

Section: Discussionmentioning

confidence: 83%

“…( 12 ) reported five genes (iutA, hlyF, iss, iroN , and ompT ) to be the minimal predictors of pathogenic strains, and Silveira et al . ( 31 ) compared APEC and AFEC strains and revealed that a bigger set of genes might be needed to differentiate pathogenic from non-pathogenic bacteria well. Therefore, in this study, a set of 16 factors reported previously ( 7 , 12 , 14 , 26 , 28 ) as virulent for poultry were chosen and in silico analysis of 14 original APEC genomes with 16 strains regarded as APEC was performed and the results deposited in the NCBI database.…”

Section: Discussionmentioning

confidence: 99%

“…There are no clear predictors for the pathogenicity of the strains. They are very diversified according to geographic location and, to make it even more complicated, AFEC strains (avian faecal E. coli ) isolated from healthy birds can also contain certain genes coding virulence mechanisms ( 31 ). Moreover, the complexity of the molecular basis of pathogenesis makes the task of defining the exact APEC pathotype extremely difficult ( 4 , 19 ).…”

Section: Introductionmentioning

confidence: 99%

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In silico analysis of virulence associated genes in genomes of Escherichia coli strains causing colibacillosis in poultry

Kołsut¹,

Borówka

Marciniak

et al. 2017

Journal of Veterinary Research

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IntroductionColibacillosis – the most common disease of poultry, is caused mainly by avian pathogenic Escherichia coli (APEC). However, thus far, no pattern to the molecular basis of the pathogenicity of these bacteria has been established beyond dispute. In this study, genomes of APEC were investigated to ascribe importance and explore the distribution of 16 genes recognised as their virulence factors.Material and MethodsA total of 14 pathogenic for poultry E. coli strains were isolated, and their DNA was sequenced, assembled de novo, and annotated. Amino acid sequences from these bacteria and an additional 16 freely available APEC amino acid sequences were analysed with the DIFFIND tool to define their virulence factors.ResultsThe DIFFIND tool enabled quick, reliable, and convenient assessment of the differences between compared amino acid sequences from bacterial genomes. The presence of 16 protein sequences indicated as pathogenicity factors in poultry resulted in the generation of a heatmap which categorises genomes in terms of the existence and similarity of the analysed protein sequences.ConclusionThe proposed method of detection of virulence factors using the capabilities of the DIFFIND tool may be useful in the analysis of similarities of E. coli and other sequences deriving from bacteria. Phylogenetic analysis resulted in reliable segregation of 30 APEC strains into five main clusters containing various virulence associated genes (VAGs).

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“…Currently serotypes O1:K1, O2:K1 and O78:K80 are recognized as the most prevalent serotypes among more than 20 APEC subtypes identified in animal studies. However, despite the huge importance of APECs for bird health there are few studies searching for genes evolved in recombination and positive selection as well as specific virulence factors associated with traits and phenotypes [ 49 – 51 ]. More importantly, most the time these pathogenic strains are carrying plasmids that disseminate resistance to ampicillin, tetracycline, gentamicin, neomycin, sulfa trimethoprim, enrofloxacin and norfloxacin [ 5 , 58 ].…”

Section: Introductionmentioning

confidence: 99%

Novel biotechnological approaches for monitoring and immunization against resistant to antibiotics Escherichia coli and other pathogenic bacteria

Belizário

Sircili

2020

BMC Vet Res

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The application of next-generation molecular, biochemical and immunological methods for developing new vaccines, antimicrobial compounds, probiotics and prebiotics for zoonotic infection control has been fundamental to the understanding and preservation of the symbiotic relationship between animals and humans. With increasing rates of antibiotic use, resistant bacterial infections have become more difficult to diagnose, treat, and eradicate, thereby elevating the importance of surveillance and prevention programs. Effective surveillance relies on the availability of rapid, cost-effective methods to monitor pathogenic bacterial isolates. In this opinion article, we summarize the results of some research program initiatives for the improvement of live vaccines against avian enterotoxigenic Escherichia coli using virulence factor gene deletion and engineered vaccine vectors based on probiotics. We also describe methods for the detection of pathogenic bacterial strains in eco-environmental headspace and aerosols, as well as samples of animal and human breath, based on the composition of volatile organic compounds and fatty acid methyl esters. We explain how the introduction of these low-cost biotechnologies and protocols will provide the opportunity to enhance co-operation between networks of resistance surveillance programs and integrated routine workflows of veterinary and clinical public health microbiology laboratories.

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Comparison between avian pathogenic (APEC) and avian faecal (AFEC) Escherichia coli isolated from different regions in Brazil

Cited by 12 publications

References 10 publications

Environmental adaptation and vertical dissemination of ESBL‐/pAmpC‐producing Escherichia coli in an integrated broiler production chain in the absence of an antibiotic treatment

Environmental adaptation and vertical dissemination of ESBL‐/pAmpC‐producing Escherichia coli in an integrated broiler production chain in the absence of an antibiotic treatment

In silico analysis of virulence associated genes in genomes of Escherichia coli strains causing colibacillosis in poultry

Novel biotechnological approaches for monitoring and immunization against resistant to antibiotics Escherichia coli and other pathogenic bacteria

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