Comparison between Apicystis cryptica sp. n. and Apicystis bombi (Arthrogregarida, Apicomplexa): Gregarine parasites that cause fat body hypertrophism in bees

Schoonvaere, Karel; Brunain, Marleen; Baeke, Femke; Bruyne, Michiel De; Rycke, Riet De; Graaf, Dirk C. de

doi:10.1016/j.ejop.2020.125688

Cited by 8 publications

(4 citation statements)

References 38 publications

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“…(Table S7 and Figure S5). Finally, Apicystis parasites were identified as Apicystis cryptica (3 bees) and Apicystis bombi (1 bee) based on an earlier re‐evaluation of publicly available Apicystis sequences [as per Supplementary file 1 in Schoonvaere et al (2020)].…”

Section: Resultsmentioning

confidence: 99%

Microbiota and pathogens in an invasive bee: Megachile sculpturalis from native and invaded regions

Tuerlings

Hettiarachchi

Joossens

et al. 2023

Insect Molecular Biology

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The present study aimed to characterise the bacterial, fungal and parasite gut community of the invasive bee Megachile sculpturalis sampled from native (Japan) and invaded (USA and France) regions via 16S rRNA and ITS2 amplicon sequencing and PCR detection of bee microparasites. The bacterial and fungal gut microbiota communities in bees from invaded regions were highly similar and differed strongly from those obtained in Japan. Core amplicon sequence variants (ASVs) within each population represented environmental micro‐organisms commonly present in bee‐associated niches that likely provide beneficial functions to their host. Although the overall bacterial and fungal communities of the invasive M. sculpturalis in France and the co‐foraging native bees Anthidium florentinum and Halictus scabiosae, were significantly different, five out of eight core ASVs were shared suggesting common environmental sources and potential transmission. None of the 46 M. sculpturalis bees analysed harboured known bee pathogens, while microparasite infections were common in A. florentinum, and rare in H. scabiosae. A common shift in the gut microbiota of M. sculpturalis in invaded regions as a response to changed environmental conditions, or a founder effect coupled to population re‐establishment in the invaded regions may explain the observed microbial community profiles and the absence of parasites. While the role of pathogen pressure in shaping biological invasions is still debated, the absence of natural enemies may contribute to the invasion success of M. sculpturalis.

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Section: Resultsmentioning

confidence: 99%

Microbiota and pathogens in an invasive bee: Megachile sculpturalis from native and invaded regions

Tuerlings

Hettiarachchi

Joossens

et al. 2023

Insect Molecular Biology

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“…After individual homogenization, total genomic DNA was extracted by means of a High Pure PCR Template Preparation kit (Roche Diagnostics) and amplified by qPCR using two pair of primers: ApBF1 and ApBR1 - which targets A. bombi as well as Apicystis cryptica DNA, plus ApC500f and ApC666r 5 - which targets only A. cryptica DNA ( Schoonvaere et al, 2020 ) (see Electronic supplementary material for details). DNA fragments obtained with ApBF1 and ApBR1 primers were purified and directly sequenced (ABI 3500 Genetic Analyzer, Applied Biosystems, Foster City, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Detection of Apicystis bombi (Apicomplexa: Neogregarinorida) in carpenter bees of Argentina

Plischuk¹,

Quintana²,

Landa³

et al. 2023

International Journal for Parasitology: Parasites and Wildlife

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“…A single species of apicomplexan protozoa, Apicystis bombi, belonging to the Lipotrophidae family, has been described in A. mellifera . The main host of this parasite appears to be the bumble bee, where it has been described infecting fat-body cells resulting in hypertrophism of the fat body [ 230 , 231 ]. This parasite has so far only been found in honeybees in Japan and Argentina, and studies are scarce [ 232 , 233 ].…”

Section: Description Of Main Bee Pathogens and Molecular Methods For ...mentioning

confidence: 99%

“…The genome of A. bombi has not been sequenced yet, and only 18S rRNA gene sequences are available in GenBank [ 233 , 234 ]. Consequently, PCRs exclusively using this gene as target have been developed and applied to the detection of this pathogen in honey and/or bumble bees [ 231 , 232 , 235 , 236 ].…”

Section: Description Of Main Bee Pathogens and Molecular Methods For ...mentioning

confidence: 99%

Molecular Detection and Differentiation of Arthropod, Fungal, Protozoan, Bacterial and Viral Pathogens of Honeybees

Lannutti

Gonzales

Santos

et al. 2022

Veterinary Sciences

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The honeybee Apis mellifera is highly appreciated worldwide because of its products, but also as it is a pollinator of crops and wild plants. The beehive is vulnerable to infections due to arthropods, fungi, protozoa, bacteria and/or viruses that manage to by-pass the individual and social immune mechanisms of bees. Due to the close proximity of bees in the beehive and their foraging habits, infections easily spread within and between beehives. Moreover, international trade of bees has caused the global spread of infections, several of which result in significant losses for apiculture. Only in a few cases can infections be diagnosed with the naked eye, by direct observation of the pathogen in the case of some arthropods, or by pathogen-associated distinctive traits. Development of molecular methods based on the amplification and analysis of one or more genes or genomic segments has brought significant progress to the study of bee pathogens, allowing for: (i) the precise and sensitive identification of the infectious agent; (ii) the analysis of co-infections; (iii) the description of novel species; (iv) associations between geno- and pheno-types and (v) population structure studies. Sequencing of bee pathogen genomes has allowed for the identification of new molecular targets and the development of specific genotypification strategies.

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Comparison between Apicystis cryptica sp. n. and Apicystis bombi (Arthrogregarida, Apicomplexa): Gregarine parasites that cause fat body hypertrophism in bees

Cited by 8 publications

References 38 publications

Microbiota and pathogens in an invasive bee: Megachile sculpturalis from native and invaded regions

Microbiota and pathogens in an invasive bee: Megachile sculpturalis from native and invaded regions

Detection of Apicystis bombi (Apicomplexa: Neogregarinorida) in carpenter bees of Argentina

Molecular Detection and Differentiation of Arthropod, Fungal, Protozoan, Bacterial and Viral Pathogens of Honeybees

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