Comparison between Alkaline Solubilization Methods and Acid Digestion of Animal Tissue for Determination of Metals by Flame Atomic Absorption Spectrometry

Butik, Mariane; Filho, Irineo Kelte; Peres, Jayme Augusto; Quináia, Sueli Pércio

doi:10.21577/1984-6835.20180073

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…The rat testicle samples were prepared from acid digestion using concentrated HNO 3 as previously described. 45 The determination of AgNP was carried out with electrothermal atomic absorption equipment (model AA 240Z) from Varian (Agilent) with Zeeman background correction and a graphite tube atomizer (GT 120) linked to an autosampler (PSD 120). The analysis was performed using one hollow cathode lamp operating at 328.1 nm with a current of 4 mA and a slit width of 0.5 nm.…”

Section: ■ Experimental Proceduresmentioning

confidence: 99%

Effects of Silver Nanoparticle Exposure to the Testicular Antioxidant System during the Prepubertal Rat Stage

Lopes

Oliveira

Bargi‐Souza

et al. 2019

Chem. Res. Toxicol.

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Humans and environments are constantly exposed to a wide range of commercial products containing silver nanoparticles (AgNPs) in their composition. The hypothalamic-pituitary-testicular (HP-testicular) axis is sensitive to low doses of AgNPs with repercussions in sperm functionality. The oxidative stress may be related to the pathogenesis of sperm alterations because Ag+ ions are released from AgNPs in the corporal fluids. This study aimed to investigate the effects of AgNP exposure in the antioxidant defense system. For this, the transcript expression and the activity of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX), and glutathione reductase (GSR) enzymes were evaluated in the testis of rats exposed during the prepubertal period to increasing doses of AgNPs (1.875, 3.75, 7.5, or 15 μg of AgNPs/kg). The higher dose of AgNPs (15 μg/kg) investigated promoted increases in the activity of CAT, GPX, and GSR enzymes and in the expression of Gpx4 var1 transcript. The exposure to 7.5 μg/kg of AgNP increased the Gpx4 var1 mRNA expression. In the group that received 3.75 μg of AgNP/kg, the expression of Sod1, Gpx4 var2, and Gsr transcripts was decreased while the Gpx4 var1 mRNA expression was augmented. The lower dose of AgNPs tested (1.875 μg/kg) increased the expression of Cat and Gpx4 var1 transcripts. Thus, AgNP alters the expression and activity of the antioxidant enzymes in a nonmonotonic dose–response curve and directly or indirectly modulates the events related to spermatogenesis process.

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Section: ■ Experimental Proceduresmentioning

confidence: 99%

Effects of Silver Nanoparticle Exposure to the Testicular Antioxidant System during the Prepubertal Rat Stage

Lopes

Oliveira

Bargi‐Souza

et al. 2019

Chem. Res. Toxicol.

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“…ISSN: 1696-8352 quercetin (Sigma-Aldrich, 98%) and aluminium chloride (AlCl3) (Êxodo Científica). All the flasks and glassware used were properly decontaminated in nitric acid 5% bath (v/v) for at least 24h and later rinsed with deionized water (Butik, Kelte Filho, Peres, & Quináia, 2018).…”

Section: Instruments and Reagentsmentioning

confidence: 99%

Evaluation of bioaccessibility of metallic ions and antioxidant capacity in Miconia albicans and Senna alexandrina teas

Butik¹,

Andrade²,

Quináia³

2023

OLEL

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Tea is one of the most consumed non-alcoholic beverages, second only to water. Medicinal plants are commonly used because they are beneficial to health. Among the several plants used whit this aim, Miconia albicans (MA), widely used for joint pain, such as rheumatoid arthritis and other inflammatory processes, and Senna alexandrina (SA), indicated for constipation and even for slimming treatments stand out. However, a little number of researches concerned with the chemical composition of these plants can be found in the literature. Thus, this work aimed to determine metallic ions in MA and SA teas using an atomic absorption spectrometer. In vitro experiments with a simulation of the gastrointestinal digestion processes were also performed (to evaluate bioaccessibility) – after in house validation – using gastric and intestinal juice. Total phenolics and total flavonoids of teas and bioaccessible fractions were also quantified. 3 samples of MA and 5 of SA were analyzed. In all samples, the total and bioaccessible concentrations of Ca, Cu and Mg were quantified. The antioxidant activity of the teas showed that, for the total phenolic content, the concentration ranged from 22 ± 1 to 29 ± 3 mg g-1, the bioaccessible fraction was close to 90% for the two studied plants. For total flavonoids, the bioaccessible fractions range from 60% (MA) to 90% (SA).

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“…After defrosted, the biological samples described in Table 1 were cut in fragments of ~2.0 mm, using a glass support and a stainless steel knife to avoid contaminations. The sample preparation procedure using alkaline solubilization with tetramethylammonium hydroxide (TMAH) (Sigma-Aldrich, 25% m v -1 ) was carried out in triplicate out according to methodology described by BUTIK et al 21 . In 0.3 g of tissue canine, 1.0 mL of TMAH and 0.5 mL of water were added; the tissue was then heated in a block digester at 60°C for 1 h.…”

Section: Sample Preparation For F Aas Analysismentioning

confidence: 99%

Comparative Trace Elemental Analysis in Cancerous and Noncancerous Animal Tissues Using Logistic Regression

Butik¹,

Filho²,

Maciel³

et al. 2020

Rev. Virtual Quim.

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Essential metals are important in the normal functioning of the body. However, tissues or organs can be affected when there are inappropriate concentrations of these elements. In the literature, there are works indicating that essential elements may be related to the emergence of neoplasms. Therefore, it is important to determine the metal species in biological samples in order to relate the concentration of these species to the emergence of neoplasms. This study reports the application of the Flame Atomic Absorption Spectroscopy (FAAS) for the quantification of Cu, Fe, Mn and Zn in 31 samples of canine tissue (11 of mammary glands, 4 of pancreas, 3 of skin and 13 of testicles) with and without neoplasms, using alkaline solubilization as sample preparation method. The concentration of these metals was used as co-variables for the construction of models of logistic regression. The concentration of Zn in breast and Fe in pancreas was significant (p < 0.05) in the logistic models. The findings of this study describe that the elements Fe and Zn play a major role in the carcinomas development, since high levels of these metals were observed in neoplastic tissues.

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Comparison between Alkaline Solubilization Methods and Acid Digestion of Animal Tissue for Determination of Metals by Flame Atomic Absorption Spectrometry

Cited by 4 publications

References 17 publications

Effects of Silver Nanoparticle Exposure to the Testicular Antioxidant System during the Prepubertal Rat Stage

Effects of Silver Nanoparticle Exposure to the Testicular Antioxidant System during the Prepubertal Rat Stage

Evaluation of bioaccessibility of metallic ions and antioxidant capacity in Miconia albicans and Senna alexandrina teas

Comparative Trace Elemental Analysis in Cancerous and Noncancerous Animal Tissues Using Logistic Regression

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