Comparison between a cultural procedure using Rappaport-Vassiliadis broth and motility enrichments on modified semisolid Rappaport-Vassiliadis medium for Salmonella detection from food and feed

A comparative study of cultural methods for the detection of Salmonella in feed and feed ingredients

“…According to Van Schothorst and Renaud [ 28 ] a high level of intrinsic flora in the sample, after enrichment, may reduce isolation of Salmonella bacteria on BGA. A comparison between XLD plates and BGA-plates showed that XLD is superior to BGA, due to higher selectivity [ 22 , 29 ], which is in agreement with the results in the present study. Re-incubation of MSRV plates for an additional 24 h did not increase the number of positive samples and an uneven halo was typically observed, not representing growth of Salmonella .…”

“…It has been shown in several studies of naturally infected or artificially contaminated food or faecal samples that MSRV is, in most cases, more sensitive than the standard methods [ 18 - 21 ]. However, there are also studies showing that MSRV is less sensitive compared to other enrichment media [ 19 , 22 ]. In the recent EFSA report about microbial risks in feedingstuffs it is recommended that MSRV or alternative methods should be validated also for use in feed [ 23 ].…”

Rapid detection of Salmonella in foods using a combination of SPRINT TM,MSRV TM and Salmonella Latex TestTM

“…These indexes indicate that the failure in detecting all Samonella positive samples by the proposed method can be charged to the use of MSRV for plating and Salmonella Latex Test for confirmation of Salmonella positive samples. Both systems had already been demonstrated to be very sensitive to detect Salmonella (De Smedt et al, 1986;De Smed, Bolderdijk, 1987;Holbrook et al, 1989;De Zutter, Arnaut-Rollier, 1999;Poppe, Ducan, 1996;O´Donogue, Winn, 1993;De Smedt et al, 1994;Dusch, Altwegg, 1995;Wiberg, Norberg, 1996;Worcman-Barninka et al, 2001), but our results indicate that their combined use cannot be recommended.…”

“…Two of them, the semi-solid Rappaport-Vassiliadis medium (MSRV) and the Oxoid Salmonella Latex Text, are widely used and particularly useful to reduce time and labor in the laboratory (De Smedt et al, 1986;De Smedt, Bolderdijk, 1987;Holbrook et al, 1989. Poppe, Ducan, 1996O´Donogue, Winn, 1993;De Smedt et al, 1994;Dusch, Altwegg, 1995;Wiberg, Norberg, 1996;De Zutter, Arnaut-Rollier, 1999;Worcman-Barninka et al, 2001).…”

Comparison of Culture Methods for Isolation of Salmonella in Yak Fecal Samples

“…The comparison of the rates of positive reactions by each method was statistically analyzed according to the McNemar test [10]. A pair-wise was made using the relationship:…”