Comparison and optimization of conjugated linoleic acid production by Lactobacillus plantarum and Lactobacillus plantarum subsp. plantarum

Abstract

Abstract. Conjugated Linoleic Acid (CLA) was produced from castor oil using washed cells of Lactobacillus plantarum PTCC 1058 and Lactobacillus plantarum subsp. plantarum PTCC1745 as the catalyst. Under the optimal reaction conditions, the washed cells of Lactobacillus plantarum PTCC1058 produced 1661.26 mg of CLA/L reaction mixture (36% yield of production) from 4.6 mg/ml of castor oil after using 15% (w/v) cell for 121 h. The resulting CLA was a mixture of two CLA isomers, cis-9, trans-11 (or trans-9, cis-11)-octadecadienoic acid (CLA1, 44% of total CLA) and trans-10, cis-12-octadecadienoic acid (CLA2, 46% of total CLA). The total production of CLA is extracellular in all of the reactions performed with Lactobacillus plantarum PTCC1058. Also, Lactobacillus plantarum PTCC1745 produced 1590.31 mg of CLA/L reaction mixture (16.5% production yield) from 9.6 mg/ml of castor oil after using 15% (w/v) cell for 121 h. Therefore, Lactobacillus plantarum PTCC1058 was suggested for CLA production due to its more production ability and CLA extracellular production.

Response Surface Methodology for the Optimization of Coco Ethanolamide Production from Coconut Oil

“…Although a value cannot be determined as a recommendation to get the maximum conversion, Fig. 2 shows that overall FAME conversion will increase as the catalyst concentration increases 12 .…”

“…caused by the formation of alcohol as a by-product which will cause the nucleophilic attack and an intermediate giving that can lose the proton to form a new ester. Since the reaction between carbonation and alcohol can produce esters, it has been proposed that this reaction should be performed in the absence of alcohol 12 .…”

“…Coconut fatty acids derived from coconut oil transesterification are then amidized with monoethanolamine to produce coco ethanolamide using homogeneous and heterogeneous catalysts. 12,13 . Monoethanolamine (MEA) or often referred to as 2-aminoethanol or ethanolamine is an amino alcohol which is hygroscopic with an ammonia odor.…”

Extraction and purification of phosphatidylcholine and its potential in nanoliposomal delivery of Eucalyptus citriodora oil

“…Liposomes, consisting of one or more phospholipid (PL) bilayer membranes with an amphipathic behaviour, can deliver both hydrophilic and lipophilic drugs. [ 1,2 ] PL is extracted from lecithin, which is present abundantly and naturally in plant‐derived sources, such as sunflowers and soybeans, and animal‐derived ones, such as egg yolk, marine sources, and milk. [ 3 ] Plant‐derived sources of lecithin are more stable, as they contain fewer polyunsaturated fatty acids.…”

“…Nanoparticles in various forms of polymer‐based nanoparticles, lipid‐based nanoparticles, dendrimers, and inorganic nanoparticles, have been used extensively in modern food and medicine supplementations due to their unique advantages, such as improving drug delivery efficacy and therapeutic index of drug payloads, especially in the case of local antimicrobial treatment. [ 1,13,14 ] Local delivery of antibiotic drugs is an important issue, especially for the treatment of infectious diseases like skin infections, respiratory disorders, and food poisoning. [ 15,16 ] Therefore, effectively delivering antibiotic agents to the desired target is an essential issue in food and medical sciences.…”