Comparison and multivariate statistical analysis of anthocyanin composition in Lycium ruthenicum Murray from different regions to trace geographical origins: The case of China

Wang, Zi-Chao; Yan, Yuzhen; Nisar, Tanzeela; Zou, Li; Yang, Xi; Niu, Pengfei; Sun, Lijun; Guo, Yurong

doi:10.1016/j.foodchem.2017.11.030

Cited by 45 publications

(26 citation statements)

References 38 publications

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“…The anthocyanins were extracted according to the previous method used by Wang et al (2018) with some modifications. About 5.0 g of the samples were extracted triply with 50 mL methanol and formic acid (98:2, v/v).…”

Section: Extraction Of Clm Anthocyaninsmentioning

confidence: 99%

“…HPLC analysis was performed as described in our previous study (Wang et al, 2018) on an UltiMate 3000 HPLC system coupled with an ultraviolet-visible detector, a P680 HPLC pump and an AT-330 thermostatted column compartment (Thermo Scientific, Waltham, USA). CLM anthocyanins (before and after in vitro gastrointestinal digestion) were dissolved in a methanol-formic acid (98:2, v/v) mixture.…”

Section: Identification Of Clm Anthocyaninsmentioning

confidence: 99%

“…The contents of individual CLM anthocyanins were determined according to our previous study (Wang et al, 2018) using the HPLC system by ultraviolet-visible detector. Cyanidin 3-glucoside was used as a reference to quantify the anthocyanins with the concentration ranging from 0 to 600 μg/mL.…”

Section: Quantitative Analysis Of Clm Anthocyaninsmentioning

confidence: 99%

“…(Zheng et al, 2011). Of these components, anthocyanins are considered as the principal component accounted for high antioxidant, biological and anti-tumor properties (Liobikas, Skemiene, Trumbeckaite, & Borutaite, 2016;Wang et al, 2018;Zheng et al, 2011). Due to limited number of natural sources, cultivated Lycium ruthenicum Murray (CLM) is a promising and cost-effective alternative to the wild one.…”

Section: Introductionmentioning

confidence: 99%

“…High-performance liquid chromatography (HPLC) coupled with MS (high-resolution) had been widely used for the identification of anthocyanins in various berry fruits including Lycium ruthenicum Murray. For examples, for the first time, it was used to investigate the anthocyanins composition in wild Lycium ruthenicum Murray (WLM) for elucidating anthocyanins composition of it and for their further utilization as healthy food and natural pigment resource (Zheng et al, 2011); Recently, Wang et al (2018) have adopted it to study the anthocyanins composition of WLM from different provinces and, based on the identification results, established an effective procedure to trace the geographical origins. Additionally, a high-resolution electrospray ionization-time of flight-MS coupled with an HPLC system (HPLC-ESI-ToF-MS/MS) has been proved as an efficient method to identify the composition of WLM anthocyanins including cis-trans isomers (Wang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Effect of in vitro gastrointestinal digestion on the composition and bioactivity of anthocyanins in the fruits of cultivated Lycium ruthenicum Murray

Wang

Nisar

Sun

et al. 2019

CyTA - Journal of Food

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2019) Effect of invitro gastrointestinal digestion on the composition and bioactivity of anthocyanins in the fruits of cultivated Lycium ruthenicum Murray, ABSTRACT The composition of anthocyanins in cultivated Lycium ruthenicum Murray (CLM) before and after in vitro gastrointestinal digestion was determined using an HPLC system coupled with an electrospray ionization-time of flight mass spectrometry. The antioxidant activity and α-glucosidase inhibition of the anthocyanins before and after digestion were also studied. The results showed that most of the anthocyanins in CLM were found in glycosylated form, containing glycoside ligands. After digestion, the glycosylated anthocyanins were transformed to anthocyanidins (without glycolligands) which exhibited higher antioxidant activity comparatively. Interestingly, although the inhibitory activity of anthocyanins against α-glucosidase was increased by in vitro gastrointestinal digestion, as suggested by IC 50 value, the binding affinity of the anthocyanidins to α-glucosidase remained stable, as demonstrated by fluorescence quenching constant. This suggested that the anthocyanidins might have formed the anthocyanidin-α-glucosidase-substrate tertiary complex. Conclusively, in vitro gastrointestinal digestion may enhance the biological activities of CLM anthocyanins, regarding the antioxidant activity and α-glucosidase inhibition.Efecto de la digestión gastrointestinal in vitro sobre la composición y la bioactividad de las antocianinas en los frutos de Lycium ruthenicum Murray cultivados RESUMEN El presente estudio determinó la composición de las antocianinas en Lycium ruthenicum Murray (CLM) cultivada antes y después de la digestión gastrointestinal in vitro. Para ello se utilizó un sistema de HPLC acoplado con una ionización por electrospray-tiempo de propagación-espectrometría de masas. Asimismo, el estudio analizó la actividad antioxidante y la inhibición de la α-glucosidasa de las antocianinas antes y después de la digestión. Los resultados dan cuenta de que la mayoría de las antocianinas de CLM se encontraron en forma glicosilada y contenían ligandos de glucósidos. Después de la digestión, las antocianinas glicosiladas se transformaron en antocianidinas (sin ligandos de glicol) que, en comparación, mostraron mayor actividad antioxidante. Curiosamente, aunque la actividad inhibitoria de la αglucosidasa por las antocianinas aumentó después de la digestión gastrointestinal in vitro, como lo sugiere el valor de IC 50 , la afinidad de la unión de las antocianidinas a la α-glucosidasa se mantuvo estable, como lo demuestra la constante de desactivación de la fluorescencia. Esto permite suponer que las antocianidinas formaron un complejo terciario de sustrato de antocianidina-α-glucosidasa. Se concluye que la digestión gastrointestinal in vitro puede mejorar las propiedades biológicas de las antocianinas CLM, en relación con la actividad antioxidante y la inhibición de la α-glucosidasa. ARTICLE HISTORY

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Section: Extraction Of Clm Anthocyaninsmentioning

confidence: 99%

Section: Identification Of Clm Anthocyaninsmentioning

confidence: 99%

Section: Quantitative Analysis Of Clm Anthocyaninsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of in vitro gastrointestinal digestion on the composition and bioactivity of anthocyanins in the fruits of cultivated Lycium ruthenicum Murray

Wang

Nisar

Sun

et al. 2019

CyTA - Journal of Food

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Ultrasonic extraction of anthocyanins from Lycium ruthenicum Murr. and its antioxidant activity

Liu

Dong

et al. 2020

Food Science & Nutrition

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The ultrasonic extraction (UE) technology, possessed the advantages of effective, energy‐saving, and environmental‐friendly, was applied to extract the anthocyanin from Lycium ruthenicum (LR). The extraction parameters of UE were optimized by response surface methodology (RSM) with Box–Behnken design (BBD). Anthocyanin composition in LR fruits grown in China was systematically identified and quantified by HPLC‐ESI‐MS. The result showed that PRG was the major anthocyanin, and delphinidin, petunidin, and malvidin were the major anthocyanidins in LR fruits. There was the same anthocyanin composition of LR and great variation in anthocyanins content of LR from different areas in China. However, there was no significant difference between wild and cultivated LR in the same region. A clear separation of LR according to geographical origins was revealed by hierarchical cluster analysis (HCA) and principal component analysis (PCA), and the discrimination model for the anthocyanin concentrations were developed using these two analysis methods. Furthermore, on‐line HPLC‐DPPH assay and scavenging activity of three kinds of radicals (DPPH·, ·OH, and ) in vitro were well applied to evaluate the antioxidant activity of the LR anthocyanin extract (LRAE). And its results indicated the LRAE could be a credible antioxidant agent for applications in cosmetics, food, and medicine.

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Transcriptome analysis reveals genes related to the synthesis and metabolism of cell wall polysaccharides in goji berry (Lycium barbarum L.) from various regions

Zhang

Yang

et al. 2023

J Sci Food Agric

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BACKGROUNDIn goji berries (Lycium barbarum L.), the cell wall properties and ripening environment affect fruit quality and their economic benefits. However, the mechanism underlying the cell wall remains to be fully elucidated.RESULTSThe results showed that total sugar content was higher in Qinghai berries (13.87%, P < 0.01), whereas cellulose content peaked in Zhongning berries (28%, P < 0.05). Arabinose, galactose, and galacturonic acid were the principal components of the cell wall polysaccharides in goji berries. Among them, the content of galactose in Zhongning was significantly the highest (P < 0.05). Interestingly, we found that highly expressed β‐glucosidase and lowly expressed endoglucanase led to cellulose accumulation by RNA‐sequencing analysis. The expression analysis results suggested that pectate lyase and pectinesterase enzymes could be major factors related to higher galactose and galacturonic acid contents in Zhongning compared to in Qinghai and Gansu. The starch and sucrose metabolism pathway, pentose and glucuronate interconversions pathway, and galactose metabolism pathway played a significant role in cell wall polysaccharide synthesis and metabolism.CONCLUSIONIn the present study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China. These results might help to clarify the molecular function of the major genes in the cell wall polysaccharides of goji berries and provide a solid foundation for further study. © 2023 Society of Chemical Industry.

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Comparison and multivariate statistical analysis of anthocyanin composition in Lycium ruthenicum Murray from different regions to trace geographical origins: The case of China

Cited by 45 publications

References 38 publications

Effect of in vitro gastrointestinal digestion on the composition and bioactivity of anthocyanins in the fruits of cultivated Lycium ruthenicum Murray

Effect of in vitro gastrointestinal digestion on the composition and bioactivity of anthocyanins in the fruits of cultivated Lycium ruthenicum Murray

Ultrasonic extraction of anthocyanins from Lycium ruthenicum Murr. and its antioxidant activity

Transcriptome analysis reveals genes related to the synthesis and metabolism of cell wall polysaccharides in goji berry (Lycium barbarum L.) from various regions

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