Comparison and Interpretation of Taxonomical Structure of Bacterial Communities in Two Types of Lakes on Yun-Gui plateau of China

Han, Maozhen; Gong, Yanhai; Zhou, Chunyu; Zhang, Junqian; Wang, Zhi; Ning, Kang

doi:10.1038/srep30616

Cited by 26 publications

(22 citation statements)

References 46 publications

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“…These results differ from previous studies, which showed mesotrophic waters to exhibit similarities either to oligotrophic or to eutrophic waters [17,18]. The complete differentiation of the bacterial community in mesotrophic PW from the other samples supports the suggestion that extreme water physicochemical conditions, like the low temperatures recorded in this study, can shape communities and select taxa that cope well with harsh environment [59]. Actually, NMDS analysis indicated temperature as a factor affecting the community structure of PW.…”

Section: Discussioncontrasting

confidence: 99%

Diversity of Bacteria in Lakes with Different Chlorophyll Content and Investigation of Their Respiratory Activity through a Long-Term Microcosm Experiment

Karayanni

Macingo

Tolis

et al. 2019

Water

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Bacterial community structure and metabolism are critical factors for ecosystem functioning since they affect remineralization of nutrients and carbon flow. We used Illumina sequencing of 16SrRNA V3-V4 regions to investigate whether bacterial assemblage composition differs between four samples from two lakes in the geographic region of Epirus (Greece) characterized by distinct oligotrophic to eutrophic/hypereutrophic conditions as revealed by chlorophyll-a values. We found high similarity (>60%) for bacterial assemblages recovered from the two lakes when eutrophic/hypereutrophic conditions prevailed. Distinct bacterial communities appeared in oligotrophic and mesotrophic waters. Low temperature was occasionally an important factor in shaping the bacterial community. In parallel, microcosm experiments were performed to estimate respiration rates of bacterioplankton at in situ temperature and under a 2 °C temperature increase scenario. Differently assembled communities were found to display similar rates except under hypereutrophic conditions when respiration increased significantly, leading to hypoxic conditions. Temperature increase did not affect respiration rates. Overall this study indicated a clear differentiation of bacterial communities between sites of different trophic state. However, different communities responded similarly under a specific range of chlorophyll-a values and resisted small scale temperature perturbations. Different results were found for hypereutrophic conditions and this has implications for ecosystems functioning, given the increasing occurrence of eutrophication events.

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Section: Discussioncontrasting

confidence: 99%

Diversity of Bacteria in Lakes with Different Chlorophyll Content and Investigation of Their Respiratory Activity through a Long-Term Microcosm Experiment

Karayanni

Macingo

Tolis

et al. 2019

Water

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“…We speculate that the decay of these plants during winter subtantially increases organic matter, total nitrogen (Sand-jensen 1998), and total phosphorus(Horppila and Nurminen 2003) in sediment samples. Hence, as expected from previous research, we found that both water and sediment microbial community structure was correlated with TP and TN concentration(Han et al 2016, Takamura N 2003). Moreover, in water samples, we observed that Bacillus flexus was strongly correlated with TN, NH 4 + -N, PO 4 3- -P, and oxytetracycline.…”

Section: Discussionsupporting

confidence: 90%

“…Approximately 5-50 ng of DNA was used as template for amplifying the V4-V5 hypervariable region of the 16S rRNA gene of microbiota for each sample. Sequences for the forward and reverse primers are "GTGYCAGCMGCCGCGGTAA" and "CTTGTGCGGKCCCCCGYCAATTC", respectively(Han et al 2016). The sequencing library was constructed using a MetaVx™ Library Preparation kit (GENEWIZ, Inc., South Plainfield, and NJ, USA).…”

Section: Methodsmentioning

confidence: 99%

Agricultural Pollution Risks Influence Microbial Ecology in Honghu Lake

Han

Dsouza

Zhou

et al. 2018

Preprint

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BackgroundAgricultural activities, such as stock-farming, planting industry, and fish aquaculture, can influence the physicochemistry and biology of freshwater lakes. However, the extent to which these agricultural activities, especially those that result in eutrophication and antibiotic pollution, effect water and sediment-associated microbial ecology, remains unclear.MethodsWe performed a geospatial analysis of water and sediment associated microbial community structure, as well as physicochemical parameters and antibiotic pollution, across 18 sites in Honghu lake, which range from impacted to less-impacted by agricultural pollution. Furthermore, the co-occurrence network of water and sediment were built and compared accorded to the agricultural activities.ResultsPhysicochemical properties including TN, TP, NO3--N, and NO2--N were correlated with microbial compositional differences in water samples. Likewise, in sediment samples, Sed-OM and Sed-TN correlated with microbial diversity. Oxytetracycline and tetracycline concentration described the majority of the variance in taxonomic and predicted functional diversity between impacted and less-impacted sites in water and sediment samples, respectively. Finally, the structure of microbial co-associations was influenced by the eutrophication and antibiotic pollution.ConclusionThese analyses of the composition and structure of water and sediment microbial communities in anthropologically-impacted lakes are imperative for effective environmental pollution monitoring. Likewise, the exploration of the associations between environmental variables (e.g. physicochemical properties, and antibiotics) and community structure is important in the assessment of lake water quality and its ability to sustain agriculture. These results show agricultural practices can negatively influence not only the physicochemical properties, but also the biodiversity of microbial communities associated with the Honghu lake ecosystem. And these results provide compelling evidence that the microbial community can be used as a sentinel of eutrophication and antibiotics pollution risk associated with agricultural activity; and that proper monitoring of this environment is vital to maintain a sustainable environment in Honghu lake.

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“…Previous studies mainly focused on the bacterial diversity in lake sediments using traditional isolation methods [27,28] and conventional DNA-based molecular methods (e.g., DGGE, T-RFLP, Q-PCR, FISH, RAPD, clone libraries) [29][30][31][32][33][34]. Recently, 16S rRNA sequencing on the Illumina MiSeq platform has been able to provide more detailed information about microbial community diversity and structure [35][36][37][38]. This technique has been widely used in the early detection of aquatic invasion and in investigations of water biodiversity [38].…”

Section: Introductionmentioning

confidence: 99%

Phytoplankton and Bacterial Community Structure in Two Chinese Lakes of Different Trophic Status

et al. 2019

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Phytoplankton are the primary producers at the basis of aquatic food webs, and bacteria play an important role in energy flow and biochemical cycling in aquatic ecosystems. In this study, both the bacterial and phytoplankton communities were examined in the oligotrophic Lake Basomtso and the eutrophic Lake South (China). The results of this study showed that the phytoplankton density and diversity in the eutrophic lake were higher than those in the oligotrophic lake. Furthermore, Chlorophyta (68%) and Cryptophyta (24%) were the dominant groups in the eutrophic lake, while Bacillariophyta (95%) dominated in the oligotrophic lake. The bacterial communities in the waters and sediments of the two lakes were mainly composed of Proteobacteria (mean of 32%), Actinobacteria (mean of 25%), Bacteroidetes (mean of 12%), and Chloroflexi (mean of 6%). Comparative analysis showed that the abundance of bacteria in the eutrophic lake was higher than that in the oligotrophic lake (p < 0.05), but the bacterial diversity in the oligotrophic lake was higher than that in the eutrophic lake (p < 0.05). Finally, the bacterial abundance and diversity in the sediments of the two lakes were higher than those in the water samples (p < 0.05), and the Latescibacteria and Nitrospinae groups were identified only in the sediments. These results suggest that both the phytoplankton and bacterial communities differed considerably between the oligotrophic lake and the eutrophic lake.

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Comparison and Interpretation of Taxonomical Structure of Bacterial Communities in Two Types of Lakes on Yun-Gui plateau of China

Cited by 26 publications

References 46 publications

Diversity of Bacteria in Lakes with Different Chlorophyll Content and Investigation of Their Respiratory Activity through a Long-Term Microcosm Experiment

Diversity of Bacteria in Lakes with Different Chlorophyll Content and Investigation of Their Respiratory Activity through a Long-Term Microcosm Experiment

Agricultural Pollution Risks Influence Microbial Ecology in Honghu Lake

Phytoplankton and Bacterial Community Structure in Two Chinese Lakes of Different Trophic Status

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