Comparison and improvement of methods for determining soil dehydrogenase activity by using triphenyltetrazolium chloride and iodonitrotetrazolium chloride

Friedel, Jürgen K.; Mölter, Kerstin; Fischer, Walter

doi:10.1007/bf00570631

Cited by 82 publications

(42 citation statements)

References 8 publications

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“…Soil dehydrogenase activity (μg TPF/g dry soil/24 hours) was analyzed by the reduction of triphenyl tetrazolium chloride (TTC) to triphenyl formazan (TPF) and as described by Friedel et al (1994). Briefly, five grams of fresh soil were incubated for 24 hours at 37°C in 5 ml of a TTC solution (5 g TTC in 0.2 mol/L Tris-HCl buffer, pH 7.4).…”

Section: Field Experimentsmentioning

confidence: 99%

Role of Endophytic Pseudomonas as Plant Growth Promoters Under Desert Condition

Omer

2016

Egyptian Journal of Desert Research

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lant growth promoting endophytes enhance the plant growth using multiple metabolic activities directed and contribute to the growth, health and development of plant. A total of 46 strains isolated from the rhizosphere of maize, sorghum, barley and wheat plants were screened for their efficiency as plant growth promoting endophytes. Bacterial isolates induced the highest length and weight of maize seedlings on Hoagland media were selected for further investigations. All selected endophytic bacteria possess HCN and indole acetic acid production, while only four isolates showed phosphate dissolving activities. Using Chrome Azurol S plates revealed that all selected isolates were positive to siderophore production. For characterization of siderophores, ferrate hydroxamate type were detected in five bacterial isolates, ferrate catecholate type detected in only three isolates, while no carboxylate siderophores were recorded for any isolates. HPLC analysis of the highest active two isolates revealed the presence of indole acetic acid and indole butyric acid. Phylogenetic analysis of 16S rRNA sequence of the two isolates showed maximum sequence similarity with Pseudomonas aeruginosa strain PAO1 and Pseudomonas geniculata strain ATCC 19374.A field experiment was conducted at El-Qantara Sharq experimental station, Desert Research Center (DRC) for evaluation of Ps. aeruginosa and Ps. geniculata as plant growth promoting endophytes on maize. Bacterial inoculation recorded an increase in biological yield reaching 55.2 and 33% over control for Ps. geniculata and Ps. aeruginosa, respectively. Concerning to chemical constituent of maize grains, inoculation process significantly increased the carbohydrate and oil % with about 4.6 and 4.2% for both bacterial strains. While no change in endophytic Pseudomonads count were detected inside the root of treated plants comparing to control , the inoculation P

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Section: Field Experimentsmentioning

confidence: 99%

Role of Endophytic Pseudomonas as Plant Growth Promoters Under Desert Condition

Omer

2016

Egyptian Journal of Desert Research

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“…Dehydrogenase (DHA) was determined as described in Friedel et al (1994) after soil incubation at 37°C for 24 h with 2,3,5-triphenyl-tetrazolium chloride (TTC) and the red color of triphenylformazan (TPF) absorbance was measured at 546 nm using spectrophotometer. Catalase (CAT) activity was measured by titration of the excess of as a substrate at 37°C in Tris buffer (pH = 9) and the absorbance of blue color was checked at 578 nm by the spectrophotometer (Tabatabai and Bremner, 1972).…”

Section: Basic Soil Physical and Chemical Analysesmentioning

confidence: 99%

Microbiological Indicators of a Clayey Soil Planted with Wheat (Triticum aestivum L.) as Affected by Potassium Fertilization and Different Water Regimes

Ali¹,

Meihy²

2015

Research J. of Soil Biology

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“…The former parameter was estimated by the population size of total aerobic heterotrophic bacteria (THB), according to the most probable number (MPN) method described by Yu et al (2005). The activities of dehydrogenase (DHA) and catalase (CAT), the two common oxidoreductase enzymes in microorganisms indicating the oxidative microbial activity and antioxidant potential under pollutant stress, respectively (Friedel et al, 1994;Sies, 1997) were also measured. Dehydrogenase activity (DHA) was determined based on the reduction of iodonitrotetrazolium chloride (INT) to iodonitrotetrazolium formazan (INTF), following the standard method by Johnsen et al (2002a) with minor modifications.…”

Section: Sample Analysismentioning

confidence: 99%