1997
DOI: 10.1111/j.1600-0773.1997.tb00028.x
|View full text |Cite
|
Sign up to set email alerts
|

Comparison and in vivo Relevance of Two Different in vitro Head Space Metabolic Systems: Liver S9 and Liver Slices

Abstract: Ahstruct:In vitro systems of high biological organization. e.g. containing intact hepatocytes. have been considered as more reliable for metabolic studies and in vivo predictions of toxicokinetics than subcellular systems. For this reason, the kinetics and metabolism of low-molecular-weight volatile chemicals, i.e. head space elimination of substrate and formation of metabolites, were compared in liver S9 and in liver slices. Two substrates. toluene and n-hexane, were used as they represent differences in meta… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1

Citation Types

0
8
0

Year Published

1998
1998
2012
2012

Publication Types

Select...
5
1

Relationship

1
5

Authors

Journals

citations
Cited by 13 publications
(8 citation statements)
references
References 33 publications
0
8
0
Order By: Relevance
“…In particular, in vitro data for humans can then be extrapolated to in vivo human predictions by assuming that the same relationship that successfully describes the in vitro to in vivo relationship in animals effectively converts the human in vitro data to the in vivo situation. There are several examples of successful application based on appropriate in vitro-in vivo scaling methods (Reitz et al, 1989(Reitz et al, , 1996bKedderis and Held, 1996;Mortensen et al, 1997;Mortensen and Nilsen, 1998;Lipscomb et al, 1998Lipscomb et al, , 2003Cole et al, 2001;Hissink et al, 2002;U.S. EPA, 2006b), although the extrapolation of in vitro data to the intact animal is not always straightforward (Haddad et al, 1997(Haddad et al, , 1998.…”
Section: Biochemical Parametersmentioning
confidence: 98%
“…In particular, in vitro data for humans can then be extrapolated to in vivo human predictions by assuming that the same relationship that successfully describes the in vitro to in vivo relationship in animals effectively converts the human in vitro data to the in vivo situation. There are several examples of successful application based on appropriate in vitro-in vivo scaling methods (Reitz et al, 1989(Reitz et al, , 1996bKedderis and Held, 1996;Mortensen et al, 1997;Mortensen and Nilsen, 1998;Lipscomb et al, 1998Lipscomb et al, , 2003Cole et al, 2001;Hissink et al, 2002;U.S. EPA, 2006b), although the extrapolation of in vitro data to the intact animal is not always straightforward (Haddad et al, 1997(Haddad et al, , 1998.…”
Section: Biochemical Parametersmentioning
confidence: 98%
“…Calculations were based on the integrated peak areas. All head space concentrations were calculated on the basis of a minimum of three freshly prepared calibration standards covering the linear range of each analytical series (Mortensen et al 1997). Linearity (r2>0.996) was obtained for all solvents up to 5000 ppm.…”
Section: Pieparution and Incubationsmentioning
confidence: 99%
“…Metabolic rates (nmol/g liver wet weight/ min.) were calculated on the basis of disappearance of the parent chemical (substrate) from head space or liver S9 after correction for the disappearance rate from a concomitant incubation with heat inactivated liver S9 according to Mortensen et al (1997). The metabolic rate represented the amount substrate metabolized after incubation for 30 min.…”
Section: Pieparution and Incubationsmentioning
confidence: 99%
See 2 more Smart Citations