Comparison and efficiency of two membranes (modified MF and UF) during enzymatic hydrolysis of haemoglobin in a membrane bioreactor

Use of response surface methodology to optimise the hydrolysis of whey protein isolate in a tangential flow filter membrane reactor

“…We have since developed a model to relate the partitioning into permeate and retentate in which we quantify the total liberated a-NH 2 groups based on its reaction chemistry with the TNBS. Although other EMR applicants have used the pH-stat method (Bressollier et al, 1988;Mokrane, Belhocine, & Mameri, 2006) it is doubtful whether the results reflect the actual EMR hydrolysis scenario because we have since demonstrated that the pH-stat exaggerated the DH when compared to the TNBS in our modification (unpublished results). The amount of PA soluble in 10% TCA fitted poorly into the model.…”

Comparison of a modified spectrophotometric and the pH-stat methods for determination of the degree of hydrolysis of whey proteins hydrolysed in a tangential-flow filter membrane reactor

“…In, Hee, and Oh (2002) used an osmometric method to determine the DH of only the permeate, while other workers who studied EMR hydrolysis of proteins did not report any determination of the DH at all (Chiang, Shih, & Chu, 1999;D'Alvise, Lesueur-Lambert, Fertin, Dhulster & Guillochon, 2000;Deeslie & Cheryan, 1981;Je, Kim, & Kim, 2005;Mannheim & Cheryan, 1990;Martin-Orue, Henry, & Bouhallab, 1999;Perea & Ugalde, 1996). Meanwhile Bressollier, Petit, and Julien (1988), Lin, Chiang, Cordle, and Thomas (1997) and Mokrane, Belhocine, and Mameri (2006) controversially used the pH-stat method to calculate the DH of EMR hydrolysis processes, which likely led to an exaggeration of the final DH in the EMR, owing to leakage of the alkali used to regulate the pH during hydrolysis in membrane reactors.…”

Enzyme membrane reactor in isolation of antioxidative peptides from oil industry waste: A comparison with non-peptidic antioxidants

“…Its operation simulates size exclusion chromatography (Ghosh, 2002;Prazeres, 1997;Reis & Zydney, 2001;Zeng & Ruckenstein, 1999), thus providing a high throughput, not limited by diffusional restrictions. Various proteins have already been hydrolyzed effectively using a variety of enzymes in EMRs, to get desired bioactive peptides (Belhocine et al, 2000;Cheison, Wang, & Xu, 2006Guadix et al 2006;Mokrane, Belhocine, & Mameri, 2006).…”